(data stored in SCRATCH zone)

SWISSPROT: A0KFW5_AERHH

ID   A0KFW5_AERHH            Unreviewed;       355 AA.
AC   A0KFW5;
DT   12-DEC-2006, integrated into UniProtKB/TrEMBL.
DT   12-DEC-2006, sequence version 1.
DT   08-MAY-2019, entry version 105.
DE   RecName: Full=Sulfate/thiosulfate import ATP-binding protein CysA {ECO:0000256|HAMAP-Rule:MF_01701};
DE            EC=7.3.2.3 {ECO:0000256|HAMAP-Rule:MF_01701};
DE   AltName: Full=Sulfate-transporting ATPase {ECO:0000256|HAMAP-Rule:MF_01701};
GN   Name=cysA {ECO:0000256|HAMAP-Rule:MF_01701,
GN   ECO:0000313|EMBL:ABK39441.1};
GN   OrderedLocusNames=AHA_0608 {ECO:0000313|EMBL:ABK39441.1};
OS   Aeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / DSM 30187 /
OS   JCM 1027 / KCTC 2358 / NCIMB 9240).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Aeromonadales;
OC   Aeromonadaceae; Aeromonas.
OX   NCBI_TaxID=380703 {ECO:0000313|EMBL:ABK39441.1, ECO:0000313|Proteomes:UP000000756};
RN   [1] {ECO:0000313|EMBL:ABK39441.1, ECO:0000313|Proteomes:UP000000756}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 7966 / DSM 30187 / JCM 1027 / KCTC 2358 / NCIMB 9240
RC   {ECO:0000313|Proteomes:UP000000756};
RX   PubMed=16980456; DOI=10.1128/JB.00621-06;
RA   Seshadri R., Joseph S.W., Chopra A.K., Sha J., Shaw J., Graf J.,
RA   Haft D., Wu M., Ren Q., Rosovitz M.J., Madupu R., Tallon L., Kim M.,
RA   Jin S., Vuong H., Stine O.C., Ali A., Horneman A.J., Heidelberg J.F.;
RT   "Genome sequence of Aeromonas hydrophila ATCC 7966T: jack of all
RT   trades.";
RL   J. Bacteriol. 188:8272-8282(2006).
CC   -!- FUNCTION: Part of the ABC transporter complex CysAWTP involved in
CC       sulfate/thiosulfate import. Responsible for energy coupling to the
CC       transport system. {ECO:0000256|HAMAP-Rule:MF_01701,
CC       ECO:0000256|SAAS:SAAS00360330}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O + sulfate(out) = ADP + H(+) + phosphate +
CC         sulfate(in); Xref=Rhea:RHEA:10192, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:16189, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=7.3.2.3;
CC         Evidence={ECO:0000256|HAMAP-Rule:MF_01701,
CC         ECO:0000256|SAAS:SAAS01131877};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O + thiosulfate(out) = ADP + H(+) + phosphate +
CC         thiosulfate(in); Xref=Rhea:RHEA:29871, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:33542,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=7.3.2.3;
CC         Evidence={ECO:0000256|HAMAP-Rule:MF_01701,
CC         ECO:0000256|SAAS:SAAS01131887};
CC   -!- SUBUNIT: The complex is composed of two ATP-binding proteins
CC       (CysA), two transmembrane proteins (CysT and CysW) and a solute-
CC       binding protein (CysP). {ECO:0000256|HAMAP-Rule:MF_01701,
CC       ECO:0000256|SAAS:SAAS00360324}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000256|HAMAP-
CC       Rule:MF_01701}; Peripheral membrane protein {ECO:0000256|HAMAP-
CC       Rule:MF_01701}.
CC   -!- SIMILARITY: Belongs to the ABC transporter superfamily.
CC       Sulfate/tungstate importer (TC 3.A.1.6) family.
CC       {ECO:0000256|HAMAP-Rule:MF_01701, ECO:0000256|SAAS:SAAS00552311}.
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DR   EMBL; CP000462; ABK39441.1; -; Genomic_DNA.
DR   RefSeq; WP_011704575.1; NC_008570.1.
