Striga hermonthica (Del.) Benth is an obligate root hemi-parasite of several cereals. This effect on the culture is the main constraint of food production in sub-Saharan Africa. Various control methods have been already proposed, but the infestation of these parasitic plants persists. An appropriated method to Striga management adapted for the African farmer is so need. In this study, we suggested the amendment of soil infested by this phytoparasite with Cubitermes mound powder, as chemical amendment and natural microbial inoculums, to promote plant growth and to reduce damage by Striga hermonthica on sorghum (Sorghum bicolor L.) culture. The influence of Cubitermes mound powder on the development of several microbial groups (arbuscular mycorrhizal fungi, actinomycetes, saprophytic fungi) in a pot experiment with sorghum cultured in a sandy soil highly infested by S. hermonthica was investigated. Sorghum growth was clearly enhanced in amended samples and the mycorrhizal colonization of sorghum plants were significantly higher in the soil amended with Cubitermes powder than those recorded in the control treatment. Mycorrhizal colonization was negatively correlated with the number of emerged Striga per pot and positively correlated with Sorghum growth. We noted that the number of emerged Striga in amended pots was significantly decreased and Cubitermes mound suspensions did not affected the Striga seed germination in-vitro conditions. It suggests that the amendment of Cubitermes powder reduce the infestation of S. hermonthica by the means of soil microbial. The Cubitermes mound powder effect against Striga development and its influence on the diversity and development of soil microbial communities was discussed.