(data stored in ACNUC7421 zone)

SWISSPROT: DBP4_ASHGO

ID   DBP4_ASHGO              Reviewed;         763 AA.
AC   Q75C76;
DT   21-MAR-2006, integrated into UniProtKB/Swiss-Prot.
DT   09-JAN-2013, sequence version 2.
DT   11-DEC-2019, entry version 103.
DE   RecName: Full=ATP-dependent RNA helicase DBP4;
DE            EC=3.6.4.13;
GN   Name=DBP4; OrderedLocusNames=ACR040W;
OS   Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056)
OS   (Yeast) (Eremothecium gossypii).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Eremothecium.
OX   NCBI_TaxID=284811;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056;
RX   PubMed=15001715; DOI=10.1126/science.1095781;
RA   Dietrich F.S., Voegeli S., Brachat S., Lerch A., Gates K., Steiner S.,
RA   Mohr C., Poehlmann R., Luedi P., Choi S., Wing R.A., Flavier A.,
RA   Gaffney T.D., Philippsen P.;
RT   "The Ashbya gossypii genome as a tool for mapping the ancient Saccharomyces
RT   cerevisiae genome.";
RL   Science 304:304-307(2004).
RN   [2]
RP   GENOME REANNOTATION, AND SEQUENCE REVISION TO 400-401.
RC   STRAIN=ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056;
RX   PubMed=23749448; DOI=10.1534/g3.112.002881;
RA   Dietrich F.S., Voegeli S., Kuo S., Philippsen P.;
RT   "Genomes of Ashbya fungi isolated from insects reveal four mating-type
RT   loci, numerous translocations, lack of transposons, and distinct gene
RT   duplications.";
RL   G3 (Bethesda) 3:1225-1239(2013).
CC   -!- FUNCTION: ATP-dependent RNA helicase required for ribosome biogenesis.
CC       Involved in the release of U14 snoRNA in pre-ribosomal complexes.
CC       Required for pre-rRNA cleavage at site A2 (By similarity).
CC       {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC   -!- SUBUNIT: Interacts with the U3 and U14 snoRNAs. Associates with pre-
CC       ribosomal complexes (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus, nucleolus {ECO:0000250}.
CC   -!- DOMAIN: The Q motif is unique to and characteristic of the DEAD box
CC       family of RNA helicases and controls ATP binding and hydrolysis.
CC   -!- SIMILARITY: Belongs to the DEAD box helicase family. DDX10/DBP4
CC       subfamily. {ECO:0000305}.
DR   EMBL; AE016816; AAS51267.2; -; Genomic_DNA.
DR   RefSeq; NP_983443.2; NM_208796.2.
DR   SMR; Q75C76; -.
DR   STRING; 33169.AAS51267; -.
DR   PRIDE; Q75C76; -.
DR   EnsemblFungi; AAS51267; AAS51267; AGOS_ACR040W.
DR   GeneID; 4619568; -.
DR   KEGG; ago:AGOS_ACR040W; -.
DR   HOGENOM; HOG000268801; -.
DR   InParanoid; Q75C76; -.
DR   KO; K14776; -.
DR   OMA; KVRTKYD; -.
DR   Proteomes; UP000000591; Chromosome III.
DR   GO; GO:0005730; C:nucleolus; IEA:UniProtKB-SubCell.
DR   GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR   GO; GO:0032040; C:small-subunit processome; IEA:EnsemblFungi.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0034512; F:box C/D snoRNA binding; IEA:EnsemblFungi.
DR   GO; GO:0042802; F:identical protein binding; IEA:EnsemblFungi.
DR   GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR   GO; GO:0003724; F:RNA helicase activity; IEA:UniProtKB-EC.
DR   GO; GO:0008186; F:RNA-dependent ATPase activity; IEA:EnsemblFungi.
DR   GO; GO:0034511; F:U3 snoRNA binding; IEA:EnsemblFungi.
DR   GO; GO:0006364; P:rRNA processing; IBA:GO_Central.
DR   InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR   InterPro; IPR025313; DUF4217.
DR   InterPro; IPR014001; Helicase_ATP-bd.
DR   InterPro; IPR001650; Helicase_C.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR000629; RNA-helicase_DEAD-box_CS.
DR   InterPro; IPR014014; RNA_helicase_DEAD_Q_motif.
DR   Pfam; PF00270; DEAD; 1.
DR   Pfam; PF13959; DUF4217; 1.
DR   Pfam; PF00271; Helicase_C; 1.
DR   SMART; SM00487; DEXDc; 1.
DR   SMART; SM01178; DUF4217; 1.
DR   SMART; SM00490; HELICc; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS00039; DEAD_ATP_HELICASE; 1.
DR   PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR   PROSITE; PS51194; HELICASE_CTER; 1.
DR   PROSITE; PS51195; Q_MOTIF; 1.
PE   3: Inferred from homology;
DR   PRODOM; Q75C76.
DR   SWISS-2DPAGE; Q75C76.
KW   ATP-binding; Helicase; Hydrolase; Nucleotide-binding; Nucleus;
KW   Reference proteome; Ribosome biogenesis; RNA-binding; rRNA processing.
FT   CHAIN           1..763
FT                   /note="ATP-dependent RNA helicase DBP4"
FT                   /id="PRO_0000227946"
FT   DOMAIN          72..246
FT                   /note="Helicase ATP-binding"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT   DOMAIN          276..435
FT                   /note="Helicase C-terminal"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00542"
FT   NP_BIND         85..92
FT                   /note="ATP"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT   MOTIF           41..69
FT                   /note="Q motif"
FT   MOTIF           194..197
FT                   /note="DEAD box"
SQ   SEQUENCE   763 AA;  85655 MW;  1908A958BE3812D8 CRC64;
     MAKKHRLTTV KRKELRQKES EALKQLQERI KDYDAKTCRA VTFQDLPISS GTVKGLKEAA
     YIKMTDIQRA AIPVALKGHD VLGAAKTGSG KTLAFLVPVL EKLYHERWTE LDGLGALVIS
     PTRELAMQIY EVLVKIGKYM SFSAGLVIGG KDVKFEMERV SKINILIGTP GRLLQHMDQS
     VGLNTSNLQI LVLDEADRCL DMGFKKALDA IVSNLPPSRQ TLLFSATQSQ SLADLARLSL
     ADYKTVGTMD GPSSKNKPAT PENLEQFYIQ VALPDKLDIL FSFIKSHLKS KMIVFLSSSK
     QVHFVYETFR KMQPGISLMH LHGRQKQTAR TETLDKFSRA QHVCLFSTDV VARGIDFPSV
     DWVIQTDCPE DVDTYIHRAG RSARYGKTGK SLLMLTPQEE DAFLARLKGK LIEPSKLNIK
     QSKRKSIKPQ LQSLLFKDPE LKYLAQKAFI SYVRSIYIQK DTEVFKFNEL PLEEFAASLG
     LPGAPQVKIK GKKSIEKAKE LKNTSRQLLL LAKANDDGEV DSKEKTVRTK YDKMFGRKNQ
     TVLSEHYMNI TKTGAADDDA GDFMTIKRTD HELNENDLPQ LTLPTSKRGQ KKALSKKASL
     SSKGNPTKFK FDADGVPHPI YELEGEEEFH KKGDVDTQVK EFLSKETEYM ARADTEDKQL
     VKEKRQEKKR KRLEAMRREM EALQDDDDEY DAPTVAYVGT GNLSDDMASG DEPDFAPASP
     PPRKKLKGRY SESAESPDTG VMEVEEPDTL EDLESLTARL ISG
//

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