(data stored in ACNUC7421 zone)

SWISSPROT: PCKA_PSEU2

ID   PCKA_PSEU2              Reviewed;         514 AA.
AC   Q500D1;
DT   30-MAY-2006, integrated into UniProtKB/Swiss-Prot.
DT   07-JUN-2005, sequence version 1.
DT   08-MAY-2019, entry version 81.
DE   RecName: Full=Phosphoenolpyruvate carboxykinase (ATP) {ECO:0000255|HAMAP-Rule:MF_00453};
DE            Short=PCK {ECO:0000255|HAMAP-Rule:MF_00453};
DE            Short=PEP carboxykinase {ECO:0000255|HAMAP-Rule:MF_00453};
DE            Short=PEPCK {ECO:0000255|HAMAP-Rule:MF_00453};
DE            EC=4.1.1.49 {ECO:0000255|HAMAP-Rule:MF_00453};
GN   Name=pckA {ECO:0000255|HAMAP-Rule:MF_00453};
GN   OrderedLocusNames=Psyr_0168;
OS   Pseudomonas syringae pv. syringae (strain B728a).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas; Pseudomonas syringae.
OX   NCBI_TaxID=205918;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=B728a;
RX   PubMed=16043691; DOI=10.1073/pnas.0504930102;
RA   Feil H., Feil W.S., Chain P., Larimer F., Dibartolo G., Copeland A.,
RA   Lykidis A., Trong S., Nolan M., Goltsman E., Thiel J., Malfatti S.,
RA   Loper J.E., Lapidus A., Detter J.C., Land M., Richardson P.M.,
RA   Kyrpides N.C., Ivanova N., Lindow S.E.;
RT   "Comparison of the complete genome sequences of Pseudomonas syringae
RT   pv. syringae B728a and pv. tomato DC3000.";
RL   Proc. Natl. Acad. Sci. U.S.A. 102:11064-11069(2005).
CC   -!- FUNCTION: Involved in the gluconeogenesis. Catalyzes the
CC       conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP)
CC       through direct phosphoryl transfer between the nucleoside
CC       triphosphate and OAA. {ECO:0000255|HAMAP-Rule:MF_00453}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + oxaloacetate = ADP + CO2 + phosphoenolpyruvate;
CC         Xref=Rhea:RHEA:18617, ChEBI:CHEBI:16452, ChEBI:CHEBI:16526,
CC         ChEBI:CHEBI:30616, ChEBI:CHEBI:58702, ChEBI:CHEBI:456216;
CC         EC=4.1.1.49; Evidence={ECO:0000255|HAMAP-Rule:MF_00453};
CC   -!- COFACTOR:
CC       Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00453};
CC       Note=Binds 1 Mn(2+) ion per subunit. {ECO:0000255|HAMAP-
CC       Rule:MF_00453};
CC   -!- PATHWAY: Carbohydrate biosynthesis; gluconeogenesis.
CC       {ECO:0000255|HAMAP-Rule:MF_00453}.
CC   -!- SUBUNIT: Monomer. {ECO:0000255|HAMAP-Rule:MF_00453}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00453}.
CC   -!- SIMILARITY: Belongs to the phosphoenolpyruvate carboxykinase (ATP)
CC       family. {ECO:0000255|HAMAP-Rule:MF_00453}.
DR   EMBL; CP000075; AAY35241.1; -; Genomic_DNA.
DR   RefSeq; WP_003367573.1; NC_007005.1.
DR   RefSeq; YP_233279.1; NC_007005.1.
DR   SMR; Q500D1; -.
DR   STRING; 205918.Psyr_0168; -.
DR   EnsemblBacteria; AAY35241; AAY35241; Psyr_0168.
DR   GeneID; 3365644; -.
DR   KEGG; psb:Psyr_0168; -.
DR   PATRIC; fig|205918.7.peg.164; -.
DR   eggNOG; ENOG4105DJ1; Bacteria.
DR   eggNOG; COG1866; LUCA.
DR   HOGENOM; HOG000271471; -.
DR   KO; K01610; -.
DR   OMA; MRYAGEM; -.
DR   BioCyc; PSYR205918:G1G4J-169-MONOMER; -.
DR   UniPathway; UPA00138; -.
