(data stored in SCRATCH9089 zone)

SWISSPROT: RSUA_PASMU

ID   RSUA_PASMU              Reviewed;         232 AA.
AC   Q9CPN4;
DT   15-AUG-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2001, sequence version 1.
DT   11-DEC-2019, entry version 102.
DE   RecName: Full=Ribosomal small subunit pseudouridine synthase A;
DE            EC=5.4.99.19;
DE   AltName: Full=16S pseudouridylate 516 synthase;
DE   AltName: Full=16S rRNA pseudouridine(516) synthase;
DE   AltName: Full=rRNA pseudouridylate synthase A;
DE   AltName: Full=rRNA-uridine isomerase A;
GN   Name=rsuA; OrderedLocusNames=PM0003;
OS   Pasteurella multocida (strain Pm70).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales;
OC   Pasteurellaceae; Pasteurella.
OX   NCBI_TaxID=272843;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Pm70;
RX   PubMed=11248100; DOI=10.1073/pnas.051634598;
RA   May B.J., Zhang Q., Li L.L., Paustian M.L., Whittam T.S., Kapur V.;
RT   "Complete genomic sequence of Pasteurella multocida Pm70.";
RL   Proc. Natl. Acad. Sci. U.S.A. 98:3460-3465(2001).
CC   -!- FUNCTION: Responsible for synthesis of pseudouridine from uracil-516 in
CC       16S ribosomal RNA. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=uridine(516) in 16S rRNA = pseudouridine(516) in 16S rRNA;
CC         Xref=Rhea:RHEA:38867, Rhea:RHEA-COMP:10089, Rhea:RHEA-COMP:10090,
CC         ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; EC=5.4.99.19;
CC   -!- SIMILARITY: Belongs to the pseudouridine synthase RsuA family.
CC       {ECO:0000305}.
DR   EMBL; AE004439; AAK02087.1; -; Genomic_DNA.
DR   RefSeq; WP_005722236.1; NC_002663.1.
DR   SMR; Q9CPN4; -.
DR   PRIDE; Q9CPN4; -.
DR   EnsemblBacteria; AAK02087; AAK02087; PM0003.
DR   KEGG; pmu:PM0003; -.
DR   PATRIC; fig|272843.6.peg.3; -.
DR   eggNOG; ENOG4105I08; Bacteria.
DR   eggNOG; COG1187; LUCA.
DR   HOGENOM; HOG000044954; -.
DR   KO; K06183; -.
DR   OMA; HVPKTYE; -.
DR   BioCyc; PMUL272843:G1FZ8-5-MONOMER; -.
DR   Proteomes; UP000000809; Chromosome.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0120159; F:rRNA pseudouridine synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0001522; P:pseudouridine synthesis; IEA:InterPro.
DR   GO; GO:0006364; P:rRNA processing; IEA:UniProtKB-KW.
DR   CDD; cd00165; S4; 1.
DR   Gene3D; 3.10.290.10; -; 1.
DR   Gene3D; 3.30.70.1560; -; 1.
DR   InterPro; IPR042092; PsdUridine_s_RsuA/RluB/E/F_cat.
DR   InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR   InterPro; IPR006145; PsdUridine_synth_RsuA/RluA.
DR   InterPro; IPR000748; PsdUridine_synth_RsuA/RluB/E/F.
DR   InterPro; IPR018496; PsdUridine_synth_RsuA/RluB_CS.
DR   InterPro; IPR002942; S4_RNA-bd.
DR   InterPro; IPR036986; S4_RNA-bd_sf.
DR   Pfam; PF00849; PseudoU_synth_2; 1.
DR   Pfam; PF01479; S4; 1.
DR   SMART; SM00363; S4; 1.
DR   SUPFAM; SSF55120; SSF55120; 1.
DR   TIGRFAMs; TIGR00093; TIGR00093; 1.
DR   PROSITE; PS01149; PSI_RSU; 1.
DR   PROSITE; PS50889; S4; 1.
PE   3: Inferred from homology;
DR   PRODOM; Q9CPN4.
DR   SWISS-2DPAGE; Q9CPN4.
KW   Isomerase; Reference proteome; RNA-binding; rRNA processing.
FT   CHAIN           1..232
FT                   /note="Ribosomal small subunit pseudouridine synthase A"
FT                   /id="PRO_0000099970"
FT   DOMAIN          1..68
FT                   /note="S4 RNA-binding"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00182"
FT   ACT_SITE        102
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   232 AA;  26096 MW;  7B016CF8724249A7 CRC64;
     MRLDKFLAEN TGLTRSQANK VLKQSAVTVN GHVEKNGAQK VSQTDEICLE GEHLPWVSAG
     QYLMLYKPQG YVCSHEDGDY PTIYQFFDYP LAGKLHSAGR LDVDTTGLVL LTDDGKWSHR
     ITSPKHHCEK TYLVTLADPV ESHYQQACAE GMLLRGEKTP TKPATLEILD DYNVNLTISE
     GRYHQVKRMF AALGNKVVGL HRWKIGQIEL DDRLAEGEYR SLSAEEIATF NK
//

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