(data stored in ACNUC7421 zone)

SWISSPROT: Q3KJW5_PSEPF

ID   Q3KJW5_PSEPF            Unreviewed;       329 AA.
AC   Q3KJW5;
DT   08-NOV-2005, integrated into UniProtKB/TrEMBL.
DT   08-NOV-2005, sequence version 1.
DT   08-MAY-2019, entry version 129.
DE   RecName: Full=Sulfate/thiosulfate import ATP-binding protein CysA {ECO:0000256|HAMAP-Rule:MF_01701};
DE            EC=7.3.2.3 {ECO:0000256|HAMAP-Rule:MF_01701};
DE   AltName: Full=Sulfate-transporting ATPase {ECO:0000256|HAMAP-Rule:MF_01701};
GN   Name=cysA {ECO:0000256|HAMAP-Rule:MF_01701};
GN   OrderedLocusNames=Pfl01_0197 {ECO:0000313|EMBL:ABA71941.1};
OS   Pseudomonas fluorescens (strain Pf0-1).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=205922 {ECO:0000313|EMBL:ABA71941.1, ECO:0000313|Proteomes:UP000002704};
RN   [1] {ECO:0000313|EMBL:ABA71941.1, ECO:0000313|Proteomes:UP000002704}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Pf0-1 {ECO:0000313|EMBL:ABA71941.1,
RC   ECO:0000313|Proteomes:UP000002704};
RX   PubMed=19432983; DOI=10.1186/gb-2009-10-5-r51;
RA   Silby M.W., Cerdeno-Tarraga A.M., Vernikos G.S., Giddens S.R.,
RA   Jackson R.W., Preston G.M., Zhang X.X., Moon C.D., Gehrig S.M.,
RA   Godfrey S.A., Knight C.G., Malone J.G., Robinson Z., Spiers A.J.,
RA   Harris S., Challis G.L., Yaxley A.M., Harris D., Seeger K., Murphy L.,
RA   Rutter S., Squares R., Quail M.A., Saunders E., Mavromatis K.,
RA   Brettin T.S., Bentley S.D., Hothersall J., Stephens E., Thomas C.M.,
RA   Parkhill J., Levy S.B., Rainey P.B., Thomson N.R.;
RT   "Genomic and genetic analyses of diversity and plant interactions of
RT   Pseudomonas fluorescens.";
RL   Genome Biol. 10:R51.1-R51.16(2009).
CC   -!- FUNCTION: Part of the ABC transporter complex CysAWTP involved in
CC       sulfate/thiosulfate import. Responsible for energy coupling to the
CC       transport system. {ECO:0000256|HAMAP-Rule:MF_01701,
CC       ECO:0000256|SAAS:SAAS00360330}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O + sulfate(out) = ADP + H(+) + phosphate +
CC         sulfate(in); Xref=Rhea:RHEA:10192, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:16189, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=7.3.2.3;
CC         Evidence={ECO:0000256|HAMAP-Rule:MF_01701,
CC         ECO:0000256|SAAS:SAAS01131877};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O + thiosulfate(out) = ADP + H(+) + phosphate +
CC         thiosulfate(in); Xref=Rhea:RHEA:29871, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:33542,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=7.3.2.3;
CC         Evidence={ECO:0000256|HAMAP-Rule:MF_01701,
CC         ECO:0000256|SAAS:SAAS01131887};
CC   -!- SUBUNIT: The complex is composed of two ATP-binding proteins
CC       (CysA), two transmembrane proteins (CysT and CysW) and a solute-
CC       binding protein (CysP). {ECO:0000256|HAMAP-Rule:MF_01701,
CC       ECO:0000256|SAAS:SAAS00360324}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000256|HAMAP-
CC       Rule:MF_01701}; Peripheral membrane protein {ECO:0000256|HAMAP-
CC       Rule:MF_01701}.
CC   -!- SIMILARITY: Belongs to the ABC transporter superfamily.
CC       Sulfate/tungstate importer (TC 3.A.1.6) family.
CC       {ECO:0000256|HAMAP-Rule:MF_01701, ECO:0000256|SAAS:SAAS00552311}.
