(data stored in SCRATCH zone)

SWISSPROT: A0A0H2ZK52_PSEAB

ID   A0A0H2ZK52_PSEAB        Unreviewed;       317 AA.
AC   A0A0H2ZK52;
DT   16-SEP-2015, integrated into UniProtKB/TrEMBL.
DT   16-SEP-2015, sequence version 1.
DT   07-JUN-2017, entry version 16.
DE   RecName: Full=Glutathione synthetase {ECO:0000256|HAMAP-Rule:MF_00162};
DE            EC=6.3.2.3 {ECO:0000256|HAMAP-Rule:MF_00162};
DE   AltName: Full=GSH synthetase {ECO:0000256|HAMAP-Rule:MF_00162};
DE            Short=GSH-S {ECO:0000256|HAMAP-Rule:MF_00162};
DE            Short=GSHase {ECO:0000256|HAMAP-Rule:MF_00162};
DE   AltName: Full=Glutathione synthase {ECO:0000256|HAMAP-Rule:MF_00162};
GN   Name=gshB {ECO:0000256|HAMAP-Rule:MF_00162,
GN   ECO:0000313|EMBL:ABJ15374.1};
GN   OrderedLocusNames=PA14_05310 {ECO:0000313|EMBL:ABJ15374.1};
OS   Pseudomonas aeruginosa (strain UCBPP-PA14).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=208963 {ECO:0000313|EMBL:ABJ15374.1, ECO:0000313|Proteomes:UP000000653};
RN   [1] {ECO:0000313|EMBL:ABJ15374.1, ECO:0000313|Proteomes:UP000000653}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=UCBPP-PA14 {ECO:0000313|EMBL:ABJ15374.1,
RC   ECO:0000313|Proteomes:UP000000653};
RX   PubMed=17038190; DOI=10.1186/gb-2006-7-10-r90;
RA   Lee D.G., Urbach J.M., Wu G., Liberati N.T., Feinbaum R.L., Miyata S.,
RA   Diggins L.T., He J., Saucier M., Deziel E., Friedman L., Li L.,
RA   Grills G., Montgomery K., Kucherlapati R., Rahme L.G., Ausubel F.M.;
RT   "Genomic analysis reveals that Pseudomonas aeruginosa virulence is
RT   combinatorial.";
RL   Genome Biol. 7:R90.1-R90.14(2006).
CC   -!- CATALYTIC ACTIVITY: ATP + gamma-L-glutamyl-L-cysteine + glycine =
CC       ADP + phosphate + glutathione. {ECO:0000256|HAMAP-Rule:MF_00162}.
CC   -!- PATHWAY: Sulfur metabolism; glutathione biosynthesis; glutathione
CC       from L-cysteine and L-glutamate: step 2/2. {ECO:0000256|HAMAP-
CC       Rule:MF_00162}.
CC   -!- SIMILARITY: Belongs to the prokaryotic GSH synthase family.
CC       {ECO:0000256|HAMAP-Rule:MF_00162}.
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DR   EMBL; CP000438; ABJ15374.1; -; Genomic_DNA.
DR   RefSeq; WP_003100943.1; NC_008463.1.
DR   ProteinModelPortal; A0A0H2ZK52; -.
DR   SMR; A0A0H2ZK52; -.
DR   EnsemblBacteria; ABJ15374; ABJ15374; PA14_05310.
DR   KEGG; pau:PA14_05310; -.
DR   KO; K01920; -.
DR   OMA; WMRKDPP; -.
DR   UniPathway; UPA00142; UER00210.
DR   Proteomes; UP000000653; Chromosome.
DR   GO; GO:0005829; C:cytosol; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-HAMAP.
DR   GO; GO:0004363; F:glutathione synthase activity; IEA:UniProtKB-HAMAP.
DR   GO; GO:0046872; F:metal ion binding; IEA:InterPro.
DR   Gene3D; 3.30.1490.20; -; 1.
DR   HAMAP; MF_00162; GSH_S; 1.
DR   InterPro; IPR011761; ATP-grasp.
DR   InterPro; IPR013815; ATP_grasp_subdomain_1.
DR   InterPro; IPR006284; Glut_synth_pro.
DR   InterPro; IPR004218; GSHS_ATP-bd.
DR   InterPro; IPR004215; GSHS_N.
DR   InterPro; IPR016185; PreATP-grasp_dom.
DR   PANTHER; PTHR21621:SF10; PTHR21621:SF10; 1.
DR   Pfam; PF02955; GSH-S_ATP; 1.
DR   Pfam; PF02951; GSH-S_N; 1.
DR   SUPFAM; SSF52440; SSF52440; 1.
DR   TIGRFAMs; TIGR01380; glut_syn; 1.
DR   PROSITE; PS50975; ATP_GRASP; 1.
PE   3: Inferred from homology;
DR   PRODOM; A0A0H2ZK52.
DR   SWISS-2DPAGE; A0A0H2ZK52.
KW   ATP-binding {ECO:0000256|HAMAP-Rule:MF_00162, ECO:0000256|PROSITE-
KW   ProRule:PRU00409};
KW   Complete proteome {ECO:0000313|Proteomes:UP000000653};
KW   Glutathione biosynthesis {ECO:0000256|HAMAP-Rule:MF_00162};
KW   Ligase {ECO:0000256|HAMAP-Rule:MF_00162};
KW   Nucleotide-binding {ECO:0000256|HAMAP-Rule:MF_00162,
KW   ECO:0000256|PROSITE-ProRule:PRU00409}.
FT   DOMAIN      126    311       ATP-grasp. {ECO:0000259|PROSITE:PS50975}.
SQ   SEQUENCE   317 AA;  35705 MW;  543E960203929754 CRC64;
     MSVRLGIVMD PIARINFKKD SSLAMLLAAQ ARGWSLFYME QQDLYQKAGV ARGRMRPLKV
     FNDASRWFEL EAESDQPLHE LDVILMRKDP PFDNEFVYST YLLEQAERAG ALVVNRPQSL
     RDCNEKFFAT QFTQCTPPTM VSRRSDILRE FAAEHRDIIL KPLDGMGGSS IFRHREGDPN
     LSVILETLTQ HGSQQIMAQR YLPEIKDGDK RILMIDGEPV PYCLARIPAQ GETRGNLAAG
     GRGVAQPLSE RDRWIAAEVG PHLRERGLLF VGLDVIGDYL TEINVTSPTC IREIDQAFDT
     RIGDKLMDAI AAQLAAR
//

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