(data stored in SCRATCH3701 zone)

SWISSPROT: Y532_RICTY

ID   Y532_RICTY              Reviewed;         242 AA.
AC   Q68WJ1;
DT   10-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT   11-OCT-2004, sequence version 1.
DT   11-DEC-2019, entry version 80.
DE   RecName: Full=Uncharacterized RNA pseudouridine synthase RT0532;
DE            EC=5.4.99.-;
DE   AltName: Full=RNA pseudouridylate synthase;
DE   AltName: Full=RNA-uridine isomerase;
GN   OrderedLocusNames=RT0532;
OS   Rickettsia typhi (strain ATCC VR-144 / Wilmington).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rickettsiales;
OC   Rickettsiaceae; Rickettsieae; Rickettsia; typhus group.
OX   NCBI_TaxID=257363;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC VR-144 / Wilmington;
RX   PubMed=15317790; DOI=10.1128/jb.186.17.5842-5855.2004;
RA   McLeod M.P., Qin X., Karpathy S.E., Gioia J., Highlander S.K., Fox G.E.,
RA   McNeill T.Z., Jiang H., Muzny D., Jacob L.S., Hawes A.C., Sodergren E.,
RA   Gill R., Hume J., Morgan M., Fan G., Amin A.G., Gibbs R.A., Hong C.,
RA   Yu X.-J., Walker D.H., Weinstock G.M.;
RT   "Complete genome sequence of Rickettsia typhi and comparison with sequences
RT   of other Rickettsiae.";
RL   J. Bacteriol. 186:5842-5855(2004).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a uridine in RNA = a pseudouridine in RNA;
CC         Xref=Rhea:RHEA:48348, Rhea:RHEA-COMP:12068, Rhea:RHEA-COMP:12069,
CC         ChEBI:CHEBI:65314, ChEBI:CHEBI:65315;
CC   -!- SIMILARITY: Belongs to the pseudouridine synthase RsuA family.
CC       {ECO:0000305}.
DR   EMBL; AE017197; AAU04001.1; -; Genomic_DNA.
DR   RefSeq; WP_011190982.1; NC_006142.1.
DR   SMR; Q68WJ1; -.
DR   STRING; 257363.RT0532; -.
DR   EnsemblBacteria; AAU04001; AAU04001; RT0532.
DR   KEGG; rty:RT0532; -.
DR   eggNOG; ENOG4105FE9; Bacteria.
DR   eggNOG; COG1187; LUCA.
DR   HOGENOM; HOG000044954; -.
DR   KO; K06178; -.
DR   OMA; SMEFAPF; -.
DR   OrthoDB; 1037615at2; -.
DR   BioCyc; RTYP257363:G1G0L-538-MONOMER; -.
DR   Proteomes; UP000000604; Chromosome.
DR   GO; GO:0009982; F:pseudouridine synthase activity; IEA:InterPro.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0001522; P:pseudouridine synthesis; IEA:InterPro.
DR   CDD; cd00165; S4; 1.
DR   Gene3D; 3.10.290.10; -; 1.
DR   Gene3D; 3.30.70.1560; -; 1.
DR   InterPro; IPR042092; PsdUridine_s_RsuA/RluB/E/F_cat.
DR   InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR   InterPro; IPR006145; PsdUridine_synth_RsuA/RluA.
DR   InterPro; IPR000748; PsdUridine_synth_RsuA/RluB/E/F.
DR   InterPro; IPR018496; PsdUridine_synth_RsuA/RluB_CS.
DR   InterPro; IPR002942; S4_RNA-bd.
DR   InterPro; IPR036986; S4_RNA-bd_sf.
DR   Pfam; PF00849; PseudoU_synth_2; 1.
DR   Pfam; PF01479; S4; 1.
DR   SMART; SM00363; S4; 1.
DR   SUPFAM; SSF55120; SSF55120; 1.
DR   TIGRFAMs; TIGR00093; TIGR00093; 1.
DR   PROSITE; PS01149; PSI_RSU; 1.
DR   PROSITE; PS50889; S4; 1.
PE   3: Inferred from homology;
DR   PRODOM; Q68WJ1.
DR   SWISS-2DPAGE; Q68WJ1.
KW   Isomerase; RNA-binding.
FT   CHAIN           1..242
FT                   /note="Uncharacterized RNA pseudouridine synthase RT0532"
FT                   /id="PRO_0000294477"
FT   DOMAIN          2..69
FT                   /note="S4 RNA-binding"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00182"
FT   ACT_SITE        102
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   242 AA;  27756 MW;  A422912F78AD4188 CRC64;
     MYRLAKIISN AGVCSRRNAE KLIVGGKVKI DGITILSPAT NVDISNQIEV SGRLINNIQK
     PRLWIYYKPI GLITTHKDPL SRKTVFEQLI GLPRVISIGR LDLNSEGLLL LTNSGDLAHQ
     FEMPSSKLKR VYNVRAYGNA NFLLKNNYNN LKIDGIFYNP YSIKLLRQNK NNSWFEVVLF
     EGKNREIRRI FEYFGLKVNK LIRVQYGALK IGNLKPGDYK EISNKILKKI ISNKLTNYID
     NR
//

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