(data stored in ACNUC13479 zone)

SWISSPROT: XTH21_ARATH

ID   XTH21_ARATH             Reviewed;         305 AA.
AC   Q9ZV40;
DT   28-NOV-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1999, sequence version 1.
DT   05-JUL-2017, entry version 127.
DE   RecName: Full=Probable xyloglucan endotransglucosylase/hydrolase protein 21;
DE            Short=At-XTH21;
DE            Short=XTH-21;
DE            EC=2.4.1.207;
DE   Flags: Precursor;
GN   Name=XTH21; Synonyms=XTR17; OrderedLocusNames=At2g18800;
GN   ORFNames=MSF3.18;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; eudicotyledons; Gunneridae;
OC   Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae;
OC   Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=10617197; DOI=10.1038/45471;
RA   Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA   Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA   Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L.,
RA   Moffat K.S., Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L.,
RA   Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H.,
RA   Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D.,
RA   Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M.,
RA   Venter J.C.;
RT   "Sequence and analysis of chromosome 2 of the plant Arabidopsis
RT   thaliana.";
RL   Nature 402:761-768(1999).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RG   The Arabidopsis Information Portal (Araport);
RL   Submitted (MAY-2016) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   TISSUE SPECIFICITY, AND INDUCTION.
RX   PubMed=11673616; DOI=10.1093/pcp/pce154;
RA   Yokoyama R., Nishitani K.;
RT   "A comprehensive expression analysis of all members of a gene family
RT   encoding cell-wall enzymes allowed us to predict cis-regulatory
RT   regions involved in cell-wall construction in specific organs of
RT   Arabidopsis.";
RL   Plant Cell Physiol. 42:1025-1033(2001).
RN   [4]
RP   NOMENCLATURE.
RX   PubMed=12514239; DOI=10.1093/pcp/pcf171;
RA   Rose J.K.C., Braam J., Fry S.C., Nishitani K.;
RT   "The XTH family of enzymes involved in xyloglucan
RT   endotransglucosylation and endohydrolysis: current perspectives and a
RT   new unifying nomenclature.";
RL   Plant Cell Physiol. 43:1421-1435(2002).
CC   -!- FUNCTION: Catalyzes xyloglucan endohydrolysis (XEH) and/or
CC       endotransglycosylation (XET). Cleaves and religates xyloglucan
CC       polymers, an essential constituent of the primary cell wall, and
CC       thereby participates in cell wall construction of growing tissues
CC       (By similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY: Breaks a beta-(1->4) bond in the backbone of a
CC       xyloglucan and transfers the xyloglucanyl segment on to O-4 of the
CC       non-reducing terminal glucose residue of an acceptor, which can be
CC       a xyloglucan or an oligosaccharide of xyloglucan.
CC   -!- SUBCELLULAR LOCATION: Secreted, cell wall {ECO:0000305}. Secreted,
CC       extracellular space, apoplast {ECO:0000305}.
CC   -!- TISSUE SPECIFICITY: Predominantly expressed in green siliques.
CC       {ECO:0000269|PubMed:11673616}.
CC   -!- INDUCTION: Down-regulated by auxin. {ECO:0000269|PubMed:11673616}.
CC   -!- PTM: Contains at least one intrachain disulfide bond essential for
CC       its enzymatic activity. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 16 family. XTH group
CC       2 subfamily. {ECO:0000305}.
DR   EMBL; AC005724; AAD08949.1; -; Genomic_DNA.
DR   EMBL; CP002685; AEC06809.1; -; Genomic_DNA.
DR   PIR; G84568; G84568.
DR   RefSeq; NP_179470.1; NM_127436.2.
DR   ProteinModelPortal; Q9ZV40; -.
DR   SMR; Q9ZV40; -.
DR   STRING; 3702.AT2G18800.1; -.
