(data stored in SCRATCH9089 zone)

SWISSPROT: WECA_PASMU

ID   WECA_PASMU              Reviewed;         357 AA.
AC   Q9CNG8;
DT   05-MAR-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2001, sequence version 1.
DT   11-DEC-2019, entry version 112.
DE   RecName: Full=Undecaprenyl-phosphate alpha-N-acetylglucosaminyl 1-phosphate transferase {ECO:0000255|HAMAP-Rule:MF_02030};
DE            EC=2.7.8.33 {ECO:0000255|HAMAP-Rule:MF_02030};
DE   AltName: Full=UDP-GlcNAc:undecaprenyl-phosphate GlcNAc-1-phosphate transferase {ECO:0000255|HAMAP-Rule:MF_02030};
DE   AltName: Full=Undecaprenyl-phosphate GlcNAc-1-phosphate transferase {ECO:0000255|HAMAP-Rule:MF_02030};
GN   Name=wecA {ECO:0000255|HAMAP-Rule:MF_02030}; Synonyms=rfe;
GN   OrderedLocusNames=PM0463;
OS   Pasteurella multocida (strain Pm70).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales;
OC   Pasteurellaceae; Pasteurella.
OX   NCBI_TaxID=272843;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Pm70;
RX   PubMed=11248100; DOI=10.1073/pnas.051634598;
RA   May B.J., Zhang Q., Li L.L., Paustian M.L., Whittam T.S., Kapur V.;
RT   "Complete genomic sequence of Pasteurella multocida Pm70.";
RL   Proc. Natl. Acad. Sci. U.S.A. 98:3460-3465(2001).
CC   -!- FUNCTION: Catalyzes the transfer of the GlcNAc-1-phosphate moiety from
CC       UDP-GlcNAc onto the carrier lipid undecaprenyl phosphate (C55-P),
CC       yielding GlcNAc-pyrophosphoryl-undecaprenyl (GlcNAc-PP-C55).
CC       {ECO:0000255|HAMAP-Rule:MF_02030}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=di-trans,octa-cis-undecaprenyl phosphate + UDP-N-acetyl-alpha-
CC         D-glucosamine = N-acetyl-alpha-D-glucosaminyl-1-diphospho-di-
CC         trans,octa-cis-undecaprenol + UMP; Xref=Rhea:RHEA:28090,
CC         ChEBI:CHEBI:57705, ChEBI:CHEBI:57865, ChEBI:CHEBI:60392,
CC         ChEBI:CHEBI:62959; EC=2.7.8.33; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_02030};
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_02030};
CC   -!- COFACTOR:
CC       Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_02030};
CC   -!- PATHWAY: Bacterial outer membrane biogenesis; LPS O-antigen
CC       biosynthesis. {ECO:0000255|HAMAP-Rule:MF_02030}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC       Rule:MF_02030}; Multi-pass membrane protein {ECO:0000255|HAMAP-
CC       Rule:MF_02030}.
CC   -!- SIMILARITY: Belongs to the glycosyltransferase 4 family. WecA
CC       subfamily. {ECO:0000255|HAMAP-Rule:MF_02030}.
DR   EMBL; AE004439; AAK02547.1; -; Genomic_DNA.
DR   RefSeq; WP_005751351.1; NC_002663.1.
DR   PRIDE; Q9CNG8; -.
DR   EnsemblBacteria; AAK02547; AAK02547; PM0463.
DR   GeneID; 29388511; -.
DR   KEGG; pmu:PM0463; -.
DR   eggNOG; ENOG4105ESV; Bacteria.
DR   eggNOG; COG0472; LUCA.
DR   HOGENOM; HOG000275125; -.
DR   KO; K02851; -.
DR   OMA; MPDNLHL; -.
DR   BioCyc; PMUL272843:G1FZ8-484-MONOMER; -.
DR   UniPathway; UPA00281; -.
DR   Proteomes; UP000000809; Chromosome.
DR   GO; GO:0009276; C:Gram-negative-bacterium-type cell wall; ISS:UniProtKB.
DR   GO; GO:0005887; C:integral component of plasma membrane; IEA:UniProtKB-UniRule.
DR   GO; GO:0000287; F:magnesium ion binding; ISS:UniProtKB.
DR   GO; GO:0030145; F:manganese ion binding; ISS:UniProtKB.
DR   GO; GO:0008963; F:phospho-N-acetylmuramoyl-pentapeptide-transferase activity; IEA:InterPro.
DR   GO; GO:0016780; F:phosphotransferase activity, for other substituted phosphate groups; ISS:UniProtKB.
DR   GO; GO:0016757; F:transferase activity, transferring glycosyl groups; IEA:UniProtKB-KW.
DR   GO; GO:0036380; F:UDP-N-acetylglucosamine-undecaprenyl-phosphate N-acetylglucosaminephosphotransferase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0044038; P:cell wall macromolecule biosynthetic process; ISS:UniProtKB.
DR   GO; GO:0071555; P:cell wall organization; ISS:UniProtKB.
DR   GO; GO:0009103; P:lipopolysaccharide biosynthetic process; ISS:UniProtKB.
DR   GO; GO:0009243; P:O antigen biosynthetic process; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_02030; WecA_Gammaproteo; 1.
DR   InterPro; IPR012750; ECA_WecA-rel.
DR   InterPro; IPR000715; Glycosyl_transferase_4.
DR   InterPro; IPR018480; PNAcMuramoyl-5peptid_Trfase_CS.
DR   PANTHER; PTHR22926; PTHR22926; 1.
DR   Pfam; PF00953; Glycos_transf_4; 1.
DR   TIGRFAMs; TIGR02380; ECA_wecA; 1.
PE   3: Inferred from homology;
DR   PRODOM; Q9CNG8.
DR   SWISS-2DPAGE; Q9CNG8.
KW   Cell inner membrane; Cell membrane; Glycosyltransferase;
KW   Lipopolysaccharide biosynthesis; Magnesium; Manganese; Membrane;
KW   Reference proteome; Transferase; Transmembrane; Transmembrane helix.
FT   CHAIN           1..357
FT                   /note="Undecaprenyl-phosphate alpha-N-acetylglucosaminyl 1-
FT                   phosphate transferase"
FT                   /id="PRO_0000108949"
FT   TRANSMEM        40..60
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_02030"
FT   TRANSMEM        64..84
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_02030"
FT   TRANSMEM        124..144
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_02030"
FT   TRANSMEM        183..203
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_02030"
FT   TRANSMEM        209..229
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_02030"
FT   TRANSMEM        238..258
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_02030"
FT   TRANSMEM        291..311
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_02030"
SQ   SEQUENCE   357 AA;  40286 MW;  B82A186EA21EED5C CRC64;
     MLLSLIVTFL GAFLTLLFMR PIAQKIGLVD KPNFRKRHQG AIPLIGGVSL FVGNLCFYLL
     EWEQMRLPTL YLFSIFVLLV IGMIDDRFDI SPFLRAGIQA ILAILMIDLG NLYLDHLGQI
     LGPFQLTLGS IGLIITVLAT IAAINAFNMI DGIDGLLGGL SSVAFVSIGI LLIKDNQLDL
     AYWSFALIIA ILPYFMMNLG IPFGQKFKVF MGDAGSTLIG FTIIWILLLS TQGKGHPMNP
     VTALWIIAIP LIDMIAIMYR RLRKGKSPFR PDRLHVHHLM MRAGLTSRQA FLLITFAAAI
     CATIGILGEI FYINEWLMFA GFILLFFMYS YSITRAWRIT RWIRRMRRRA KRIHNKG
//

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