(data stored in SCRATCH zone)

SWISSPROT: A4F7N8_SACEN

ID   A4F7N8_SACEN            Unreviewed;      3545 AA.
AC   A4F7N8;
DT   17-APR-2007, integrated into UniProtKB/TrEMBL.
DT   17-APR-2007, sequence version 1.
DT   08-MAY-2019, entry version 88.
DE   SubName: Full=EryAI Erythromycin polyketide synthase modules 1 and 2 {ECO:0000313|EMBL:CAM00062.1};
DE   SubName: Full=Erythronolide synthase {ECO:0000313|EMBL:PFG93864.1};
GN   Name=eryAI {ECO:0000313|EMBL:CAM00062.1};
GN   OrderedLocusNames=SACE_0721 {ECO:0000313|EMBL:CAM00062.1};
GN   ORFNames=A8924_1117 {ECO:0000313|EMBL:PFG93864.1};
OS   Saccharopolyspora erythraea (strain ATCC 11635 / DSM 40517 / JCM 4748
OS   / NBRC 13426 / NCIMB 8594 / NRRL 2338).
OC   Bacteria; Actinobacteria; Pseudonocardiales; Pseudonocardiaceae;
OC   Saccharopolyspora.
OX   NCBI_TaxID=405948 {ECO:0000313|EMBL:CAM00062.1, ECO:0000313|Proteomes:UP000006728};
RN   [1] {ECO:0000313|EMBL:CAM00062.1, ECO:0000313|Proteomes:UP000006728}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 11635 / DSM 40517 / JCM 4748 / NBRC 13426 / NCIMB 8594 /
RC   NRRL 2338 {ECO:0000313|Proteomes:UP000006728}, and NRRL 2338
RC   {ECO:0000313|EMBL:CAM00062.1};
RX   PubMed=17369815; DOI=10.1038/nbt1297;
RA   Oliynyk M., Samborskyy M., Lester J.B., Mironenko T., Scott N.,
RA   Dickens S., Haydock S.F., Leadlay P.F.;
RT   "Complete genome sequence of the erythromycin-producing bacterium
RT   Saccharopolyspora erythraea NRRL23338.";
RL   Nat. Biotechnol. 25:447-453(2007).
RN   [2] {ECO:0000313|EMBL:PFG93864.1, ECO:0000313|Proteomes:UP000225825}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 40517 {ECO:0000313|EMBL:PFG93864.1,
RC   ECO:0000313|Proteomes:UP000225825};
RA   Klenk H.-P.;
RT   "Sequencing the genomes of 1000 actinobacteria strains.";
RL   Submitted (SEP-2017) to the EMBL/GenBank/DDBJ databases.
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DR   EMBL; AM420293; CAM00062.1; -; Genomic_DNA.
DR   EMBL; PDBV01000001; PFG93864.1; -; Genomic_DNA.
DR   STRING; 405948.SACE_0721; -.
DR   EnsemblBacteria; CAM00062; CAM00062; SACE_0721.
DR   KEGG; sen:SACE_0721; -.
DR   eggNOG; ENOG4107EEY; Bacteria.
DR   eggNOG; COG3321; LUCA.
DR   HOGENOM; HOG000046292; -.
DR   KO; K10817; -.
DR   Proteomes; UP000006728; Chromosome.
DR   Proteomes; UP000225825; Unassembled WGS sequence.
DR   GO; GO:0031177; F:phosphopantetheine binding; IEA:InterPro.
DR   GO; GO:0016740; F:transferase activity; IEA:InterPro.
DR   Gene3D; 1.10.1200.10; -; 3.
DR   Gene3D; 3.40.366.10; -; 3.
DR   Gene3D; 3.40.47.10; -; 2.
DR   InterPro; IPR001227; Ac_transferase_dom_sf.
DR   InterPro; IPR036736; ACP-like_sf.
DR   InterPro; IPR014043; Acyl_transferase.
DR   InterPro; IPR016035; Acyl_Trfase/lysoPLipase.