DR   RefSeq; YP_855147.1; NC_008570.1.
DR   STRING; 380703.AHA_0608; -.
DR   EnsemblBacteria; ABK39441; ABK39441; AHA_0608.
DR   GeneID; 4490155; -.
DR   KEGG; aha:AHA_0608; -.
DR   PATRIC; fig|380703.7.peg.605; -.
DR   eggNOG; ENOG4108IJ6; Bacteria.
DR   eggNOG; COG1118; LUCA.
DR   KO; K02045; -.
DR   OMA; MEVRVQN; -.
DR   BioCyc; AHYD380703:G1G7B-609-MONOMER; -.
DR   Proteomes; UP000000756; Chromosome.
DR   GO; GO:0043190; C:ATP-binding cassette (ABC) transporter complex; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0015419; F:ATPase-coupled sulfate transmembrane transporter activity; IEA:UniProtKB-UniRule.
DR   CDD; cd03296; ABC_CysA_sulfate_importer; 1.
DR   InterPro; IPR003593; AAA+_ATPase.
DR   InterPro; IPR014769; ABC_CysA_ATP-bd_C.
DR   InterPro; IPR003439; ABC_transporter-like.
DR   InterPro; IPR017871; ABC_transporter_CS.
DR   InterPro; IPR041193; CysA_C.
DR   InterPro; IPR008995; Mo/tungstate-bd_C_term_dom.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR005666; Sulph_transpt1.
DR   InterPro; IPR024765; TOBE-like.
DR   Pfam; PF00005; ABC_tran; 1.
DR   Pfam; PF17850; CysA_C_terminal; 1.
DR   Pfam; PF12857; TOBE_3; 1.
DR   SMART; SM00382; AAA; 1.
DR   SUPFAM; SSF50331; SSF50331; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR00968; 3a0106s01; 1.
DR   PROSITE; PS00211; ABC_TRANSPORTER_1; 1.
DR   PROSITE; PS50893; ABC_TRANSPORTER_2; 1.
DR   PROSITE; PS51237; CYSA; 1.
PE   3: Inferred from homology;
DR   PRODOM; A0KFW5.
DR   SWISS-2DPAGE; A0KFW5.
KW   ATP-binding {ECO:0000256|HAMAP-Rule:MF_01701, ECO:0000256|PROSITE-
KW   ProRule:PRU00434, ECO:0000256|SAAS:SAAS00237035,
KW   ECO:0000313|EMBL:ABK39441.1};
KW   Cell inner membrane {ECO:0000256|HAMAP-Rule:MF_01701};
KW   Cell membrane {ECO:0000256|HAMAP-Rule:MF_01701,
KW   ECO:0000256|SAAS:SAAS00437629};
KW   Complete proteome {ECO:0000313|Proteomes:UP000000756};
KW   Hydrolase {ECO:0000313|EMBL:ABK39441.1};
KW   Membrane {ECO:0000256|HAMAP-Rule:MF_01701,
KW   ECO:0000256|SAAS:SAAS00437643};
KW   Nucleotide-binding {ECO:0000256|HAMAP-Rule:MF_01701,
KW   ECO:0000256|PROSITE-ProRule:PRU00434, ECO:0000256|SAAS:SAAS00452314};
KW   Reference proteome {ECO:0000313|Proteomes:UP000000756};
KW   Sulfate transport {ECO:0000256|HAMAP-Rule:MF_01701,
KW   ECO:0000256|SAAS:SAAS00437635};
KW   Translocase {ECO:0000256|HAMAP-Rule:MF_01701,
KW   ECO:0000256|SAAS:SAAS01131881};
KW   Transport {ECO:0000256|HAMAP-Rule:MF_01701,
KW   ECO:0000256|SAAS:SAAS00237071}.
FT   DOMAIN        3    237       ABC transporter. {ECO:0000259|PROSITE:
FT                                PS50893}.
FT   DOMAIN      197    348       CYSA. {ECO:0000259|PROSITE:PS51237}.
FT   NP_BIND      35     42       ATP. {ECO:0000256|PROSITE-ProRule:
FT                                PRU00434}.
SQ   SEQUENCE   355 AA;  38703 MW;  E728693EFF346460 CRC64;
     MSIQVQGLNK HFNQYAALAD INLDFHDGEL VALLGPSGCG KTTLLRIIAG LEQADSGSVI
     IRGEDASDLH VRERNVGFVF QHYALFRHMT VFENVAFGLR VKPRSERPAE AAIRARVKEL
     LDLVQLSHVA ARYPTQLSGG QRQRVALARA LAVQPKVLLL DEPFGALDAQ VRKELRRWLR
     ELHDELHVTS LFVTHDQEEA LEVADRVVLM NAGKVEQIGT PAEVYDQPAS SFVHSFLGSV
     NLFRGRVAGQ GLGIGEQLLG GPISSQLAEG SAAVAYVRPH ELEILPADAP GGISATITRL
     LPMGPRIRVE LRGNGDTNGA HFEAELSKEA ARAFSPGQGV RLVAKQAQLY PDYQI
//

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