(data stored in SCRATCH zone)

SWISSPROT: A0KR25_AERHH

ID   A0KR25_AERHH            Unreviewed;       253 AA.
AC   A0KR25;
DT   12-DEC-2006, integrated into UniProtKB/TrEMBL.
DT   12-DEC-2006, sequence version 1.
DT   08-MAY-2019, entry version 74.
DE   RecName: Full=tRNA (guanosine(18)-2'-O)-methyltransferase {ECO:0000256|HAMAP-Rule:MF_02060};
DE            EC=2.1.1.34 {ECO:0000256|HAMAP-Rule:MF_02060};
DE   AltName: Full=tRNA [Gm18] methyltransferase {ECO:0000256|HAMAP-Rule:MF_02060};
GN   Name=trmH {ECO:0000256|HAMAP-Rule:MF_02060};
GN   OrderedLocusNames=AHA_0038 {ECO:0000313|EMBL:ABK39812.1};
OS   Aeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / DSM 30187 /
OS   JCM 1027 / KCTC 2358 / NCIMB 9240).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Aeromonadales;
OC   Aeromonadaceae; Aeromonas.
OX   NCBI_TaxID=380703 {ECO:0000313|EMBL:ABK39812.1, ECO:0000313|Proteomes:UP000000756};
RN   [1] {ECO:0000313|EMBL:ABK39812.1, ECO:0000313|Proteomes:UP000000756}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 7966 / DSM 30187 / JCM 1027 / KCTC 2358 / NCIMB 9240
RC   {ECO:0000313|Proteomes:UP000000756};
RX   PubMed=16980456; DOI=10.1128/JB.00621-06;
RA   Seshadri R., Joseph S.W., Chopra A.K., Sha J., Shaw J., Graf J.,
RA   Haft D., Wu M., Ren Q., Rosovitz M.J., Madupu R., Tallon L., Kim M.,
RA   Jin S., Vuong H., Stine O.C., Ali A., Horneman A.J., Heidelberg J.F.;
RT   "Genome sequence of Aeromonas hydrophila ATCC 7966T: jack of all
RT   trades.";
RL   J. Bacteriol. 188:8272-8282(2006).
CC   -!- FUNCTION: Catalyzes the 2'-O methylation of guanosine at position
CC       18 in tRNA. {ECO:0000256|HAMAP-Rule:MF_02060}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=guanosine(18) in tRNA + S-adenosyl-L-methionine = 2'-O-
CC         methylguanosine(18) in tRNA + H(+) + S-adenosyl-L-homocysteine;
CC         Xref=Rhea:RHEA:20077, Rhea:RHEA-COMP:10190, Rhea:RHEA-
CC         COMP:10192, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856,
CC         ChEBI:CHEBI:59789, ChEBI:CHEBI:74269, ChEBI:CHEBI:74445;
CC         EC=2.1.1.34; Evidence={ECO:0000256|HAMAP-Rule:MF_02060};
CC   -!- SIMILARITY: Belongs to the class IV-like SAM-binding
CC       methyltransferase superfamily. RNA methyltransferase TrmH family.
CC       {ECO:0000256|HAMAP-Rule:MF_02060}.
CC   -!- CAUTION: Lacks conserved residue(s) required for the propagation
CC       of feature annotation. {ECO:0000256|HAMAP-Rule:MF_02060}.
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DR   EMBL; CP000462; ABK39812.1; -; Genomic_DNA.
DR   RefSeq; WP_011704078.1; NC_008570.1.
DR   RefSeq; YP_854563.1; NC_008570.1.
DR   STRING; 380703.AHA_0038; -.
DR   EnsemblBacteria; ABK39812; ABK39812; AHA_0038.
DR   GeneID; 4489000; -.
DR   KEGG; aha:AHA_0038; -.
DR   PATRIC; fig|380703.7.peg.33; -.
DR   eggNOG; ENOG4105FE8; Bacteria.
DR   eggNOG; COG0566; LUCA.
DR   HOGENOM; HOG000285175; -.
DR   KO; K00556; -.
DR   OMA; TDRYQHV; -.
DR   BioCyc; AHYD380703:G1G7B-38-MONOMER; -.
DR   Proteomes; UP000000756; Chromosome.
DR   GO; GO:0009020; F:tRNA (guanosine-2'-O-)-methyltransferase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0000049; F:tRNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0002938; P:tRNA guanine ribose methylation; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.40.1280.10; -; 1.
DR   HAMAP; MF_02060; tRNA_methyltr_TrmH; 1.
DR   InterPro; IPR029028; Alpha/beta_knot_MTases.
DR   InterPro; IPR022724; rRNA_MeTrfase_SpoU_C.
DR   InterPro; IPR001537; SpoU_MeTrfase.
DR   InterPro; IPR033671; TrmH.
DR   InterPro; IPR029026; tRNA_m1G_MTases_N.
DR   Pfam; PF12105; SpoU_methylas_C; 1.
DR   Pfam; PF00588; SpoU_methylase; 1.
DR   SUPFAM; SSF75217; SSF75217; 1.
PE   3: Inferred from homology;
DR   PRODOM; A0KR25.
DR   SWISS-2DPAGE; A0KR25.
KW   Complete proteome {ECO:0000313|Proteomes:UP000000756};
KW   Methyltransferase {ECO:0000256|HAMAP-Rule:MF_02060,
KW   ECO:0000256|SAAS:SAAS00477853, ECO:0000313|EMBL:ABK39812.1};
KW   Reference proteome {ECO:0000313|Proteomes:UP000000756};
KW   RNA-binding {ECO:0000256|HAMAP-Rule:MF_02060};
KW   S-adenosyl-L-methionine {ECO:0000256|HAMAP-Rule:MF_02060};
KW   Transferase {ECO:0000256|HAMAP-Rule:MF_02060,
KW   ECO:0000256|SAAS:SAAS00477754, ECO:0000313|EMBL:ABK39812.1};
KW   tRNA processing {ECO:0000256|HAMAP-Rule:MF_02060};
KW   tRNA-binding {ECO:0000256|HAMAP-Rule:MF_02060}.
FT   DOMAIN       30    169       SpoU_methylase. {ECO:0000259|Pfam:
FT                                PF00588}.
FT   DOMAIN      173    227       SpoU_methylas_C. {ECO:0000259|Pfam:
FT                                PF12105}.
FT   BINDING     106    106       S-adenosyl-L-methionine.
FT                                {ECO:0000256|HAMAP-Rule:MF_02060}.
FT   BINDING     149    149       S-adenosyl-L-methionine; via amide
FT                                nitrogen and carbonyl oxygen.
FT                                {ECO:0000256|HAMAP-Rule:MF_02060}.
FT   BINDING     158    158       S-adenosyl-L-methionine; via amide
FT                                nitrogen. {ECO:0000256|HAMAP-Rule:
FT                                MF_02060}.
SQ   SEQUENCE   253 AA;  28502 MW;  AE4939E81653589B CRC64;
     MHTPHDIETD MTPERFKRIT DMLAQRQLDL TVCMEEVHKP HNLAAIVRTA DAIGIHRVHA
     VWPKTWIHKR KGTARGSQNW VDVKLHPDIG SAVAELKASG MQILATHLSD SSVDFRAIDY
     TKPTAILVGQ EKHGIGEEAL ALADHHIVIP MVGMVQSLNV SVAAAAILYE AQRQRELAGC
     YQRGCPLDLE EQNSILFEGG YPIYAQLCKE KEMPYPQLGP AGEILADEQW WQQMQLTRKG
     WAAQQEEDEW QDQ
//

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