(data stored in SCRATCH9089 zone)

SWISSPROT: SYN_PASMU

ID   SYN_PASMU               Reviewed;         467 AA.
AC   Q9CN06;
DT   31-JAN-2002, integrated into UniProtKB/Swiss-Prot.
DT   31-JAN-2002, sequence version 2.
DT   11-DEC-2019, entry version 116.
DE   RecName: Full=Asparagine--tRNA ligase {ECO:0000255|HAMAP-Rule:MF_00534};
DE            EC=6.1.1.22 {ECO:0000255|HAMAP-Rule:MF_00534};
DE   AltName: Full=Asparaginyl-tRNA synthetase {ECO:0000255|HAMAP-Rule:MF_00534};
DE            Short=AsnRS {ECO:0000255|HAMAP-Rule:MF_00534};
GN   Name=asnS {ECO:0000255|HAMAP-Rule:MF_00534}; OrderedLocusNames=PM0643;
OS   Pasteurella multocida (strain Pm70).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales;
OC   Pasteurellaceae; Pasteurella.
OX   NCBI_TaxID=272843;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Pm70;
RX   PubMed=11248100; DOI=10.1073/pnas.051634598;
RA   May B.J., Zhang Q., Li L.L., Paustian M.L., Whittam T.S., Kapur V.;
RT   "Complete genomic sequence of Pasteurella multocida Pm70.";
RL   Proc. Natl. Acad. Sci. U.S.A. 98:3460-3465(2001).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-asparagine + tRNA(Asn) = AMP + diphosphate + H(+) + L-
CC         asparaginyl-tRNA(Asn); Xref=Rhea:RHEA:11180, Rhea:RHEA-COMP:9659,
CC         Rhea:RHEA-COMP:9674, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:33019, ChEBI:CHEBI:58048, ChEBI:CHEBI:78442,
CC         ChEBI:CHEBI:78515, ChEBI:CHEBI:456215; EC=6.1.1.22;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00534};
CC   -!- SUBUNIT: Homodimer. {ECO:0000255|HAMAP-Rule:MF_00534}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00534}.
CC   -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00534}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAK02727.1; Type=Erroneous initiation; Evidence={ECO:0000305};
DR   EMBL; AE004439; AAK02727.1; ALT_INIT; Genomic_DNA.
DR   RefSeq; WP_005726506.1; NC_002663.1.
DR   SMR; Q9CN06; -.
DR   PRIDE; Q9CN06; -.
DR   EnsemblBacteria; AAK02727; AAK02727; PM0643.
DR   GeneID; 29389526; -.
DR   KEGG; pmu:PM0643; -.
DR   eggNOG; ENOG4105C5S; Bacteria.
DR   eggNOG; COG0017; LUCA.
DR   HOGENOM; HOG000226033; -.
DR   KO; K01893; -.
DR   OMA; TVRAMDI; -.
DR   BioCyc; PMUL272843:G1FZ8-676-MONOMER; -.
DR   Proteomes; UP000000809; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004816; F:asparagine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR   GO; GO:0006421; P:asparaginyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00534; Asn_tRNA_synth; 1.
DR   InterPro; IPR004364; Aa-tRNA-synt_II.
DR   InterPro; IPR006195; aa-tRNA-synth_II.
DR   InterPro; IPR004522; Asn-tRNA-ligase.
DR   InterPro; IPR002312; Asp/Asn-tRNA-synth_IIb.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   InterPro; IPR004365; NA-bd_OB_tRNA.
DR   Pfam; PF00152; tRNA-synt_2; 1.
DR   Pfam; PF01336; tRNA_anti-codon; 1.
DR   PRINTS; PR01042; TRNASYNTHASP.
DR   SUPFAM; SSF50249; SSF50249; 1.
DR   TIGRFAMs; TIGR00457; asnS; 1.
DR   PROSITE; PS50862; AA_TRNA_LIGASE_II; 1.
PE   3: Inferred from homology;
DR   PRODOM; Q9CN06.
DR   SWISS-2DPAGE; Q9CN06.
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..467
FT                   /note="Asparagine--tRNA ligase"
FT                   /id="PRO_0000176438"
SQ   SEQUENCE   467 AA;  52856 MW;  91EDD9ABCFF30E23 CRC64;
     MTKVASIVDV LQGKIAIGET VTVRGWIRTR RDSKAGLSFL AIYDGSCFDP IQAIVNNDIE
     NYETEVLRLT TGCSVIVTGT VVESPAEGQA VELQAEKVEV AGWVEDPETY PMAAKRHSIE
     YLREVAHLRP RTNIIGAVAR VRHCLAQAIH RFFHEQGFYW VATPLITASD TEGAGEMFRV
     STLDLENLPR DDKGAVDFSQ DFFGKESFLT VSGQLNGETY ACALSKIYTF GPTFRAENSN
     TTRHLAEFWM VEPEIAFATL ADNAKLAEDM LKYVFRAVLE ERKDDLKFFE KHVDNDVISR
     LENFINSDFA QIDYTDAIEV LLQSGKKFEF PVSWGIDLSS EHERYLAEEH FKSPVVVKNY
     PKDIKAFYMR LNDDGKTVAA MDVLAPGIGE IIGGSQREER LDVLDKRMIE MGLNPEDYWW
     YRDLRRYGTV PHAGFGLGFE RLIVYVTGVQ NIRDVIPFPR SPRNANF
//

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