DR   InterPro; IPR032821; KAsynt_C_assoc.
DR   InterPro; IPR018201; Ketoacyl_synth_AS.
DR   InterPro; IPR014031; Ketoacyl_synth_C.
DR   InterPro; IPR014030; Ketoacyl_synth_N.
DR   InterPro; IPR016036; Malonyl_transacylase_ACP-bd.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   InterPro; IPR020801; PKS_acyl_transferase.
DR   InterPro; IPR020841; PKS_Beta-ketoAc_synthase_dom.
DR   InterPro; IPR013968; PKS_KR.
DR   InterPro; IPR020806; PKS_PP-bd.
DR   InterPro; IPR009081; PP-bd_ACP.
DR   InterPro; IPR006162; Ppantetheine_attach_site.
DR   InterPro; IPR016039; Thiolase-like.
DR   Pfam; PF00698; Acyl_transf_1; 3.
DR   Pfam; PF16197; KAsynt_C_assoc; 2.
DR   Pfam; PF00109; ketoacyl-synt; 2.
DR   Pfam; PF02801; Ketoacyl-synt_C; 2.
DR   Pfam; PF08659; KR; 2.
DR   Pfam; PF00550; PP-binding; 3.
DR   SMART; SM00827; PKS_AT; 3.
DR   SMART; SM00825; PKS_KS; 2.
DR   SMART; SM00823; PKS_PP; 3.
DR   SUPFAM; SSF47336; SSF47336; 3.
DR   SUPFAM; SSF51735; SSF51735; 4.
DR   SUPFAM; SSF52151; SSF52151; 3.
DR   SUPFAM; SSF53901; SSF53901; 2.
DR   SUPFAM; SSF55048; SSF55048; 3.
DR   PROSITE; PS00606; B_KETOACYL_SYNTHASE; 2.
DR   PROSITE; PS50075; CARRIER; 3.
DR   PROSITE; PS00012; PHOSPHOPANTETHEINE; 3.
PE   4: Predicted;
DR   PRODOM; A4F7N8.
DR   SWISS-2DPAGE; A4F7N8.
KW   Complete proteome {ECO:0000313|Proteomes:UP000006728,
KW   ECO:0000313|Proteomes:UP000225825};
KW   Phosphopantetheine {ECO:0000256|PROSITE-ProRule:PRU00258};
KW   Reference proteome {ECO:0000313|Proteomes:UP000006728}.
FT   DOMAIN      465    540       Carrier. {ECO:0000259|PROSITE:PS50075}.
FT   DOMAIN     1939   2014       Carrier. {ECO:0000259|PROSITE:PS50075}.
FT   DOMAIN     3383   3461       Carrier. {ECO:0000259|PROSITE:PS50075}.
FT   MOD_RES     500    500       O-(pantetheine 4'-phosphoryl)serine.
FT                                {ECO:0000256|PROSITE-ProRule:PRU00258}.
FT   MOD_RES    1974   1974       O-(pantetheine 4'-phosphoryl)serine.
FT                                {ECO:0000256|PROSITE-ProRule:PRU00258}.
FT   MOD_RES    3421   3421       O-(pantetheine 4'-phosphoryl)serine.
FT                                {ECO:0000256|PROSITE-ProRule:PRU00258}.
