(data stored in SCRATCH9089 zone)

SWISSPROT: QUEC_PASMU

ID   QUEC_PASMU              Reviewed;         228 AA.
AC   Q9CP69;
DT   25-JUL-2006, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2001, sequence version 1.
DT   11-DEC-2019, entry version 96.
DE   RecName: Full=7-cyano-7-deazaguanine synthase {ECO:0000255|HAMAP-Rule:MF_01633};
DE            EC=6.3.4.20 {ECO:0000255|HAMAP-Rule:MF_01633};
DE   AltName: Full=7-cyano-7-carbaguanine synthase {ECO:0000255|HAMAP-Rule:MF_01633};
DE   AltName: Full=PreQ(0) synthase {ECO:0000255|HAMAP-Rule:MF_01633};
DE   AltName: Full=Queuosine biosynthesis protein QueC {ECO:0000255|HAMAP-Rule:MF_01633};
GN   Name=queC {ECO:0000255|HAMAP-Rule:MF_01633}; OrderedLocusNames=PM0186;
OS   Pasteurella multocida (strain Pm70).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales;
OC   Pasteurellaceae; Pasteurella.
OX   NCBI_TaxID=272843;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Pm70;
RX   PubMed=11248100; DOI=10.1073/pnas.051634598;
RA   May B.J., Zhang Q., Li L.L., Paustian M.L., Whittam T.S., Kapur V.;
RT   "Complete genomic sequence of Pasteurella multocida Pm70.";
RL   Proc. Natl. Acad. Sci. U.S.A. 98:3460-3465(2001).
CC   -!- FUNCTION: Catalyzes the ATP-dependent conversion of 7-carboxy-7-
CC       deazaguanine (CDG) to 7-cyano-7-deazaguanine (preQ(0)).
CC       {ECO:0000255|HAMAP-Rule:MF_01633}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=7-carboxy-7-deazaguanine + ATP + NH4(+) = 7-cyano-7-
CC         deazaguanine + ADP + H(+) + H2O + phosphate; Xref=Rhea:RHEA:27982,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:28938,
CC         ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:45075,
CC         ChEBI:CHEBI:61036, ChEBI:CHEBI:456216; EC=6.3.4.20;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01633};
CC   -!- COFACTOR:
CC       Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01633};
CC       Note=Binds 1 zinc ion per subunit. {ECO:0000255|HAMAP-Rule:MF_01633};
CC   -!- PATHWAY: Purine metabolism; 7-cyano-7-deazaguanine biosynthesis.
CC       {ECO:0000255|HAMAP-Rule:MF_01633}.
CC   -!- SIMILARITY: Belongs to the QueC family. {ECO:0000255|HAMAP-
CC       Rule:MF_01633}.
DR   EMBL; AE004439; AAK02270.1; -; Genomic_DNA.
DR   RefSeq; WP_010906517.1; NC_002663.1.
DR   SMR; Q9CP69; -.
DR   PRIDE; Q9CP69; -.
DR   EnsemblBacteria; AAK02270; AAK02270; PM0186.
DR   KEGG; pmu:PM0186; -.
DR   PATRIC; fig|272843.6.peg.191; -.
DR   eggNOG; ENOG4105FD2; Bacteria.
DR   eggNOG; COG0603; LUCA.
DR   HOGENOM; HOG000110563; -.
DR   KO; K06920; -.
DR   OMA; VWVPARN; -.
DR   BioCyc; PMUL272843:G1FZ8-194-MONOMER; -.
DR   UniPathway; UPA00391; -.
DR   Proteomes; UP000000809; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0016879; F:ligase activity, forming carbon-nitrogen bonds; IEA:UniProtKB-UniRule.
DR   GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0008616; P:queuosine biosynthetic process; IEA:UniProtKB-UniRule.
DR   CDD; cd01995; ExsB; 1.
DR   Gene3D; 3.40.50.620; -; 1.
DR   HAMAP; MF_01633; QueC; 1.
DR   InterPro; IPR018317; QueC.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   PANTHER; PTHR42914; PTHR42914; 1.
DR   Pfam; PF06508; QueC; 1.
DR   PIRSF; PIRSF006293; ExsB; 1.
DR   TIGRFAMs; TIGR00364; TIGR00364; 1.
PE   3: Inferred from homology;
DR   PRODOM; Q9CP69.
DR   SWISS-2DPAGE; Q9CP69.
KW   ATP-binding; Ligase; Metal-binding; Nucleotide-binding;
KW   Queuosine biosynthesis; Reference proteome; Zinc.
FT   CHAIN           1..228
FT                   /note="7-cyano-7-deazaguanine synthase"
FT                   /id="PRO_0000246872"
FT   NP_BIND         16..26
FT                   /note="ATP"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01633"
FT   METAL           193
FT                   /note="Zinc"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01633"
FT   METAL           201
FT                   /note="Zinc"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01633"
FT   METAL           204
FT                   /note="Zinc"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01633"
FT   METAL           207
FT                   /note="Zinc"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01633"
SQ   SEQUENCE   228 AA;  25665 MW;  8719AF9D08048203 CRC64;
     MQLTNPNRDR KAVVIFSGGQ DSTTCLFQAI QDYGVENVEV VTFQYGQRHA IELEKAKWIA
     QDLGVKQTLI DTSVIKAITQ NALMDEQARI EQHGSTPNTF VDGRNALFLL YTAIYAKGQG
     IQDIITGVCE TDFSGYPDCR DVFIKSMNVT LNLAMDYQFH LKTPLMYLTK AQTWQLADEL
     GVLDYIRQHT HTCYMGVEQG CGECPSCVLR EKGLREYLSS KSEAEIDV
//

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