(data stored in ACNUC7421 zone)

SWISSPROT: GSH1_PSEPF

ID   GSH1_PSEPF              Reviewed;         532 AA.
AC   Q3KJQ5;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   08-NOV-2005, sequence version 1.
DT   08-MAY-2019, entry version 90.
DE   RecName: Full=Glutamate--cysteine ligase {ECO:0000255|HAMAP-Rule:MF_00578};
DE            EC=6.3.2.2 {ECO:0000255|HAMAP-Rule:MF_00578};
DE   AltName: Full=Gamma-ECS {ECO:0000255|HAMAP-Rule:MF_00578};
DE            Short=GCS {ECO:0000255|HAMAP-Rule:MF_00578};
DE   AltName: Full=Gamma-glutamylcysteine synthetase {ECO:0000255|HAMAP-Rule:MF_00578};
GN   Name=gshA {ECO:0000255|HAMAP-Rule:MF_00578};
GN   OrderedLocusNames=Pfl01_0257;
OS   Pseudomonas fluorescens (strain Pf0-1).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=205922;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Pf0-1;
RX   PubMed=19432983; DOI=10.1186/gb-2009-10-5-r51;
RA   Silby M.W., Cerdeno-Tarraga A.M., Vernikos G.S., Giddens S.R.,
RA   Jackson R.W., Preston G.M., Zhang X.-X., Moon C.D., Gehrig S.M.,
RA   Godfrey S.A.C., Knight C.G., Malone J.G., Robinson Z., Spiers A.J.,
RA   Harris S., Challis G.L., Yaxley A.M., Harris D., Seeger K., Murphy L.,
RA   Rutter S., Squares R., Quail M.A., Saunders E., Mavromatis K.,
RA   Brettin T.S., Bentley S.D., Hothersall J., Stephens E., Thomas C.M.,
RA   Parkhill J., Levy S.B., Rainey P.B., Thomson N.R.;
RT   "Genomic and genetic analyses of diversity and plant interactions of
RT   Pseudomonas fluorescens.";
RL   Genome Biol. 10:R51.1-R51.16(2009).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-cysteine + L-glutamate = ADP + H(+) + L-gamma-
CC         glutamyl-L-cysteine + phosphate; Xref=Rhea:RHEA:13285,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:29985, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:35235, ChEBI:CHEBI:43474, ChEBI:CHEBI:58173,
CC         ChEBI:CHEBI:456216; EC=6.3.2.2; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_00578};
CC   -!- PATHWAY: Sulfur metabolism; glutathione biosynthesis; glutathione
CC       from L-cysteine and L-glutamate: step 1/2. {ECO:0000255|HAMAP-
CC       Rule:MF_00578}.
CC   -!- SIMILARITY: Belongs to the glutamate--cysteine ligase type 1
CC       family. Type 1 subfamily. {ECO:0000255|HAMAP-Rule:MF_00578}.
DR   EMBL; CP000094; ABA72001.1; -; Genomic_DNA.
DR   SMR; Q3KJQ5; -.
DR   STRING; 205922.Pfl01_0257; -.
DR   PRIDE; Q3KJQ5; -.
DR   EnsemblBacteria; ABA72001; ABA72001; Pfl01_0257.
DR   KEGG; pfo:Pfl01_0257; -.
DR   eggNOG; ENOG4105D9M; Bacteria.
DR   eggNOG; COG2918; LUCA.
DR   HOGENOM; HOG000266224; -.
DR   KO; K01919; -.
DR   OMA; RYSWLLM; -.
DR   UniPathway; UPA00142; UER00209.
DR   Proteomes; UP000002704; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004357; F:glutamate-cysteine ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006750; P:glutathione biosynthetic process; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00578; Glu_cys_ligase; 1.
DR   InterPro; IPR014746; Gln_synth/guanido_kin_cat_dom.
DR   InterPro; IPR007370; Glu_cys_ligase.
DR   InterPro; IPR006334; Glut_cys_ligase.
DR   PANTHER; PTHR38761; PTHR38761; 1.
DR   Pfam; PF04262; Glu_cys_ligase; 1.
DR   SUPFAM; SSF55931; SSF55931; 1.
DR   TIGRFAMs; TIGR01434; glu_cys_ligase; 1.
PE   3: Inferred from homology;
DR   PRODOM; Q3KJQ5.
DR   SWISS-2DPAGE; Q3KJQ5.
KW   ATP-binding; Complete proteome; Glutathione biosynthesis; Ligase;
KW   Nucleotide-binding.
FT   CHAIN         1    532       Glutamate--cysteine ligase.
FT                                /FTId=PRO_1000025182.
SQ   SEQUENCE   532 AA;  59302 MW;  4A96C284BB0BC5F6 CRC64;
     MKESKLSELL NRRLALLGER ANLSLLEQCL HGIERECLRV TGEGRLAQTP HPEALGSALT
     NEQITTDYSE SLLEFITPAL PDPADTLASL DKIHRFAYSK LGNEYLWSPS MPCPLPAEED
     IPIAYYGTSN IGQLKYVYRK GLALRYGKTM QCIAGIHYNF SLPEKLWPLL KEAEGFVGTD
     RDFQSSSYIA LIRNFRRYSW LLMYLFGASP ALDAGFLRGR AHQLEQLDPD TLYLPYATSL
     RMSDLGYQSN AQAGLTPCYN DLASYTDSLR KAVATPYAPY VEVGTHQDGE WVQLNTNILQ
     IENEYYSNIR PKRVTYTGER PIQALMARGI QYVEVRCLDI NPFLPMGIDL TESRFLDAFL
     LYCALNESPL LTNNSCGNAT SNFLSVVKEG RRPGLQLQRD GQPVELKEWA AELLEKIAPL
     AALLDQSHGG DAHSKALDAQ LAKVKDSSLT PSAQVLAAMA AHKESFAQFS LRQSQAHAEF
     FRSEPLAAEE QAKFEELARS SLAQQAELEQ NEVGDFDVFV GSYQASILAI SN
//

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