(data stored in SCRATCH zone)

SWISSPROT: A4F5Q3_SACEN

ID   A4F5Q3_SACEN            Unreviewed;       526 AA.
AC   A4F5Q3;
DT   17-APR-2007, integrated into UniProtKB/TrEMBL.
DT   17-APR-2007, sequence version 1.
DT   08-MAY-2019, entry version 60.
DE   SubName: Full=2-nitropropane dioxygenase, NPD {ECO:0000313|EMBL:CAL99377.1};
DE   SubName: Full=PfaD family protein {ECO:0000313|EMBL:PFG93177.1};
GN   OrderedLocusNames=SACE_0024 {ECO:0000313|EMBL:CAL99377.1};
GN   ORFNames=A8924_0407 {ECO:0000313|EMBL:PFG93177.1};
OS   Saccharopolyspora erythraea (strain ATCC 11635 / DSM 40517 / JCM 4748
OS   / NBRC 13426 / NCIMB 8594 / NRRL 2338).
OC   Bacteria; Actinobacteria; Pseudonocardiales; Pseudonocardiaceae;
OC   Saccharopolyspora.
OX   NCBI_TaxID=405948 {ECO:0000313|EMBL:CAL99377.1, ECO:0000313|Proteomes:UP000006728};
RN   [1] {ECO:0000313|EMBL:CAL99377.1, ECO:0000313|Proteomes:UP000006728}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 11635 / DSM 40517 / JCM 4748 / NBRC 13426 / NCIMB 8594 /
RC   NRRL 2338 {ECO:0000313|Proteomes:UP000006728}, and NRRL 2338
RC   {ECO:0000313|EMBL:CAL99377.1};
RX   PubMed=17369815; DOI=10.1038/nbt1297;
RA   Oliynyk M., Samborskyy M., Lester J.B., Mironenko T., Scott N.,
RA   Dickens S., Haydock S.F., Leadlay P.F.;
RT   "Complete genome sequence of the erythromycin-producing bacterium
RT   Saccharopolyspora erythraea NRRL23338.";
RL   Nat. Biotechnol. 25:447-453(2007).
RN   [2] {ECO:0000313|EMBL:PFG93177.1, ECO:0000313|Proteomes:UP000225825}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 40517 {ECO:0000313|EMBL:PFG93177.1,
RC   ECO:0000313|Proteomes:UP000225825};
RA   Klenk H.-P.;
RT   "Sequencing the genomes of 1000 actinobacteria strains.";
RL   Submitted (SEP-2017) to the EMBL/GenBank/DDBJ databases.
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DR   EMBL; AM420293; CAL99377.1; -; Genomic_DNA.
DR   EMBL; PDBV01000001; PFG93177.1; -; Genomic_DNA.
DR   RefSeq; WP_009945666.1; NZ_PDBV01000001.1.
DR   STRING; 405948.SACE_0024; -.
DR   EnsemblBacteria; CAL99377; CAL99377; SACE_0024.
DR   KEGG; sen:SACE_0024; -.
DR   eggNOG; ENOG4107QKG; Bacteria.
DR   eggNOG; COG2070; LUCA.
DR   HOGENOM; HOG000294495; -.
DR   OMA; KRGTMFP; -.
DR   OrthoDB; 1123553at2; -.
DR   BioCyc; SERY405948:SACE_RS00115-MONOMER; -.
DR   Proteomes; UP000006728; Chromosome.
DR   Proteomes; UP000225825; Unassembled WGS sequence.
DR   GO; GO:0051213; F:dioxygenase activity; IEA:UniProtKB-KW.
DR   GO; GO:0018580; F:nitronate monooxygenase activity; IEA:InterPro.
DR   Gene3D; 3.20.20.70; -; 1.
DR   InterPro; IPR013785; Aldolase_TIM.
DR   InterPro; IPR004136; NMO.
DR   InterPro; IPR014179; PfaD_fam.
DR   InterPro; IPR040981; PfaD_N.
DR   Pfam; PF03060; NMO; 1.
DR   Pfam; PF18328; PfaD_N; 1.
DR   TIGRFAMs; TIGR02814; pfaD_fam; 1.
PE   4: Predicted;
DR   PRODOM; A4F5Q3.
DR   SWISS-2DPAGE; A4F5Q3.
KW   Complete proteome {ECO:0000313|Proteomes:UP000006728,
KW   ECO:0000313|Proteomes:UP000225825};
KW   Dioxygenase {ECO:0000313|EMBL:CAL99377.1};
KW   Oxidoreductase {ECO:0000313|EMBL:CAL99377.1};
KW   Reference proteome {ECO:0000313|Proteomes:UP000006728}.
FT   DOMAIN       11     47       PfaD_N. {ECO:0000259|Pfam:PF18328}.
FT   DOMAIN       84    346       NMO. {ECO:0000259|Pfam:PF03060}.
SQ   SEQUENCE   526 AA;  57098 MW;  3CE53774A0901E9C CRC64;
     MTATTAPDAA PAPVRTDPAG VYQALRRLDR PCYVVDTGAG YGASNELPAE QDQVLAAVGP
     LPPERLGSTA FGDRHGTRYA YMAGSMAGGV ASEDLVIAMA RAGLLASFGS AGLLPERIEK
     ALVRFAEEIP DRPYAVNLIH SPSEEGLERG AVELYLRHGV RCVEASAFMD VTPHLVRYRV
     AGLERGAQGA PLARNRLIAK VSRPEVAEKF LHPAPPSMVD ALAADGLVTA EQAELARHVP
     LADDVTAEAD SGGHTDRRPM PALLPTILRQ RDEVRRRHRY RERVGIGAAG GIGTPTAATA
     AFAMGADYVV TGSVNQSCLE AGTSPAVRGL LAGAGIADCE MAPAADMFEL GVQLQVLKRG
     TLFPMRAKQL YELYRDHDGV EALPEVERAR LEKSVLRRPV EQVWEEVVGY FRRRDPDQLE
     RAAADPRRRM ALIFRWYLGM ASRWANTGEA GREQDYQIWC GPAMGAFNEW VRGSHLAEPD
     NRAVAEVAWQ LLRGAAFTSR VHQLQLCGVR LPASCAEYRP EAGDQR
//

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