(data stored in SCRATCH zone)

SWISSPROT: TRUA_PARXL

ID   TRUA_PARXL              Reviewed;         270 AA.
AC   Q13S27;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   22-AUG-2006, sequence version 1.
DT   08-MAY-2019, entry version 83.
DE   RecName: Full=tRNA pseudouridine synthase A {ECO:0000255|HAMAP-Rule:MF_00171};
DE            EC=5.4.99.12 {ECO:0000255|HAMAP-Rule:MF_00171};
DE   AltName: Full=tRNA pseudouridine(38-40) synthase {ECO:0000255|HAMAP-Rule:MF_00171};
DE   AltName: Full=tRNA pseudouridylate synthase I {ECO:0000255|HAMAP-Rule:MF_00171};
DE   AltName: Full=tRNA-uridine isomerase I {ECO:0000255|HAMAP-Rule:MF_00171};
GN   Name=truA {ECO:0000255|HAMAP-Rule:MF_00171};
GN   OrderedLocusNames=Bxeno_B0144; ORFNames=Bxe_B2883;
OS   Paraburkholderia xenovorans (strain LB400).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Burkholderiaceae; Paraburkholderia.
OX   NCBI_TaxID=266265;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=LB400;
RX   PubMed=17030797; DOI=10.1073/pnas.0606924103;
RA   Chain P.S.G., Denef V.J., Konstantinidis K.T., Vergez L.M., Agullo L.,
RA   Reyes V.L., Hauser L., Cordova M., Gomez L., Gonzalez M., Land M.,
RA   Lao V., Larimer F., LiPuma J.J., Mahenthiralingam E., Malfatti S.A.,
RA   Marx C.J., Parnell J.J., Ramette A., Richardson P., Seeger M.,
RA   Smith D., Spilker T., Sul W.J., Tsoi T.V., Ulrich L.E., Zhulin I.B.,
RA   Tiedje J.M.;
RT   "Burkholderia xenovorans LB400 harbors a multi-replicon, 9.73-Mbp
RT   genome shaped for versatility.";
RL   Proc. Natl. Acad. Sci. U.S.A. 103:15280-15287(2006).
CC   -!- FUNCTION: Formation of pseudouridine at positions 38, 39 and 40 in
CC       the anticodon stem and loop of transfer RNAs. {ECO:0000255|HAMAP-
CC       Rule:MF_00171}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=tRNA uridine(38-40) = tRNA pseudouridine(38-40);
CC         Xref=Rhea:RHEA:22376, Rhea:RHEA-COMP:10085, Rhea:RHEA-
CC         COMP:10087, ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; EC=5.4.99.12;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00171};
CC   -!- SUBUNIT: Homodimer. {ECO:0000255|HAMAP-Rule:MF_00171}.
CC   -!- SIMILARITY: Belongs to the tRNA pseudouridine synthase TruA
CC       family. {ECO:0000255|HAMAP-Rule:MF_00171}.
DR   EMBL; CP000271; ABE33112.1; -; Genomic_DNA.
DR   RefSeq; WP_011490495.1; NZ_CP008762.1.
DR   STRING; 266265.Bxe_B2883; -.
DR   EnsemblBacteria; ABE33112; ABE33112; Bxe_B2883.
DR   GeneID; 4006752; -.
DR   KEGG; bxb:DR64_5213; -.
DR   KEGG; bxe:Bxe_B2883; -.
DR   PATRIC; fig|266265.5.peg.4806; -.
DR   eggNOG; ENOG4105DI7; Bacteria.
DR   eggNOG; COG0101; LUCA.
DR   HOGENOM; HOG000248672; -.
DR   KO; K06173; -.
DR   OMA; FLYGMVR; -.
DR   OrthoDB; 1075262at2; -.
DR   BioCyc; BXEN266265:BXE_RS22780-MONOMER; -.
DR   Proteomes; UP000001817; Chromosome 2.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0106029; F:tRNA pseudouridine synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0031119; P:tRNA pseudouridine synthesis; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.30.70.660; -; 1.
DR   HAMAP; MF_00171; TruA; 1.
DR   InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR   InterPro; IPR001406; PsdUridine_synth_TruA.
DR   InterPro; IPR020097; PsdUridine_synth_TruA_a/b_dom.
DR   InterPro; IPR020095; PsdUridine_synth_TruA_C.
DR   PANTHER; PTHR11142; PTHR11142; 1.
DR   Pfam; PF01416; PseudoU_synth_1; 2.
DR   PIRSF; PIRSF001430; tRNA_psdUrid_synth; 1.
DR   SUPFAM; SSF55120; SSF55120; 1.
DR   TIGRFAMs; TIGR00071; hisT_truA; 1.
PE   3: Inferred from homology;
DR   PRODOM; Q13S27.
DR   SWISS-2DPAGE; Q13S27.
KW   Complete proteome; Isomerase; Reference proteome; tRNA processing.
FT   CHAIN         1    270       tRNA pseudouridine synthase A.
FT                                /FTId=PRO_1000017061.
FT   ACT_SITE     52     52       Nucleophile. {ECO:0000255|HAMAP-
FT                                Rule:MF_00171}.
FT   BINDING     110    110       Substrate. {ECO:0000255|HAMAP-
FT                                Rule:MF_00171}.
SQ   SEQUENCE   270 AA;  30540 MW;  86DB98BAB90763DC CRC64;
     MKRIALGVQY DGSVFCGWQS QPHGNTVQDE LERALREFAQ TPVQTIVAGR TDTGVHGLGQ
     VVHFDTELDR ADVSWVRGTN SFLPKTISVQ WAKPMPDEFH ARFSAFERTY YYVLYVHPVR
     SPMLAARAGW VHAALDVDAM QAAAAHLIGE HDFSAFRSSQ CQSKTPVKHL YQIDVRQQGD
     FIHFRFRANA FLHHMVRNLM GCLVYIGRGR RPVEWMAEVL ASRDREFAAP TFMPDGLYLA
     QVGYPEQFAV PAPQTGSVPW STVWTEQAQT
//

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