(data stored in SCRATCH zone)

SWISSPROT: A0A0F6BHQ8_GEOS0

ID   A0A0F6BHQ8_GEOS0        Unreviewed;      1199 AA.
AC   A0A0F6BHQ8;
DT   24-JUN-2015, integrated into UniProtKB/TrEMBL.
DT   24-JUN-2015, sequence version 1.
DT   08-MAY-2019, entry version 25.
DE   RecName: Full=DNA-directed RNA polymerase subunit beta' {ECO:0000256|HAMAP-Rule:MF_01322};
DE            Short=RNAP subunit beta' {ECO:0000256|HAMAP-Rule:MF_01322};
DE            EC=2.7.7.6 {ECO:0000256|HAMAP-Rule:MF_01322};
DE   AltName: Full=RNA polymerase subunit beta' {ECO:0000256|HAMAP-Rule:MF_01322};
DE   AltName: Full=Transcriptase subunit beta' {ECO:0000256|HAMAP-Rule:MF_01322};
GN   Name=rpoC {ECO:0000256|HAMAP-Rule:MF_01322};
GN   OrderedLocusNames=GY4MC1_0103 {ECO:0000313|EMBL:ADP72959.1};
OS   Geobacillus sp. (strain Y4.1MC1).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Geobacillus.
OX   NCBI_TaxID=581103 {ECO:0000313|EMBL:ADP72959.1, ECO:0000313|Proteomes:UP000002034};
RN   [1] {ECO:0000313|EMBL:ADP72959.1, ECO:0000313|Proteomes:UP000002034}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Y4.1MC1 {ECO:0000313|EMBL:ADP72959.1,
RC   ECO:0000313|Proteomes:UP000002034};
RG   US DOE Joint Genome Institute;
RA   Lucas S., Copeland A., Lapidus A., Cheng J.-F., Bruce D., Goodwin L.,
RA   Pitluck S., Chertkov O., Zhang X., Detter J.C., Han C., Tapia R.,
RA   Land M., Hauser L., Jeffries C., Kyrpides N., Ivanova N.,
RA   Ovchinnikova G., Brumm P., Mead D., Woyke T.;
RT   "Complete sequence of chromosome of Geobacillus sp. Y4.1MC1.";
RL   Submitted (OCT-2010) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription
CC       of DNA into RNA using the four ribonucleoside triphosphates as
CC       substrates. {ECO:0000256|HAMAP-Rule:MF_01322,
CC       ECO:0000256|RuleBase:RU004279, ECO:0000256|SAAS:SAAS00054030}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:11128, Rhea:RHEA-
CC         COMP:11129, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557,
CC         ChEBI:CHEBI:83400; EC=2.7.7.6; Evidence={ECO:0000256|HAMAP-
CC         Rule:MF_01322, ECO:0000256|RuleBase:RU004279,
CC         ECO:0000256|SAAS:SAAS01121830};
CC   -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1
CC       beta' and 1 omega subunit. When a sigma factor is associated with
CC       the core the holoenzyme is formed, which can initiate
CC       transcription. {ECO:0000256|HAMAP-Rule:MF_01322}.
CC   -!- SIMILARITY: Belongs to the RNA polymerase beta' chain family.
CC       {ECO:0000256|HAMAP-Rule:MF_01322, ECO:0000256|RuleBase:RU004279}.
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DR   EMBL; CP002293; ADP72959.1; -; Genomic_DNA.
DR   RefSeq; WP_013399827.1; NC_014650.1.
DR   EnsemblBacteria; ADP72959; ADP72959; GY4MC1_0103.
DR   GeneID; 29237261; -.
DR   KEGG; gmc:GY4MC1_0103; -.
DR   KO; K03046; -.
DR   OMA; QGVEIND; -.
DR   OrthoDB; 4421at2; -.
DR   BioCyc; GSP581103:G1GOQ-130-MONOMER; -.
DR   Proteomes; UP000002034; Chromosome.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR   Gene3D; 1.10.132.30; -; 1.
DR   Gene3D; 1.10.274.100; -; 1.