SQ   SEQUENCE   3545 AA;  370660 MW;  4B4E7C2B1346BF05 CRC64;
     MADLSKLSDS RTAQPGRIVR PWPLSGCNES ALRARARQLR AHLDRFPDAG VEGVGAALAH
     DEQADAGPHR AVVVASSTSE LLDGLAAVAD GRPHASVVRG VARPSAPVVF VFPGQGAQWA
     GMAGELLGES RVFAAAMDAC ARAFEPVTDW TLAQVLDSPE QSRRVEVVQP ALFAVQTSLA
     ALWRSFGVTP DAVVGHSIGE LAAAHVCGAA GAADAARAAA LWSREMIPLV GNGDMAAVAL
     SADEIEPRIA RWDDDVVLAG VNGPRSVLLT GSPEPVARRV QELSAEGVRA QVINVSMAAH
     SAQVDDIAEG MRSALAWFAP GGSEVPFYAS LTGGAVDTRE LVADYWRRSF RLPVRFDEAI
     RSALEVGPGT FVEASPHPVL AAALQQTLDA EGSSAAVVPT LQRGQGGMRR FLLAAAQAFT
     GGVAVDWTAA YDDVGAEPGS LPEFAPAEEE DEPAESGVDW NAPPHVLRER LLAVVNGETA
     ALAGREADAE ATFRELGLDS VLAAQLRAKV SAAIGREVNI ALLYDHPTPR ALAEALAAGT
     EVAQRETRAR TNEAAPGEPV AVVAMACRLP GGVSTPEEFW ELLSEGRDAV AGLPTDRGWD
     LDSLFHPDPT RSGTAHQRGG GFLTEATAFD PAFFGMSPRE ALAVDPQQRL MLELSWEVLE
     RAGIPPTSLQ ASPTGVFVGL IPQEYGPRLA EGGEGVEGYL MTGTTTSVAS GRIAYTLGLE
     GPAISVDTAC SSSLVAVHLA CQSLRRGESS LAMAGGVTVM PTPGMLVDFS RMNSLAPDGR
     CKAFSAGANG FGMAEGAGML LLERLSDARR NGHPVLAVLR GTAVNSDGAS NGLSAPNGRA
     QVRVIQQALA ESGLGPADID AVEAHGTGTR LGDPIEARAL FEAYGRDREQ PLHLGSVKSN
     LGHTQAAAGV AGVIKMVLAM RAGTLPRTLH ASERSKEIDW SSGAISLLDE PEPWPAGARP
     RRAGVSSFGI SGTNAHAIIE EAPQVVEGER VEAGDVVAPW VLSASSAEGL RAQAARLAAH
     LREHPGQDPR DIAYSLATGR AALPHRAAFA PVDESAALRV LDGLATGNAD GAAVGTSRAQ
     QRAVFVFPGQ GWQWAGMAVD LLDTSPVFAA ALRECADALE PHLDFEVIPF LRAEAARREQ
     DAALSTERVD VVQPVMFAVM VSLASMWRAH GVEPAAVIGH SQGEIAAACV AGALSLDDAA
     RVVALRSRVI ATMPGNKGMA SIAAPAGEVR ARIGDRVEIA AVNGPRSVVV AGDSDELDRL
     VASCTTECIR AKRLAVDYAS HSSHVETIRD ALHAELGEDF HPLPGFVPFF STVTGRWTQP
     DELDAGYWYR NLRRTVRFAD AVRALAEQGY RTFLEVSAHP ILTAAIEEIG DGSGADLSAI
     HSLRRGDGSL ADFGEALSRA FAAGVAVDWE SVHLGTGARR VPLPTYPFQR ERVWLEPKPV
     ARRSTEVDEV SALRYRIEWR PTGAGEPARL DGTWLVAKYA GTADETSTAA REALESAGAR
     VRELVVDARC GRDELAERLR SVGEVAGVLS LLAVDEAEPE EAPLALASLA DTLSLVQAMV
     SAELGCPLWT VTESAVATGP FERVRNAAHG ALWGVGRVIA LENPAVWGGL VDVPAGSVAE
     LARHLAAVVS GGAGEDQLAL RADGVYGRRW VRAAAPATDD EWKPTGTVLV TGGTGGVGGQ
     IARWLARRGA PHLLLVSRSG PDADGAGELV AELEALGART TVAACDVTDR ESVRELLGGI
     GDDVPLSAVF HAAATLDDGT VDTLTGERIE RASRAKVLGA RNLHELTREL DLTAFVLFSS