DR   CAZy; GH16; Glycoside Hydrolase Family 16.
DR   PaxDb; Q9ZV40; -.
DR   EnsemblPlants; AT2G18800.1; AT2G18800.1; AT2G18800.
DR   GeneID; 816395; -.
DR   Gramene; AT2G18800.1; AT2G18800.1; AT2G18800.
DR   KEGG; ath:AT2G18800; -.
DR   Araport; AT2G18800; -.
DR   TAIR; locus:2053967; AT2G18800.
DR   eggNOG; ENOG410IHDJ; Eukaryota.
DR   eggNOG; COG2273; LUCA.
DR   HOGENOM; HOG000236368; -.
DR   InParanoid; Q9ZV40; -.
DR   KO; K08235; -.
DR   OMA; FMNYNSE; -.
DR   OrthoDB; EOG09360JBH; -.
DR   PhylomeDB; Q9ZV40; -.
DR   BioCyc; ARA:AT2G18800-MONOMER; -.
DR   BRENDA; 2.4.1.207; 399.
DR   PRO; PR:Q9ZV40; -.
DR   Proteomes; UP000006548; Chromosome 2.
DR   ExpressionAtlas; Q9ZV40; baseline and differential.
DR   Genevisible; Q9ZV40; AT.
DR   GO; GO:0048046; C:apoplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0005618; C:cell wall; IEA:UniProtKB-SubCell.
DR   GO; GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IEA:InterPro.
DR   GO; GO:0016762; F:xyloglucan:xyloglucosyl transferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0042546; P:cell wall biogenesis; IEA:InterPro.
DR   GO; GO:0042545; P:cell wall modification; IMP:TAIR.
DR   GO; GO:0080022; P:primary root development; IMP:TAIR.
DR   GO; GO:0010411; P:xyloglucan metabolic process; IEA:InterPro.
DR   InterPro; IPR013320; ConA-like_dom.
DR   InterPro; IPR000757; GH16.
DR   InterPro; IPR008263; GH16_AS.
DR   InterPro; IPR010713; XET_C.
DR   InterPro; IPR016455; XTH.
DR   Pfam; PF00722; Glyco_hydro_16; 1.
DR   Pfam; PF06955; XET_C; 1.
DR   PIRSF; PIRSF005604; XET; 1.
DR   SUPFAM; SSF49899; SSF49899; 1.
DR   PROSITE; PS01034; GH16_1; 1.
DR   PROSITE; PS51762; GH16_2; 1.
PE   2: Evidence at transcript level;
DR   PRODOM; Q9ZV40.
DR   SWISS-2DPAGE; Q9ZV40.
KW   Apoplast; Cell wall; Cell wall biogenesis/degradation;
KW   Complete proteome; Disulfide bond; Glycoprotein; Glycosidase;
KW   Hydrolase; Reference proteome; Secreted; Signal; Transferase.
FT   SIGNAL        1     25       {ECO:0000255}.
FT   CHAIN        26    305       Probable xyloglucan
FT                                endotransglucosylase/hydrolase protein
FT                                21.
FT                                /FTId=PRO_0000011821.
FT   DOMAIN       26    216       GH16. {ECO:0000255|PROSITE-
FT                                ProRule:PRU01098}.
FT   ACT_SITE    102    102       Nucleophile. {ECO:0000255|PROSITE-
FT                                ProRule:PRU10064}.
FT   ACT_SITE    106    106       Proton donor. {ECO:0000255|PROSITE-
FT                                ProRule:PRU10064}.
FT   CARBOHYD     46     46       N-linked (GlcNAc...) asparagine.
FT                                {ECO:0000255}.
FT   CARBOHYD    110    110       N-linked (GlcNAc...) asparagine.
FT                                {ECO:0000255}.
FT   CARBOHYD    146    146       N-linked (GlcNAc...) asparagine.
FT                                {ECO:0000255}.
FT   CARBOHYD    206    206       N-linked (GlcNAc...) asparagine.
FT                                {ECO:0000255}.
FT   CARBOHYD    231    231       N-linked (GlcNAc...) asparagine.
FT                                {ECO:0000255}.
SQ   SEQUENCE   305 AA;  34145 MW;  9B4BD59F25FA6DC7 CRC64;
     MVSSTLLVMS ISLFLGLSIL LVVHGKDFNQ DIDITWGDGR GNILNNGTLL NLGLDQSSGS
     GFQSKAEYLY GKVDMQIKLV PGNSAGTVTT FYLKSQGLTW DEIDFEFLGN VSGDPYIVHT
     NVYTQGKGDR EQQFYLWFDP TAAFHNYSIL WNPSHIVFYI DGKPIREFKN LEVLGVAYPK
     NQPMRMYGSL WNADDWATRG GLVKTNWSQG PFVASFMNYN SENACVWSIV NGTTTTSPCS
     PGDSTSSSSS STSEWFSQRG MDSSSKKVLR WVQRKFMVYN YCKDKKRFSN GLPVECTAKN
     KNTKS
//

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