(data stored in SCRATCH zone)

SWISSPROT: A1AZN2_PARDP

ID   A1AZN2_PARDP            Unreviewed;       359 AA.
AC   A1AZN2;
DT   23-JAN-2007, integrated into UniProtKB/TrEMBL.
DT   23-JAN-2007, sequence version 1.
DT   08-MAY-2019, entry version 90.
DE   RecName: Full=S-adenosylmethionine:tRNA ribosyltransferase-isomerase {ECO:0000256|HAMAP-Rule:MF_00113, ECO:0000256|SAAS:SAAS00958187};
DE            EC=2.4.99.17 {ECO:0000256|HAMAP-Rule:MF_00113, ECO:0000256|SAAS:SAAS00958171};
DE   AltName: Full=Queuosine biosynthesis protein QueA {ECO:0000256|HAMAP-Rule:MF_00113};
GN   Name=queA {ECO:0000256|HAMAP-Rule:MF_00113};
GN   OrderedLocusNames=Pden_0614 {ECO:0000313|EMBL:ABL68726.1};
OS   Paracoccus denitrificans (strain Pd 1222).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales;
OC   Rhodobacteraceae; Paracoccus.
OX   NCBI_TaxID=318586 {ECO:0000313|EMBL:ABL68726.1, ECO:0000313|Proteomes:UP000000361};
RN   [1] {ECO:0000313|Proteomes:UP000000361}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Pd 1222 {ECO:0000313|Proteomes:UP000000361};
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA   Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H.,
RA   Pitluck S., Munk A.C., Brettin T., Bruce D., Han C., Tapia R.,
RA   Gilna P., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N.,
RA   Lykidis A., Spiro S., Richardson D.J., Moir J.W.B., Ferguson S.J.,
RA   van Spanning R.J.M., Richardson P.;
RT   "Complete sequence of chromosome 1 of Paracoccus denitrificans
RT   PD1222.";
RL   Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Transfers and isomerizes the ribose moiety from AdoMet
CC       to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give
CC       epoxyqueuosine (oQ-tRNA). {ECO:0000256|HAMAP-Rule:MF_00113,
CC       ECO:0000256|SAAS:SAAS00958175}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=7-aminomethyl-7-carbaguanosine(34) in tRNA + S-adenosyl-
CC         L-methionine = adenine + epoxyqueuosine(34) in tRNA + H(+) + L-
CC         methionine; Xref=Rhea:RHEA:32155, Rhea:RHEA-COMP:10342,
CC         Rhea:RHEA-COMP:10346, ChEBI:CHEBI:15378, ChEBI:CHEBI:16708,
CC         ChEBI:CHEBI:57844, ChEBI:CHEBI:59789, ChEBI:CHEBI:82833,
CC         ChEBI:CHEBI:82834; EC=2.4.99.17; Evidence={ECO:0000256|HAMAP-
CC         Rule:MF_00113, ECO:0000256|SAAS:SAAS01116098};
CC   -!- PATHWAY: tRNA modification; tRNA-queuosine biosynthesis.
CC       {ECO:0000256|HAMAP-Rule:MF_00113, ECO:0000256|SAAS:SAAS00958180}.
CC   -!- SUBUNIT: Monomer. {ECO:0000256|HAMAP-Rule:MF_00113,
CC       ECO:0000256|SAAS:SAAS00958162}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00113,
CC       ECO:0000256|SAAS:SAAS00958183}.
CC   -!- SIMILARITY: Belongs to the QueA family. {ECO:0000256|HAMAP-
CC       Rule:MF_00113, ECO:0000256|SAAS:SAAS00958161}.
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DR   EMBL; CP000489; ABL68726.1; -; Genomic_DNA.
DR   STRING; 318586.Pden_0614; -.
DR   PRIDE; A1AZN2; -.
DR   EnsemblBacteria; ABL68726; ABL68726; Pden_0614.
DR   KEGG; pde:Pden_0614; -.
DR   eggNOG; ENOG4105E2N; Bacteria.
DR   eggNOG; COG0809; LUCA.
DR   HOGENOM; HOG000004401; -.
DR   KO; K07568; -.
DR   OMA; YSYGDGM; -.
DR   UniPathway; UPA00392; -.
DR   Proteomes; UP000000361; Chromosome 1.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016853; F:isomerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0016740; F:transferase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0008616; P:queuosine biosynthetic process; IEA:UniProtKB-UniRule.
DR   Gene3D; 2.40.10.240; -; 1.
DR   Gene3D; 3.40.1780.10; -; 1.
DR   HAMAP; MF_00113; QueA; 1.
DR   InterPro; IPR003699; QueA.
DR   InterPro; IPR042118; QueA_dom1.
DR   InterPro; IPR042119; QueA_dom2.
DR   InterPro; IPR036100; QueA_sf.
DR   PANTHER; PTHR30307; PTHR30307; 1.
DR   Pfam; PF02547; Queuosine_synth; 1.
DR   SUPFAM; SSF111337; SSF111337; 1.
DR   TIGRFAMs; TIGR00113; queA; 1.
PE   3: Inferred from homology;
DR   PRODOM; A1AZN2.
DR   SWISS-2DPAGE; A1AZN2.
KW   Complete proteome {ECO:0000313|Proteomes:UP000000361};
KW   Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00113,
KW   ECO:0000256|SAAS:SAAS00958179};
KW   Queuosine biosynthesis {ECO:0000256|HAMAP-Rule:MF_00113,
KW   ECO:0000256|SAAS:SAAS00958170};
KW   Reference proteome {ECO:0000313|Proteomes:UP000000361};
KW   S-adenosyl-L-methionine {ECO:0000256|HAMAP-Rule:MF_00113,
KW   ECO:0000256|SAAS:SAAS00958172};
KW   Transferase {ECO:0000256|HAMAP-Rule:MF_00113,
KW   ECO:0000256|SAAS:SAAS00958166}.
SQ   SEQUENCE   359 AA;  38718 MW;  F3C6498EFFAC9ED7 CRC64;
     MARTEPKPMK LDDFDFHLPE TLIATRPARP RSSARLLLAQ GDSIRDLHVR DLADLLGSGD
     LLVLNDTKVI PARLNGTRAR QTPQGEAVAK VEVTLLEPVA HGWKALAKPL RKLKPGEVIR
     FGSALSAEVA EIAGGELTLR FDATGEAFDA ALAQVGAMPL PPYIAALRAP DEADRQDYQT
     VWARNSGAVA APTASLHFDG ELLDGLAARG VRFTHVTLHV GAGTFLPVKV EDVTTHKMHG
     EWGEVTPEAA AAINATRAAG GRVIPVGTTA LRLIESAAAE DGTVHPFQGV TEIFIYPGYR
     FRVTDALMTN FHLPKSTLLM LVSALMGQDR IRGIYRHAVE SGYRFFSYGD ASLLIPKDP
//

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