(data stored in ACNUC7421 zone)

SWISSPROT: Q2IM85_ANADE

ID   Q2IM85_ANADE            Unreviewed;       418 AA.
AC   Q2IM85;
DT   07-MAR-2006, integrated into UniProtKB/TrEMBL.
DT   07-MAR-2006, sequence version 1.
DT   08-MAY-2019, entry version 98.
DE   RecName: Full=DNA polymerase IV {ECO:0000256|HAMAP-Rule:MF_01113};
DE            Short=Pol IV {ECO:0000256|HAMAP-Rule:MF_01113};
DE            EC=2.7.7.7 {ECO:0000256|HAMAP-Rule:MF_01113};
GN   Name=dinB {ECO:0000256|HAMAP-Rule:MF_01113};
GN   OrderedLocusNames=Adeh_0141 {ECO:0000313|EMBL:ABC79918.1};
OS   Anaeromyxobacter dehalogenans (strain 2CP-C).
OC   Bacteria; Proteobacteria; Deltaproteobacteria; Myxococcales;
OC   Cystobacterineae; Anaeromyxobacteraceae; Anaeromyxobacter.
OX   NCBI_TaxID=290397 {ECO:0000313|EMBL:ABC79918.1, ECO:0000313|Proteomes:UP000001935};
RN   [1] {ECO:0000313|EMBL:ABC79918.1, ECO:0000313|Proteomes:UP000001935}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=2CP-C {ECO:0000313|EMBL:ABC79918.1,
RC   ECO:0000313|Proteomes:UP000001935};
RG   US DOE Joint Genome Institute;
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T.,
RA   Hammon N., Israni S., Pitluck S., Brettin T., Bruce D., Han C.,
RA   Tapia R., Gilna P., Kiss H., Schmutz J., Larimer F., Land M.,
RA   Kyrpides N., Anderson I., Sanford R.A., Ritalahti K.M., Thomas H.S.,
RA   Kirby J.R., Zhulin I.B., Loeffler F.E., Richardson P.;
RT   "Complete sequence of Anaeromyxobacter dehalogenans 2CP-C.";
RL   Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases.
RN   [2] {ECO:0000313|Proteomes:UP000001935}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=2CP-C {ECO:0000313|Proteomes:UP000001935};
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T.,
RA   Hammon N., Israni S., Pitluck S., Brettin T., Bruce D., Han C.,
RA   Tapia R., Gilna P., Kiss H., Schmutz J., Larimer F., Land M.,
RA   Kyrpides N., Anderson I., Sanford R.A., Ritalahti K.M., Thomas H.S.,
RA   Kirby J.R., Zhulin I.B., Loeffler F.E., Richardson P.;
RT   "Complete sequence of Anaeromyxobacter dehalogenans 2CP-C.";
RL   Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Poorly processive, error-prone DNA polymerase involved
CC       in untargeted mutagenesis. Copies undamaged DNA at stalled
CC       replication forks, which arise in vivo from mismatched or
CC       misaligned primer ends. These misaligned primers can be extended
CC       by PolIV. Exhibits no 3'-5' exonuclease (proofreading) activity.
CC       May be involved in translesional synthesis, in conjunction with
CC       the beta clamp from PolIII. {ECO:0000256|HAMAP-Rule:MF_01113}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC         diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-
CC         COMP:11130, Rhea:RHEA-COMP:11131, ChEBI:CHEBI:33019,
CC         ChEBI:CHEBI:61560, ChEBI:CHEBI:83828; EC=2.7.7.7;
CC         Evidence={ECO:0000256|HAMAP-Rule:MF_01113,
CC         ECO:0000256|SAAS:SAAS01115616};
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000256|HAMAP-
CC         Rule:MF_01113};
CC       Note=Binds 2 magnesium ions per subunit. {ECO:0000256|HAMAP-
CC       Rule:MF_01113};
CC   -!- SUBUNIT: Monomer. {ECO:0000256|HAMAP-Rule:MF_01113,
CC       ECO:0000256|SAAS:SAAS00737835}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_01113,
CC       ECO:0000256|SAAS:SAAS00737775}.
CC   -!- SIMILARITY: Belongs to the DNA polymerase type-Y family.
CC       {ECO:0000256|HAMAP-Rule:MF_01113, ECO:0000256|SAAS:SAAS00538466}.