DR   Proteomes; UP000000426; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0004612; F:phosphoenolpyruvate carboxykinase (ATP) activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006094; P:gluconeogenesis; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.40.449.10; -; 1.
DR   Gene3D; 3.90.228.20; -; 1.
DR   HAMAP; MF_00453; PEPCK_ATP; 1.
DR   InterPro; IPR001272; PEP_carboxykinase_ATP.
DR   InterPro; IPR013035; PEP_carboxykinase_C.
DR   InterPro; IPR008210; PEP_carboxykinase_N.
DR   InterPro; IPR015994; PEPCK_ATP_CS.
DR   PANTHER; PTHR30031; PTHR30031; 1.
DR   Pfam; PF01293; PEPCK_ATP; 1.
DR   PIRSF; PIRSF006294; PEP_crbxkin; 1.
DR   SUPFAM; SSF68923; SSF68923; 1.
DR   TIGRFAMs; TIGR00224; pckA; 1.
DR   PROSITE; PS00532; PEPCK_ATP; 1.
PE   3: Inferred from homology;
DR   PRODOM; Q500D1.
DR   SWISS-2DPAGE; Q500D1.
KW   ATP-binding; Complete proteome; Cytoplasm; Decarboxylase;
KW   Gluconeogenesis; Lyase; Manganese; Metal-binding; Nucleotide-binding.
FT   CHAIN         1    514       Phosphoenolpyruvate carboxykinase (ATP).
FT                                /FTId=PRO_0000236936.
FT   NP_BIND     220    228       ATP. {ECO:0000255|HAMAP-Rule:MF_00453}.
FT   METAL       185    185       Manganese. {ECO:0000255|HAMAP-
FT                                Rule:MF_00453}.
FT   METAL       204    204       Manganese; via tele nitrogen.
FT                                {ECO:0000255|HAMAP-Rule:MF_00453}.
FT   METAL       241    241       Manganese. {ECO:0000255|HAMAP-
FT                                Rule:MF_00453}.
FT   BINDING      45     45       Substrate. {ECO:0000255|HAMAP-
FT                                Rule:MF_00453}.
FT   BINDING     179    179       Substrate. {ECO:0000255|HAMAP-
FT                                Rule:MF_00453}.
FT   BINDING     185    185       ATP. {ECO:0000255|HAMAP-Rule:MF_00453}.
FT   BINDING     185    185       Substrate. {ECO:0000255|HAMAP-
FT                                Rule:MF_00453}.
FT   BINDING     204    204       ATP. {ECO:0000255|HAMAP-Rule:MF_00453}.
FT   BINDING     269    269       ATP. {ECO:0000255|HAMAP-Rule:MF_00453}.
FT   BINDING     306    306       ATP. {ECO:0000255|HAMAP-Rule:MF_00453}.
FT   BINDING     306    306       Substrate. {ECO:0000255|HAMAP-
FT                                Rule:MF_00453}.
FT   BINDING     432    432       ATP. {ECO:0000255|HAMAP-Rule:MF_00453}.
SQ   SEQUENCE   514 AA;  55856 MW;  508CAA9F7FCDA3E7 CRC64;
     MTQTNNAVYT DLSTDELVKE ALARKEGVLS DTGALVVETG HRTGRSPVDR FIVEEPSTQD
     AIAWGPINRK FPAEKFDALW NRVGAYLAER ERFVSHVHVG SDPAHYLPVK MTTETAWHNL
     FGRCLFINPE QYNPAGKDEW EIQNAPWFVC DPERDGTNSD GTVIINFAAR KVLIAGMRYA
     GEMKKAMFSV QNFLLPASDV LPMHCAANMG EEGDVTLFFG LSGTGKTTLS ADESRYLIGD
     DEHGWGEGVV FNIEGGCYAK CIDLSEKNEP VIWKAIQHGA VLENVVLDPV TKKADYADSS
     LTQNSRAAYP RELIEKRAPK NLGGEPNAVI FLTCDLTGVL PPVSILSEEQ AAYHFLSGYT
     ALVGSTEMGS GSGIKSTFST CFGAPFFPRP AGEYAELLIK RIRGFKSKVY LVNTGWTGGG
     YGVGKRFNIP TTRGVIAAIQ SGALIGAETE HLDTINLDVP KSVPGVDTGL LNPRNTWADK
     AAYDEAAKAL AGLFVENFKK FDVSDAIKAA GPKL
//

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