(data stored in ACNUC7421 zone)

SWISSPROT: POTA_THEFY

ID   POTA_THEFY              Reviewed;         384 AA.
AC   Q47T99;
DT   01-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT   13-SEP-2005, sequence version 1.
DT   11-DEC-2019, entry version 106.
DE   RecName: Full=Spermidine/putrescine import ATP-binding protein PotA {ECO:0000255|HAMAP-Rule:MF_01726};
DE            EC=7.6.2.11 {ECO:0000255|HAMAP-Rule:MF_01726};
GN   Name=potA {ECO:0000255|HAMAP-Rule:MF_01726}; OrderedLocusNames=Tfu_0280;
OS   Thermobifida fusca (strain YX).
OC   Bacteria; Actinobacteria; Streptosporangiales; Nocardiopsaceae;
OC   Thermobifida.
OX   NCBI_TaxID=269800;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=YX;
RX   PubMed=17209016; DOI=10.1128/jb.01899-06;
RA   Lykidis A., Mavromatis K., Ivanova N., Anderson I., Land M., DiBartolo G.,
RA   Martinez M., Lapidus A., Lucas S., Copeland A., Richardson P., Wilson D.B.,
RA   Kyrpides N.;
RT   "Genome sequence and analysis of the soil cellulolytic actinomycete
RT   Thermobifida fusca YX.";
RL   J. Bacteriol. 189:2477-2486(2007).
CC   -!- FUNCTION: Part of the ABC transporter complex PotABCD involved in
CC       spermidine/putrescine import. Responsible for energy coupling to the
CC       transport system. {ECO:0000255|HAMAP-Rule:MF_01726}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H(2)O + polyamine-[polyamine-binding protein](Side 1) =
CC         ADP + phosphate + polyamine(Side 2) + [polyamine-binding
CC         protein](Side 1).; EC=7.6.2.11; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_01726};
CC   -!- SUBUNIT: The complex is composed of two ATP-binding proteins (PotA),
CC       two transmembrane proteins (PotB and PotC) and a solute-binding protein
CC       (PotD). {ECO:0000255|HAMAP-Rule:MF_01726}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000255|HAMAP-Rule:MF_01726};
CC       Peripheral membrane protein {ECO:0000255|HAMAP-Rule:MF_01726}.
CC   -!- SIMILARITY: Belongs to the ABC transporter superfamily.
CC       Spermidine/putrescine importer (TC 3.A.1.11.1) family.
CC       {ECO:0000255|HAMAP-Rule:MF_01726}.
DR   EMBL; CP000088; AAZ54318.1; -; Genomic_DNA.
DR   RefSeq; WP_011290727.1; NC_007333.1.
DR   SMR; Q47T99; -.
DR   STRING; 269800.Tfu_0280; -.
DR   EnsemblBacteria; AAZ54318; AAZ54318; Tfu_0280.
DR   KEGG; tfu:Tfu_0280; -.
DR   eggNOG; ENOG4105C53; Bacteria.
DR   eggNOG; COG3842; LUCA.
DR   KO; K11072; -.
DR   OMA; RLHSGQM; -.
DR   OrthoDB; 1200451at2; -.
DR   BioCyc; TFUS269800:G1G4Q-283-MONOMER; -.
DR   Proteomes; UP000000434; Chromosome.
DR   GO; GO:0043190; C:ATP-binding cassette (ABC) transporter complex; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0016887; F:ATPase activity; IEA:InterPro.
DR   GO; GO:0015594; F:ATPase-coupled putrescine transmembrane transporter activity; IEA:InterPro.
DR   GO; GO:0015595; F:ATPase-coupled spermidine transmembrane transporter activity; IEA:InterPro.
DR   InterPro; IPR003593; AAA+_ATPase.
DR   InterPro; IPR017879; ABC_Sperm/Put_ATP-bd_PotA.
DR   InterPro; IPR003439; ABC_transporter-like.
DR   InterPro; IPR017871; ABC_transporter_CS.
DR   InterPro; IPR008995; Mo/tungstate-bd_C_term_dom.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR005893; Sp_pt_ABC_ATP-bd.
DR   InterPro; IPR013611; Transp-assoc_OB_typ2.
DR   Pfam; PF00005; ABC_tran; 1.
DR   Pfam; PF08402; TOBE_2; 1.
DR   SMART; SM00382; AAA; 1.
DR   SUPFAM; SSF50331; SSF50331; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR01187; potA; 1.
DR   PROSITE; PS00211; ABC_TRANSPORTER_1; 1.
DR   PROSITE; PS50893; ABC_TRANSPORTER_2; 1.
DR   PROSITE; PS51305; POTA; 1.
PE   3: Inferred from homology;
DR   PRODOM; Q47T99.
DR   SWISS-2DPAGE; Q47T99.
KW   ATP-binding; Cell membrane; Membrane; Nucleotide-binding; Translocase;
KW   Transport.
FT   CHAIN           1..384
FT                   /note="Spermidine/putrescine import ATP-binding protein
FT                   PotA"
FT                   /id="PRO_0000286318"
FT   DOMAIN          26..260
FT                   /note="ABC transporter"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01726"
FT   NP_BIND         62..69
FT                   /note="ATP"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01726"
SQ   SEQUENCE   384 AA;  42033 MW;  738CB7C9266E31E6 CRC64;
     MATTTEKTPA APGVVATESN QAVPAISLEK VSKTYRSGQV TTTAVESIDL EIRQGEFFSL
     LGPSGCGKTT TMRMIAGFEE PTSGVVRLSG KDVTGVPPNR RDVNMVFQSY ALFPHMTVAE
     NVAFGLKRKK VPAAEIRTRV AEMLELVELG DRAKYKPRQL SGGQQQRVAL ARALVNRPSA
     LLLDEPLGAL DLKLRQAMQL ELKRIQREVG ITFVYVTHDQ SEALTMSDRI AVMNKGRIEQ
     LGTPAQIYET PATRFVAGFI GTSNILTGTA QRVSDTLVRI DYGGDQHVLA NTPQQMSDLG
     LVITVRPEKI RLGKDVPDAN VSRIRGTVRE VVYLGATTHY TVRTVDDTDI VVFQQNSSDA
     SNLADHGDTV WLSWRPEHSY VLPG
//

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