Six provenances of Acacia auriculformis have been tested for their ectomycorrhizal dependency with 2 indigenous strains of Pisolithus sp. Moreover the endomycorrhizal and rhizobial symbiosis, the multiplication of plant parasitic nematodes have been quantified. The results showed significant variations in the growth rate between provenances. The both compatibility and dependency with the ectomycorrhizal strains were also different among the provenances. Three main genera of nematodes have been identified with, in particular, S. cavenessi which is one of the most pathogenic nematode of soudano-sahelian crops. Endomycorrhizal structures were sometimes detected on the root systems but no nodules have been observed for all the provenances. The use of these A. auriculiformis provenances in an agroforestry system was discussed.