P. penetrans populations isolated from diffrent geographical areas were screened for their polymorphism using a immunofluorescence method and their ability to attach different lines of Meloidogyne spp.. The results show a high degree of variability between these populations which is different from the immunological study and from the attachment tests. However the immunoprofiles have divided the populations in agreement with their geographical sources. The efficiency of these P. penetrans populations have been tested in a glasshouse experiment with M. incognita originating from Senegal and A. holosericea. The results show that the population PP16 isolated in a senegalese soil has involved the better development of the seedlings and has had the high rate of sporulation. The difficulties to use the actinomycete in biological control are discussed regarding the high degree of variability of this parasito•d and the impact of soil factors.