(data stored in SCRATCH zone)

SWISSPROT: A0KFH6_AERHH

ID   A0KFH6_AERHH            Unreviewed;       330 AA.
AC   A0KFH6;
DT   12-DEC-2006, integrated into UniProtKB/TrEMBL.
DT   12-DEC-2006, sequence version 1.
DT   08-MAY-2019, entry version 97.
DE   RecName: Full=Porphobilinogen deaminase {ECO:0000256|HAMAP-Rule:MF_00260};
DE            Short=PBG {ECO:0000256|HAMAP-Rule:MF_00260};
DE            EC=2.5.1.61 {ECO:0000256|HAMAP-Rule:MF_00260};
DE   AltName: Full=Hydroxymethylbilane synthase {ECO:0000256|HAMAP-Rule:MF_00260};
DE            Short=HMBS {ECO:0000256|HAMAP-Rule:MF_00260};
DE   AltName: Full=Pre-uroporphyrinogen synthase {ECO:0000256|HAMAP-Rule:MF_00260};
GN   Name=hemC {ECO:0000256|HAMAP-Rule:MF_00260,
GN   ECO:0000313|EMBL:ABK36245.1};
GN   OrderedLocusNames=AHA_0469 {ECO:0000313|EMBL:ABK36245.1};
OS   Aeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / DSM 30187 /
OS   JCM 1027 / KCTC 2358 / NCIMB 9240).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Aeromonadales;
OC   Aeromonadaceae; Aeromonas.
OX   NCBI_TaxID=380703 {ECO:0000313|EMBL:ABK36245.1, ECO:0000313|Proteomes:UP000000756};
RN   [1] {ECO:0000313|EMBL:ABK36245.1, ECO:0000313|Proteomes:UP000000756}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 7966 / DSM 30187 / JCM 1027 / KCTC 2358 / NCIMB 9240
RC   {ECO:0000313|Proteomes:UP000000756};
RX   PubMed=16980456; DOI=10.1128/JB.00621-06;
RA   Seshadri R., Joseph S.W., Chopra A.K., Sha J., Shaw J., Graf J.,
RA   Haft D., Wu M., Ren Q., Rosovitz M.J., Madupu R., Tallon L., Kim M.,
RA   Jin S., Vuong H., Stine O.C., Ali A., Horneman A.J., Heidelberg J.F.;
RT   "Genome sequence of Aeromonas hydrophila ATCC 7966T: jack of all
RT   trades.";
RL   J. Bacteriol. 188:8272-8282(2006).
CC   -!- FUNCTION: Tetrapolymerization of the monopyrrole PBG into the
CC       hydroxymethylbilane pre-uroporphyrinogen in several discrete
CC       steps. {ECO:0000256|HAMAP-Rule:MF_00260,
CC       ECO:0000256|SAAS:SAAS00129929}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H2O + 4 porphobilinogen = hydroxymethylbilane + 4 NH4(+);
CC         Xref=Rhea:RHEA:13185, ChEBI:CHEBI:15377, ChEBI:CHEBI:28938,
CC         ChEBI:CHEBI:57845, ChEBI:CHEBI:58126; EC=2.5.1.61;
CC         Evidence={ECO:0000256|HAMAP-Rule:MF_00260,
CC         ECO:0000256|SAAS:SAAS01118549};
CC   -!- COFACTOR:
CC       Name=dipyrromethane; Xref=ChEBI:CHEBI:60342;
CC         Evidence={ECO:0000256|HAMAP-Rule:MF_00260};
CC       Note=Binds 1 dipyrromethane group covalently. {ECO:0000256|HAMAP-
CC       Rule:MF_00260};
CC   -!- PATHWAY: Porphyrin-containing compound metabolism; protoporphyrin-
CC       IX biosynthesis; coproporphyrinogen-III from 5-aminolevulinate:
CC       step 2/4. {ECO:0000256|HAMAP-Rule:MF_00260,
CC       ECO:0000256|SAAS:SAAS00018329}.
CC   -!- SUBUNIT: Monomer. {ECO:0000256|HAMAP-Rule:MF_00260}.
CC   -!- MISCELLANEOUS: The porphobilinogen subunits are added to the
