(data stored in SCRATCH zone)

SWISSPROT: A4F6I1_SACEN

ID   A4F6I1_SACEN            Unreviewed;       594 AA.
AC   A4F6I1;
DT   17-APR-2007, integrated into UniProtKB/TrEMBL.
DT   17-APR-2007, sequence version 1.
DT   11-DEC-2019, entry version 74.
DE   RecName: Full=Beta-galactosidase {ECO:0000256|RuleBase:RU000675};
DE            EC=3.2.1.23 {ECO:0000256|RuleBase:RU000675};
GN   Name=bga {ECO:0000313|EMBL:CAL99655.1};
GN   OrderedLocusNames=SACE_0306 {ECO:0000313|EMBL:CAL99655.1};
GN   ORFNames=A8924_0700 {ECO:0000313|EMBL:PFG93458.1};
OS   Saccharopolyspora erythraea (strain ATCC 11635 / DSM 40517 / JCM 4748 /
OS   NBRC 13426 / NCIMB 8594 / NRRL 2338).
OC   Bacteria; Actinobacteria; Pseudonocardiales; Pseudonocardiaceae;
OC   Saccharopolyspora.
OX   NCBI_TaxID=405948 {ECO:0000313|EMBL:CAL99655.1, ECO:0000313|Proteomes:UP000006728};
RN   [1] {ECO:0000313|EMBL:CAL99655.1, ECO:0000313|Proteomes:UP000006728}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 11635 / DSM 40517 / JCM 4748 / NBRC 13426 / NCIMB 8594 /
RC   NRRL 2338 {ECO:0000313|Proteomes:UP000006728}, and NRRL 2338
RC   {ECO:0000313|EMBL:CAL99655.1};
RX   PubMed=17369815; DOI=10.1038/nbt1297;
RA   Oliynyk M., Samborskyy M., Lester J.B., Mironenko T., Scott N., Dickens S.,
RA   Haydock S.F., Leadlay P.F.;
RT   "Complete genome sequence of the erythromycin-producing bacterium
RT   Saccharopolyspora erythraea NRRL23338.";
RL   Nat. Biotechnol. 25:447-453(2007).
RN   [2] {ECO:0000313|EMBL:PFG93458.1, ECO:0000313|Proteomes:UP000225825}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 40517 {ECO:0000313|EMBL:PFG93458.1,
RC   ECO:0000313|Proteomes:UP000225825};
RA   Klenk H.-P.;
RT   "Sequencing the genomes of 1000 actinobacteria strains.";
RL   Submitted (SEP-2017) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Hydrolysis of terminal non-reducing beta-D-galactose residues
CC         in beta-D-galactosides.; EC=3.2.1.23;
CC         Evidence={ECO:0000256|RuleBase:RU000675};
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 35 family.
CC       {ECO:0000256|RuleBase:RU003679}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AM420293; CAL99655.1; -; Genomic_DNA.
DR   EMBL; PDBV01000001; PFG93458.1; -; Genomic_DNA.
DR   RefSeq; WP_009946943.1; NZ_PDBV01000001.1.
DR   STRING; 405948.SACE_0306; -.
DR   CAZy; GH35; Glycoside Hydrolase Family 35.
DR   EnsemblBacteria; CAL99655; CAL99655; SACE_0306.
DR   KEGG; sen:SACE_0306; -.
DR   eggNOG; ENOG4105D57; Bacteria.
DR   eggNOG; COG1874; LUCA.
DR   HOGENOM; HOG000221607; -.
DR   KO; K12308; -.
DR   OMA; ACDFSYM; -.
DR   OrthoDB; 831762at2; -.
DR   BioCyc; SERY405948:SACE_RS01495-MONOMER; -.
DR   Proteomes; UP000006728; Chromosome.
DR   Proteomes; UP000225825; Unassembled WGS sequence.
DR   GO; GO:0004565; F:beta-galactosidase activity; IEA:UniProtKB-EC.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   Gene3D; 2.60.120.260; -; 2.
DR   InterPro; IPR026283; B-gal_1-like.
DR   InterPro; IPR008979; Galactose-bd-like_sf.
DR   InterPro; IPR031330; Gly_Hdrlase_35_cat.
DR   InterPro; IPR019801; Glyco_hydro_35_CS.
DR   InterPro; IPR001944; Glycoside_Hdrlase_35.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   PANTHER; PTHR23421; PTHR23421; 1.
DR   Pfam; PF01301; Glyco_hydro_35; 1.
DR   PIRSF; PIRSF006336; B-gal; 1.
DR   PRINTS; PR00742; GLHYDRLASE35.
DR   SUPFAM; SSF49785; SSF49785; 1.
DR   SUPFAM; SSF51445; SSF51445; 1.
DR   PROSITE; PS01182; GLYCOSYL_HYDROL_F35; 1.
PE   3: Inferred from homology;
DR   PRODOM; A4F6I1.
DR   SWISS-2DPAGE; A4F6I1.
KW   Glycosidase {ECO:0000256|RuleBase:RU000675, ECO:0000313|EMBL:CAL99655.1};
KW   Hydrolase {ECO:0000256|RuleBase:RU000675, ECO:0000313|EMBL:CAL99655.1};
KW   Reference proteome {ECO:0000313|Proteomes:UP000006728}.
FT   DOMAIN          23..339
FT                   /note="Glyco_hydro_35"
FT                   /evidence="ECO:0000259|Pfam:PF01301"
FT   ACT_SITE        171
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR006336-1"
FT   ACT_SITE        247
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR006336-1"
SQ   SEQUENCE   594 AA;  65648 MW;  51203723A3E9BD48 CRC64;
     MGGTNRVLAQ AGKPAGLTVR GNEFLLDGEP FRIIAGEMHY FRTHPDQWRN RLDRMRALGL
     NSVDTYVAWN FHEPRRGEVD FTGWRDVVRF VETAAEAGLK VIIRPGPYIC AEWDFGGLPA
     WLLESGNPPL RCSDPAYTEL TLRWFDELLP RLAPLQATRG GPVLAFQVEN EYGSYGNDQT
     HLEQLRAGML ERGIDSLLFC SNGPSDYMLR GGNLPDTLAT VNFAGDPTAP FEALREYQPE
     GPLWCTEFWD GWFDHWGEEH HTTDPVETAG HVDRMLAAGA SVSLYMAVGG TNFGWWAGAN
     YDTSKDQYQP TITSYDYDSP IGEAGELTEK FQRIREAIAR HAPVPEPPLP APPPRLRPQT
     ARPEATTGLL ESLDVLSSPV RSATPAHMEK FGQPYGLIHY RTTVQGPRQT AGLRVSGLAD
     RALVFSDGKY LGALDRNTPD LTIDVAVPGR TGRLDLLVDT GGRVNFGHQL SDPKGITDRV
     LLGMQALHGW EIRPLPLEDL SGLRFGGGTS AGPVFHRARM SIAEPADGFL ALPGWEKGMV
     WLNGFLLGRY WSVGPQVTLY APSPLWKQGE NEIVVLEMHE AGQDIELRSE PDLG
//

If you have problems or comments...

PBIL Back to PBIL home page