(data stored in SCRATCH zone)

SWISSPROT: SYGB_PSEU2

ID   SYGB_PSEU2              Reviewed;         684 AA.
AC   Q500T7;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   07-JUN-2005, sequence version 1.
DT   11-DEC-2019, entry version 95.
DE   RecName: Full=Glycine--tRNA ligase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            EC=6.1.1.14 {ECO:0000255|HAMAP-Rule:MF_00255};
DE   AltName: Full=Glycyl-tRNA synthetase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            Short=GlyRS {ECO:0000255|HAMAP-Rule:MF_00255};
GN   Name=glyS {ECO:0000255|HAMAP-Rule:MF_00255}; OrderedLocusNames=Psyr_0011;
OS   Pseudomonas syringae pv. syringae (strain B728a).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas; Pseudomonas syringae.
OX   NCBI_TaxID=205918;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=B728a;
RX   PubMed=16043691; DOI=10.1073/pnas.0504930102;
RA   Feil H., Feil W.S., Chain P., Larimer F., Dibartolo G., Copeland A.,
RA   Lykidis A., Trong S., Nolan M., Goltsman E., Thiel J., Malfatti S.,
RA   Loper J.E., Lapidus A., Detter J.C., Land M., Richardson P.M.,
RA   Kyrpides N.C., Ivanova N., Lindow S.E.;
RT   "Comparison of the complete genome sequences of Pseudomonas syringae pv.
RT   syringae B728a and pv. tomato DC3000.";
RL   Proc. Natl. Acad. Sci. U.S.A. 102:11064-11069(2005).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-
CC         tRNA(Gly); Xref=Rhea:RHEA:16013, Rhea:RHEA-COMP:9664, Rhea:RHEA-
CC         COMP:9683, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57305,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78522, ChEBI:CHEBI:456215;
CC         EC=6.1.1.14; Evidence={ECO:0000255|HAMAP-Rule:MF_00255};
CC   -!- SUBUNIT: Tetramer of two alpha and two beta subunits.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
DR   EMBL; CP000075; AAY35085.1; -; Genomic_DNA.
DR   RefSeq; WP_003401442.1; NC_007005.1.
DR   RefSeq; YP_233123.1; NC_007005.1.
DR   SMR; Q500T7; -.
DR   STRING; 205918.Psyr_0011; -.
DR   PRIDE; Q500T7; -.
DR   EnsemblBacteria; AAY35085; AAY35085; Psyr_0011.
DR   GeneID; 3365486; -.
DR   KEGG; psb:Psyr_0011; -.
DR   PATRIC; fig|205918.7.peg.11; -.
DR   eggNOG; ENOG4105C38; Bacteria.
DR   eggNOG; COG0751; LUCA.
DR   HOGENOM; HOG000264302; -.
DR   KO; K01879; -.
DR   OMA; LPIPKRM; -.
DR   BioCyc; PSYR205918:G1G4J-11-MONOMER; -.
DR   Proteomes; UP000000426; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004814; F:arginine-tRNA ligase activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004820; F:glycine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006420; P:arginyl-tRNA aminoacylation; IEA:InterPro.
DR   GO; GO:0006426; P:glycyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00255; Gly_tRNA_synth_beta; 1.
DR   InterPro; IPR008909; DALR_anticod-bd.
DR   InterPro; IPR015944; Gly-tRNA-synth_bsu.
DR   InterPro; IPR006194; Gly-tRNA-synth_heterodimer.
DR   PANTHER; PTHR30075; PTHR30075; 1.
DR   Pfam; PF05746; DALR_1; 1.
DR   Pfam; PF02092; tRNA_synt_2f; 1.
DR   PRINTS; PR01045; TRNASYNTHGB.
DR   TIGRFAMs; TIGR00211; glyS; 1.
DR   PROSITE; PS50861; AA_TRNA_LIGASE_II_GLYAB; 1.
PE   3: Inferred from homology;
DR   PRODOM; Q500T7.
DR   SWISS-2DPAGE; Q500T7.
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis.
FT   CHAIN           1..684
FT                   /note="Glycine--tRNA ligase beta subunit"
FT                   /id="PRO_1000006394"
SQ   SEQUENCE   684 AA;  74849 MW;  C7C56758646538B2 CRC64;
     MSAQDFLVEL GTEELPPKTL VSLADAFLAG IEKGLAGAGL TYSAKQVYAA PRRLAVLITA
     LATQQPDRSV NLDGPPRQAA FDADGNPTQA ALGFAKKCGV DLSEIDQSGP KLRYSQTILG
     KPTTSLLPTI VEDSLNDLPI AKRMRWGARK EEFVRPTQWL VMLFGDQVVD CTILAQSAGR
     HSRGHRFHHP QDVRISSPAG YLSDLRAAHV LADFNERRQI ISKRVDELAT QQEGTAIVPP
     SLLDEVAGLV EWPVPLVCSF EERFLEVPQE ALITTMQDNQ KYFCLLDVDG KLLPRFITVA
     NIESKDPAQI IAGNEKVVRP RLTDAEFFFK QDKKQKLETF NDRLKNVVFQ AQLGSVFDKA
     ERVSKLAAYI APRIGGDAQR AARAGLLSKC DLSSEMVGEF PEMQGIAGYY YAKADGEPED
     VALALNEQYM PRGAGAELPT TLTGAAVAIA DKLDTLVGIF GIGMLPTGSK DPYALRRAAL
     GILRILIEKK LDLNLVETVK FAVAQFGAKI KPAGLAEQVL DFIFDRLRAR YEDEGVDVAV
     YLSVRALQPA SALDFDQRVQ AVQAFRKLPQ AAALAAVNKR VSNLLSKAEG SIAQTVEPKY
     FDNANEFSLY SAIQQADHAI QPMAAERQYS ESLARLAMLR EPVDAFFEAV MVNAEDANVR
     ANRYALLSRL RGLFLGVADI SLLG
//

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