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DR   EMBL; CP000251; ABC79918.1; -; Genomic_DNA.
DR   RefSeq; WP_011419201.1; NC_007760.1.
DR   STRING; 290397.Adeh_0141; -.
DR   EnsemblBacteria; ABC79918; ABC79918; Adeh_0141.
DR   KEGG; ade:Adeh_0141; -.
DR   eggNOG; ENOG4105CQ3; Bacteria.
DR   eggNOG; COG0389; LUCA.
DR   HOGENOM; HOG000082707; -.
DR   KO; K02346; -.
DR   OMA; GVWIRIG; -.
DR   BioCyc; ADEH290397:G1G5W-145-MONOMER; -.
DR   Proteomes; UP000001935; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003684; F:damaged DNA binding; IEA:InterPro.
DR   GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule.
DR   GO; GO:0006261; P:DNA-dependent DNA replication; IEA:UniProtKB-UniRule.
DR   CDD; cd03586; PolY_Pol_IV_kappa; 1.
DR   Gene3D; 3.30.1490.100; -; 1.
DR   HAMAP; MF_01113; DNApol_IV; 1.
DR   InterPro; IPR036775; DNA_pol_Y-fam_lit_finger_sf.
DR   InterPro; IPR017961; DNA_pol_Y-fam_little_finger.
DR   InterPro; IPR022880; DNApol_IV.
DR   InterPro; IPR024728; PolY_HhH_motif.
DR   InterPro; IPR001126; UmuC.
DR   Pfam; PF00817; IMS; 1.
DR   Pfam; PF11799; IMS_C; 1.
DR   Pfam; PF11798; IMS_HHH; 1.
DR   SUPFAM; SSF100879; SSF100879; 1.
DR   PROSITE; PS50173; UMUC; 1.
PE   3: Inferred from homology;
DR   PRODOM; Q2IM85.
DR   SWISS-2DPAGE; Q2IM85.
KW   Complete proteome {ECO:0000313|Proteomes:UP000001935};
KW   Cytoplasm {ECO:0000256|HAMAP-Rule:MF_01113,
KW   ECO:0000256|SAAS:SAAS00737824};
KW   DNA damage {ECO:0000256|HAMAP-Rule:MF_01113,
KW   ECO:0000256|SAAS:SAAS00737805};
KW   DNA repair {ECO:0000256|HAMAP-Rule:MF_01113,
KW   ECO:0000256|SAAS:SAAS00737784};
KW   DNA replication {ECO:0000256|HAMAP-Rule:MF_01113,
KW   ECO:0000256|SAAS:SAAS00737790};
KW   DNA-binding {ECO:0000256|HAMAP-Rule:MF_01113,
KW   ECO:0000256|SAAS:SAAS00737844};
KW   DNA-directed DNA polymerase {ECO:0000256|HAMAP-Rule:MF_01113,
KW   ECO:0000256|SAAS:SAAS00737803, ECO:0000313|EMBL:ABC79918.1};
KW   Magnesium {ECO:0000256|HAMAP-Rule:MF_01113,
KW   ECO:0000256|SAAS:SAAS00737808};
KW   Metal-binding {ECO:0000256|HAMAP-Rule:MF_01113,
KW   ECO:0000256|SAAS:SAAS00737787};
KW   Mutator protein {ECO:0000256|HAMAP-Rule:MF_01113};
KW   Nucleotidyltransferase {ECO:0000256|HAMAP-Rule:MF_01113,
KW   ECO:0000256|SAAS:SAAS00737766, ECO:0000313|EMBL:ABC79918.1};
KW   Reference proteome {ECO:0000313|Proteomes:UP000001935};
KW   Transferase {ECO:0000256|HAMAP-Rule:MF_01113,
KW   ECO:0000256|SAAS:SAAS00737757, ECO:0000313|EMBL:ABC79918.1}.
FT   DOMAIN       13    193       UmuC. {ECO:0000259|PROSITE:PS50173}.
FT   ACT_SITE    112    112       {ECO:0000256|HAMAP-Rule:MF_01113}.
FT   METAL        17     17       Magnesium. {ECO:0000256|HAMAP-Rule:
FT                                MF_01113}.
FT   METAL       111    111       Magnesium. {ECO:0000256|HAMAP-Rule:
FT                                MF_01113}.
FT   SITE         22     22       Substrate discrimination.
FT                                {ECO:0000256|HAMAP-Rule:MF_01113}.
SQ   SEQUENCE   418 AA;  44263 MW;  EADAC343AD717BCB CRC64;
     MYRRPMTPGP RTILHLDLDA FYASVEQLDH PELRGRPVIV GGTGNRGVVC AASYEARRFG
     VRSALPTARA RRLCPDGVFL PPRFGRYSEL SRRVFDVYRR YTPLVEPLSL DEAFLDVTAS
     RALHGGGPDI ARAIKAAVRG ECGLAVSAGV ADVKMAAKIA TDLGKPDGLV VVPPGGVAAF
     LAPLPVGRLW GVGEVTEAAL RRIGVATIGD LARMPESALA AALGPTHARG LRALACGEDP
     REVVPDEEAK SVGAEDTFDQ DLLGRPALER ELLAQAGTVA RRLRAAGLAG HVVTLKVKYA
     DFTLVTRRVT LERATDDDRA IFDAACAQLA RVDLRRPVRL TGISVSGFAG DEERGQLGLF
     GGAPAPPPPE ASRRKALNAA LDALAGRFGE GAVTRADLAG RPPRRRHGDP EDGGDPEG
//

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