(data stored in SCRATCH3701 zone)

SWISSPROT: SAM_RICTY

ID   SAM_RICTY               Reviewed;         294 AA.
AC   Q68XV0;
DT   20-MAR-2007, integrated into UniProtKB/Swiss-Prot.
DT   11-OCT-2004, sequence version 1.
DT   11-DEC-2019, entry version 74.
DE   RecName: Full=S-adenosylmethionine uptake transporter;
GN   Name=sam; OrderedLocusNames=RT0056;
OS   Rickettsia typhi (strain ATCC VR-144 / Wilmington).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rickettsiales;
OC   Rickettsiaceae; Rickettsieae; Rickettsia; typhus group.
OX   NCBI_TaxID=257363;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC VR-144 / Wilmington;
RX   PubMed=15317790; DOI=10.1128/jb.186.17.5842-5855.2004;
RA   McLeod M.P., Qin X., Karpathy S.E., Gioia J., Highlander S.K., Fox G.E.,
RA   McNeill T.Z., Jiang H., Muzny D., Jacob L.S., Hawes A.C., Sodergren E.,
RA   Gill R., Hume J., Morgan M., Fan G., Amin A.G., Gibbs R.A., Hong C.,
RA   Yu X.-J., Walker D.H., Weinstock G.M.;
RT   "Complete genome sequence of Rickettsia typhi and comparison with sequences
RT   of other Rickettsiae.";
RL   J. Bacteriol. 186:5842-5855(2004).
RN   [2]
RP   FUNCTION, AND ACTIVITY REGULATION.
RC   STRAIN=ATCC VR-144 / Wilmington;
RX   PubMed=12730161; DOI=10.1128/jb.185.10.3031-3035.2003;
RA   Tucker A.M., Winkler H.H., Driskell L.O., Wood D.O.;
RT   "S-adenosylmethionine transport in Rickettsia prowazekii.";
RL   J. Bacteriol. 185:3031-3035(2003).
CC   -!- FUNCTION: Transports S-adenosylmethionine.
CC       {ECO:0000269|PubMed:12730161}.
CC   -!- ACTIVITY REGULATION: Transport is inhibited by S-adenosylethionine and
CC       to a lesser extent by S-adenosylhomocysteine. Unlike eukaryotic
CC       transporters is not inhibited by sinfungin. Also inhibited by 2.4-
CC       dinitrophenol, suggesting transport is an energy-dependent process.
CC       {ECO:0000269|PubMed:12730161}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000305}; Multi-pass
CC       membrane protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the drug/metabolite transporter (DMT)
CC       superfamily. 10 TMS drug/metabolite exporter (DME) (TC 2.A.7.3) family.
CC       {ECO:0000305}.
DR   EMBL; AE017197; AAU03542.1; -; Genomic_DNA.
DR   RefSeq; WP_011190529.1; NC_006142.1.
DR   STRING; 257363.RT0056; -.
DR   EnsemblBacteria; AAU03542; AAU03542; RT0056.
DR   KEGG; rty:RT0056; -.
DR   eggNOG; ENOG4105DFI; Bacteria.
DR   eggNOG; COG0697; LUCA.
DR   HOGENOM; HOG000264283; -.
DR   KO; K15270; -.
DR   OMA; DWGWMAL; -.
DR   OrthoDB; 1420919at2; -.
DR   BioCyc; RTYP257363:G1G0L-57-MONOMER; -.
DR   Proteomes; UP000000604; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0006865; P:amino acid transport; IEA:UniProtKB-KW.
DR   InterPro; IPR000620; EamA_dom.
DR   Pfam; PF00892; EamA; 2.
PE   3: Inferred from homology;
DR   PRODOM; Q68XV0.
DR   SWISS-2DPAGE; Q68XV0.
KW   Amino-acid transport; Cell inner membrane; Cell membrane; Membrane; Repeat;
KW   Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..294
FT                   /note="S-adenosylmethionine uptake transporter"
FT                   /id="PRO_0000280998"
FT   TRANSMEM        8..28
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        41..61
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        72..91
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        98..118
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        121..141
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        148..168
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        178..198
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        207..227
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        237..257
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        260..280
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          23..141
FT                   /note="EamA 1"
FT   DOMAIN          160..280
FT                   /note="EamA 2"
SQ   SEQUENCE   294 AA;  33141 MW;  34778945A87EF02A CRC64;
     MNDALKTYLK GICWFLLSLV TSSVNDVISK YLGTRLHSFE VAFFRFLFSS IVLLPFVVYY
     GKSTLKTRHP VIHVLRGLLL FFGMTSWTYG LTIAPVTTAT VISFSIPLFT LILAVFILNE
     NIIWQRWVVT VVGFIGLVVM LKPHTKDFNP EILYLILAAI SFAMLDIINK KFVVKESMLS
     MLFYSAIVTA IVSLPVSMEY WITPSSFELT LLFVLGSSGS FILFFLLKAF TMVDATATAP
     YRYLELVISA IAAYFIFNEF PDKSTAHGAV IIIPATLFIM YSEKKSMSSK HESQ
//

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