CC       dipyrromethane group. {ECO:0000256|HAMAP-Rule:MF_00260}.
CC   -!- SIMILARITY: Belongs to the HMBS family. {ECO:0000256|HAMAP-
CC       Rule:MF_00260, ECO:0000256|SAAS:SAAS00542451}.
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DR   EMBL; CP000462; ABK36245.1; -; Genomic_DNA.
DR   RefSeq; YP_855002.2; NC_008570.1.
DR   STRING; 380703.AHA_0469; -.
DR   EnsemblBacteria; ABK36245; ABK36245; AHA_0469.
DR   GeneID; 4488887; -.
DR   KEGG; aha:AHA_0469; -.
DR   PATRIC; fig|380703.7.peg.461; -.
DR   eggNOG; ENOG4105D6W; Bacteria.
DR   eggNOG; COG0181; LUCA.
DR   HOGENOM; HOG000228587; -.
DR   KO; K01749; -.
DR   OMA; LWQANHI; -.
DR   UniPathway; UPA00251; UER00319.
DR   Proteomes; UP000000756; Chromosome.
DR   GO; GO:0004418; F:hydroxymethylbilane synthase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0018160; P:peptidyl-pyrromethane cofactor linkage; IEA:InterPro.
DR   GO; GO:0006782; P:protoporphyrinogen IX biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.30.160.40; -; 1.
DR   HAMAP; MF_00260; Porphobil_deam; 1.
DR   InterPro; IPR000860; HemC.
DR   InterPro; IPR022419; Porphobilin_deaminase_cofac_BS.
DR   InterPro; IPR022417; Porphobilin_deaminase_N.
DR   InterPro; IPR022418; Porphobilinogen_deaminase_C.
DR   InterPro; IPR036803; Porphobilinogen_deaminase_C_sf.
DR   PANTHER; PTHR11557; PTHR11557; 1.
DR   Pfam; PF01379; Porphobil_deam; 1.
DR   Pfam; PF03900; Porphobil_deamC; 1.
DR   PIRSF; PIRSF001438; 4pyrrol_synth_OHMeBilane_synth; 1.
DR   PRINTS; PR00151; PORPHBDMNASE.
DR   SUPFAM; SSF54782; SSF54782; 1.
DR   TIGRFAMs; TIGR00212; hemC; 1.
DR   PROSITE; PS00533; PORPHOBILINOGEN_DEAM; 1.
PE   3: Inferred from homology;
DR   PRODOM; A0KFH6.
DR   SWISS-2DPAGE; A0KFH6.
KW   Complete proteome {ECO:0000313|Proteomes:UP000000756};
KW   Porphyrin biosynthesis {ECO:0000256|HAMAP-Rule:MF_00260,
KW   ECO:0000256|SAAS:SAAS00425415};
KW   Reference proteome {ECO:0000313|Proteomes:UP000000756};
KW   Transferase {ECO:0000256|HAMAP-Rule:MF_00260,
KW   ECO:0000256|SAAS:SAAS00425344, ECO:0000313|EMBL:ABK36245.1}.
FT   DOMAIN       27    233       Porphobil_deam. {ECO:0000259|Pfam:
FT                                PF01379}.
FT   DOMAIN      247    316       Porphobil_deamC. {ECO:0000259|Pfam:
FT                                PF03900}.
FT   MOD_RES     263    263       S-(dipyrrolylmethanemethyl)cysteine.
FT                                {ECO:0000256|HAMAP-Rule:MF_00260}.
SQ   SEQUENCE   330 AA;  36014 MW;  9EE9E423B44E6A62 CRC64;
     MKAPAKGGKI VFLLKMKQLI DMAARTLKIA TRKSPLALWQ ANFVKDRLEA LYPELQVELV
     PMSTQGDKIL DTPLAKVGGK GLFVKELETA MLEGRADIAV HSMKDVPVEF PEGLGLHTIC
     EREDPRDAFV SNRFGQIDEL PQGAVVGTSS LRRQCQLRAA RPDLVIRDLR GNVNTRLAKL
     DAGEYDAIIL AAAGLKRLEM AHRITAFIEP EQSLPANGQG AVGIECRLDD HELHALLAPL
     EHPETRIRVL TERAMNRALQ GGCQVPIGAY ALVQGEEVWL RGLVGSPDGS RVIRDEIRGP
     LADGEALGHT LAQRLLADGA DVILAEVYRA
//

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