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DR   EMBL; CP000094; ABA71941.1; -; Genomic_DNA.
DR   RefSeq; WP_011331906.1; NC_007492.2.
DR   STRING; 205922.Pfl01_0197; -.
DR   PRIDE; Q3KJW5; -.
DR   EnsemblBacteria; ABA71941; ABA71941; Pfl01_0197.
DR   KEGG; pfo:Pfl01_0197; -.
DR   eggNOG; ENOG4108IJ6; Bacteria.
DR   eggNOG; COG1118; LUCA.
DR   KO; K02045; -.
DR   OMA; MEVRVQN; -.
DR   BioCyc; PFLU205922:G1G4S-200-MONOMER; -.
DR   Proteomes; UP000002704; Chromosome.
DR   GO; GO:0043190; C:ATP-binding cassette (ABC) transporter complex; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0015419; F:ATPase-coupled sulfate transmembrane transporter activity; IEA:UniProtKB-UniRule.
DR   CDD; cd03296; ABC_CysA_sulfate_importer; 1.
DR   InterPro; IPR003593; AAA+_ATPase.
DR   InterPro; IPR014769; ABC_CysA_ATP-bd_C.
DR   InterPro; IPR003439; ABC_transporter-like.
DR   InterPro; IPR017871; ABC_transporter_CS.
DR   InterPro; IPR008995; Mo/tungstate-bd_C_term_dom.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR005666; Sulph_transpt1.
DR   InterPro; IPR024765; TOBE-like.
DR   Pfam; PF00005; ABC_tran; 1.
DR   Pfam; PF12857; TOBE_3; 1.
DR   SMART; SM00382; AAA; 1.
DR   SUPFAM; SSF50331; SSF50331; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR00968; 3a0106s01; 1.
DR   PROSITE; PS00211; ABC_TRANSPORTER_1; 1.
DR   PROSITE; PS50893; ABC_TRANSPORTER_2; 1.
DR   PROSITE; PS51237; CYSA; 1.
PE   3: Inferred from homology;
DR   PRODOM; Q3KJW5.
DR   SWISS-2DPAGE; Q3KJW5.
KW   ATP-binding {ECO:0000256|HAMAP-Rule:MF_01701, ECO:0000256|PROSITE-
KW   ProRule:PRU00434, ECO:0000256|SAAS:SAAS00237035,
KW   ECO:0000313|EMBL:ABA71941.1};
KW   Cell inner membrane {ECO:0000256|HAMAP-Rule:MF_01701};
KW   Cell membrane {ECO:0000256|HAMAP-Rule:MF_01701,
KW   ECO:0000256|SAAS:SAAS00437629};
KW   Complete proteome {ECO:0000313|Proteomes:UP000002704};
KW   Hydrolase {ECO:0000313|EMBL:ABA71941.1};
KW   Membrane {ECO:0000256|HAMAP-Rule:MF_01701,
KW   ECO:0000256|SAAS:SAAS00437643};
KW   Nucleotide-binding {ECO:0000256|HAMAP-Rule:MF_01701,
KW   ECO:0000256|PROSITE-ProRule:PRU00434, ECO:0000256|SAAS:SAAS00452314};
KW   Sulfate transport {ECO:0000256|HAMAP-Rule:MF_01701,
KW   ECO:0000256|SAAS:SAAS00437635};
KW   Translocase {ECO:0000256|HAMAP-Rule:MF_01701,
KW   ECO:0000256|SAAS:SAAS01131881};
KW   Transport {ECO:0000256|HAMAP-Rule:MF_01701,
KW   ECO:0000256|SAAS:SAAS00237071}.
FT   DOMAIN        3    237       ABC transporter. {ECO:0000259|PROSITE:
FT                                PS50893}.
FT   DOMAIN      197    322       CYSA. {ECO:0000259|PROSITE:PS51237}.
FT   NP_BIND      35     42       ATP. {ECO:0000256|PROSITE-ProRule:
FT                                PRU00434}.
SQ   SEQUENCE   329 AA;  37024 MW;  1CC4875A6BE6E265 CRC64;
     MSIEVRNVSK NFNAFKALDN ISLDIHSGEL VALLGPSGCG KTTLLRIIAG LETPDNGNIV
     FHGEDVSGHD VRDRNVGFVF QHYALFRHMT VFDNVAFGLR MKPKNQRPNE SQIATKVHEL
     LNMVQLDWLS DRYPEQLSGG QRQRIALARA LAVEPKVLLL DEPFGALDAK VRKELRRWLA
     RLHEDINLTS VFVTHDQEEA MEVADRIVVM NKGVIEQIGS PGDVYENPAS DFVYHFLGDS
     NRLHLGDDNH VLFRPHEVSL SRHELEDHHA AEVRDIRPLG ATTRVTLKVE GQTDLIEAEV
     VKDHDSLTGL AKGETLFFKP KVWQKVANV
//

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