     FASAFGAPGL GGYAPGNAYL DGLAQQRRSD GLPATAVAWG TWAGSGMAEG PVADRFRRHG
     VIEMPPETAC RALQNALDRA EVCPIVIDVR WDRFLLAYTA QRPTRLFDEI DDARRAAPQA
     AAEPRVGALA SLPAPEREKA LFELVRSHAA AVLGHASAER VPADQAFAEL GVDSLSALEL
     RNRLGAATGV RLPTTTVFDH PDVRTLAAHL AAELGGATGA EQAAPATTAP VDEPIAIVGM
     ACRLPGEVDS PERLWELITS GRDSAAEVPD DRGWVPDELM ASDAAGTRRA HGNFMAGAGD
     FDAAFFGISP REALAMDPQQ RQALETTWEA LESAGIPPET LRGSDTGVFV GMSHQGYATG
     RPRPEDGVDG YLLTGNTASV ASGRIAYVLG LEGPALTVDT ACSSSLVALH TACGSLRDGD
     CGLAVAGGVS VMAGPEVFTE FSRQGALSPD GRCKPFSDEA DGFGLGEGSA FVVLQRLSDA
     RREGRRVLGV VAGSAVNQDG ASNGLSAPSG VAQQRVIRRA WARAGITGAD VAVVEAHGTG
     TRLGDPVEAS ALLATYGKSR GSSGPVLLGS VKSNIGHAQA AAGVAGVIKV LLGLERGVVP
     PMLCRGERSG LIDWSSGEIE LADGVREWSP AADGVRRAGV SAFGVSGTNA HVIIAEPPEP
     EPVPQPRRML PATGVVPVVL SARTGAALRA QAGRLADHLA AHPGIAPADV SWTMARARQH
     FEERAAVLAA DTAEAVHRLR AVADGAVVPG VVTGSASDGG SVFVFPGQGA QWEGMARELL
     PVPVFAESIA ECDAVLSEVA GFSVSEVLEP RPDAPSLERV DVVQPVLFAV MVSLARLWRA
     CGAVPSAVIG HSQGEIAAAV VAGALSLEDG MRVVARRSRA VRAVAGRGSM LSVRGGRSDV
     EKLLADDSWT GRLEVAAVNG PDAVVVAGDA QAAREFLEYC EGVGIRARAI PVDYASHTAH
     VEPVRDELVQ ALAGITPRRA EVPFFSTLTG DFLDGTELDA GYWYRNLRHP VEFHSAVQAL
     TDQGYATFIE VSPHPVLASS VQETLDDAES DAAVLGTLER DAGDADRFLT ALADAHTRGV
     AVDWEAVLGR AGLVDLPGYP FQGKRFWLLP DRTTPRDELD GWFYRVDWTE VPRSEPAALR
     GRWLVVVPEG HEEDGWTVEV RSALAEAGAE PEVTRGVGGL VGDCAGVVSL LALEGDGAVQ
     TLVLVRELDA EGIDAPLWTV TFGAVDAGSP VARPDQAKLW GLGQVASLER GPRWTGLVDL
     PHMPDPELRG RLTAVLAGSE DQVAVRADAV RARRLSPAHV TATSEYAVPG GTILVTGGTA
     GLGAEVARWL AGRGAEHLAL VSRRGPDTEG VGDLTAELTR LGARVSVHAC DVSSREPVRE
     LVHGLIEQGD VVRGVVHAAG LPQQVAINDM DEAAFDEVVA AKAGGAVHLD ELCSDAELFL
     LFSSGAGVWG SARQGAYAAG NAFLDAFARH RRGRGLPATS VAWGLWAAGG MTGDEEAVSF
     LRERGVRAMP VPRALAALDR VLASGETAVV VTDVDWPAFA ESYTAARPRP LLDRIVTTAP
     SERAGEPETE SLRDRLAGLP RAERTAELVR LVRTSTATVL GHDDPKAVRA TTPFKELGFD
     SLAAVRLRNL LNAATGLRLP STLVFDHPNA SAVAGFLDAE LGTEVRGEAP SALAGLDALE
     AALPEVPATE REELVQRLER MLAALRPVAQ AADASGTGAN PSGDDLGEAG VDELLEALGR
     ELDGD
//

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