DR   HAMAP; MF_01322; RNApol_bact_RpoC; 1.
DR   InterPro; IPR012754; DNA-dir_RpoC_beta_prime.
DR   InterPro; IPR000722; RNA_pol_asu.
DR   InterPro; IPR006592; RNA_pol_N.
DR   InterPro; IPR007080; RNA_pol_Rpb1_1.
DR   InterPro; IPR007066; RNA_pol_Rpb1_3.
DR   InterPro; IPR042102; RNA_pol_Rpb1_3_sf.
DR   InterPro; IPR007083; RNA_pol_Rpb1_4.
DR   InterPro; IPR007081; RNA_pol_Rpb1_5.
DR   InterPro; IPR038120; Rpb1_funnel_sf.
DR   Pfam; PF04997; RNA_pol_Rpb1_1; 1.
DR   Pfam; PF00623; RNA_pol_Rpb1_2; 2.
DR   Pfam; PF04983; RNA_pol_Rpb1_3; 1.
DR   Pfam; PF05000; RNA_pol_Rpb1_4; 1.
DR   Pfam; PF04998; RNA_pol_Rpb1_5; 1.
DR   SMART; SM00663; RPOLA_N; 1.
DR   TIGRFAMs; TIGR02386; rpoC_TIGR; 1.
PE   3: Inferred from homology;
DR   PRODOM; A0A0F6BHQ8.
DR   SWISS-2DPAGE; A0A0F6BHQ8.
KW   Coiled coil {ECO:0000256|SAM:Coils};
KW   Complete proteome {ECO:0000313|Proteomes:UP000002034};
KW   DNA-directed RNA polymerase {ECO:0000256|HAMAP-Rule:MF_01322,
KW   ECO:0000256|RuleBase:RU004279, ECO:0000256|SAAS:SAAS00442970,
KW   ECO:0000313|EMBL:ADP72959.1};
KW   Nucleotidyltransferase {ECO:0000256|HAMAP-Rule:MF_01322,
KW   ECO:0000256|RuleBase:RU004279, ECO:0000256|SAAS:SAAS00442967};
KW   Transcription {ECO:0000256|HAMAP-Rule:MF_01322,
KW   ECO:0000256|RuleBase:RU004279, ECO:0000256|SAAS:SAAS00442998};
KW   Transferase {ECO:0000256|HAMAP-Rule:MF_01322,
KW   ECO:0000256|RuleBase:RU004279, ECO:0000256|SAAS:SAAS00443006}.
FT   DOMAIN      224    503       RPOLA_N. {ECO:0000259|SMART:SM00663}.
FT   COILED      181    201       {ECO:0000256|SAM:Coils}.
SQ   SEQUENCE   1199 AA;  134918 MW;  DD4E31587A0E2403 CRC64;
     MLDVNKFEYM KIGLASPEKI RSWSYGEVKK PETINYRTLK PEKDGLFCER IFGPTKDWEC
     HCGKYKRVRY KGVVCDRCGV EVTRSKVRRE RMGHIELAAP VSHIWYFKGI PSRMGLVLDM
     SPRALEEVIY FASYVVTDPG DTPLEKKQLL SEKEYRAYRE KYGQSFQASM GAEAIKKLLQ
     DIDLEKEVAT LKEELKTAQG QRRARIIKRL EVLEAFRNSG NDPAWMVLDV LPVIPPELRP
     MVQLDGGRFA TSDLNDLYRR VINRNNRLKR LLDLGAPSII VQNEKRMLQE AVDALIDNGR
     RGRPVTGPGN RPLKSLSHML KGKQGRFRQN LLGKRVDYSG RSVIVVGPNL KMYQCGLPKE
     MALELFKPFV MKELVERGLA HNIKSAKRKI ERVHPEVWDV LEDVIKEHPV LLNRAPTLHR
     LGIQAFEPTL VEGRAIRLHP LVCTAYNADF DGDQMAVHVP LSAEAQAEAR LLMLAAQNIL
     NPKDGKPVVT PSQDMVLGNY YLTMEREGAI GEGMVFKDTD EALLAYHNGY VHLHSRIAVH
     AGSLKNETFT EEQNNKLLIT TVGKLIFNEI LPNSFPYINE PTTENIEGRT PDKYFLDKGV
     NVKEEIRKRE LVPPFKKKVL GQIIAEVFKR FKITETSKML DRMKDLGFQY STKAGITIGV
     ADIVVLPEKQ EILEEAQTKV DTVLKQFRRG LITDEERYER VISIWSAAKD KIQDRLMKSL
     DKRNPIFMMS DSGARGNASN FTQLAGMRGL MANPAGRIIE LPIKSSFREG LTVLEYFIST
     HGARKGLADT ALKTADSGYL TRRLVDVAQD VIVREEDCGT DRGILARALT DGTEVIVKLE
     ERLVGRYAQK TVRHPETGEI LVRKDEMITE DIANAIVKAG IKEVWIRSAF TCNTRHGVCK
     KCYGRNLATG AEVEVGEAVG IIAAQSIGEP GTQLTMRTFH TGGVAGDDIT QGLPRVQELF
     EARNPKGQAV ISEIDGIVTS ISETRDRQYE IVVKGEVETR SYIAPYNARL KVEEGQQVER
     GQELTEGSID PKQLLKVRDV TSVQEYLLRE VQKVYRMQGV EISDKHIEVM VRQMLRKVRI
     IDAGDTDVLP GTLLDVHQFT DVNEKALLEG KRPATARPVL LGITKASLET DSFLSAASFQ
     ETTRVLTDAA IKGKRDELLG LKENVIIGKL VPAGTGMARY RKVKPVVKKE ENEDAVTSK
//

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