GIMLET Home Page

 

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Lastest version

Update*

Download complete package

GIMLET v1.3.3

Download (.exe) 1.16 Mo

Download (.zip) 2,3 Mo

*just replace the new .exe file in the Gimlet folder on you disk!

 

Download Gimlet Software Guide version 1.3.2 (pdf, 265 Ko)

Download paper about Gimlet published (at present only on-line) in Molecular Ecology Notes (pdf, 114 Ko)

 

Update events

Versions

What's new?



v1.3.2-> v1.3.3

February 2004


Bugs have been corrected in the Identification/Regroup genotypes function (genotypes were not correctly assigned to the samples. TRY THE NEW VERSION IF YOU HAVE USED POOLING FUNCTION ON PREVIOUS DATA SETS.





v1.3.1 -> v1.3.2

October 2003


New features for the estimation of population size with the rarefaction curve method (see pp 15-18 of the Guide):

  • the equation used in Eggert et al. (2003) was added,

  • user can choose to print the results for the parameter b of the equations,

  • user can choose several distribution parameters (mean, standard deviation, median, minimim, maximum) to describe the distribution of the estimates of a and b parameters of the equations over all iterations,

  • user can choose the number of iterations when calling the script file in R,

  • R will try at least 5 times to estimate the asymptote for each iteration of difficult to fit* data sets before skipping the iteration and begin the following iteration calculation (*Hard to fit data sets are for example data where the samples are sampled only once. The data sets where samples are sampled several times each are analysed once and the result is printed,

  • user can print the observed and predicted rarefaction curves or the histogram of the number of capture per sample.



Program can print more output files in the "Estimate Error Rates" module: file to test the bias in ADO in favor of short or long alleles, file with all detailed errors

 

v1.3.0 -> v1.3.1

February 2003

 

Bugs in Kinship module have been corrected.

New features in Kinship module: the determination of the kinship could be limited to some individuals (pp 7-8 in the Guide).

v1.2.0 -> v1.3.0

January 2003

Estimation error rate: the error types have been changed. Allele dropout and false allele are still available, however the double and complete error types have been differenciated in 5 distinctive types.

A new method for the construction of consensus genotypes is available. This method is based on the calculation of relative probabilities for potential genotypes using the error rates estimated above.

Bug correction: previous version did not allow the use of two-digit allele higher than 50. This bug has been corrected.

v1.0.1 -> v1.0.2

August 2002

It is now possible to treat missing allele as any other alleles or as distinctive alleles (This is done for Regrouping task; it will be done for Identification soon).

v1.0.0 -> v1.0.1

May 2002

Several bugs were fixed in Calculator module (allele frequencies estimation, Consensus construction) and Identification module (kinship)

New options in Kinship where standard deviations can be set for Life History Traits. Either one parent or pair of parents can be determined whereas always pair of parents before update.

 

What is GIMLET?

GIMLET (Genotypic Identification with MultiLocus Tags) is a software dedicated for geneticists who work on individual identification using molecular tags in diploid species. In this context, molecular tags are composite genotypes at multiple loci, each composed by two alleles. Thus, N pairs of coding values compose the molecular tag for a sample. Coding values could be for example two-digits-numbers designing alleles (e.g. 01, 02, 03 for first, second and third alleles) or could be allele size (three-digits-number) for VNTRs loci (e.g. 242, 244, 246 for first, second and third alleles). GIMLET performs following tasks: (i) estimating error rates during genotyping and constructing consensus genotypes from repeated genotyping, (ii) regrouping identical genotypes, (iii) identifying one or several genotype(s) comparing it (them) with references, (iv) determining parentage between individuals (kinship) (v) estimating several parameters producing data to estimate several parameters (allelic frequencies, Probability of Identity, population size).

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Configuration

GIMLET is a Windows based software compiled with Visual Basic. You can install GIMLET on any Windows® compatible operating system. The installation of GIMLET required about 4 Mo in hard disk (about 1Mo in the folder Gimlet and 3 Mo for system files).

All input and output files are text files (ASCII, space and tabulation separator), which could be written and read with any text editor (such as Word, WordPad, NoteBook) or Excel. These file are under GENEPOP format. Gimlet accepts all file extension.

Questions, comments and bugs reports should be directed to valiere@biomserv.univ-lyon1.fr, indicating in the subject of your email "Gimlet comments". All messages and responses will be redirect to any registered persons.

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Installation

Package Gemini v132.zip is composed by Setup.exe file and all dependancy files needed for the installation of GIMLET, Help file. For installing GIMLET on your computer, double-click on Setup.exe (open Setup.zip using WINZIP available at http://www.winzip.com/) and follow the instructions. Installation requires about 4 Mo disk space (about 1Mo in the folder GIMLET and 3 Mo for system files). After installation, go on your Staring Menu/ Programs to launch GIMLET (Gimlet icon), or go in the Gimlet folder and double click on the Gimlet.exe to run GIMLET.

 

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Software and home page by Nathaniel Valière

at the Laboratoire de Biométrie et de Biologie Evolutive

Updated February 6, 2003