(data stored in SCRATCH zone)

SWISSPROT: Q13RX8_PARXL

ID   Q13RX8_PARXL            Unreviewed;       913 AA.
AC   Q13RX8;
DT   22-AUG-2006, integrated into UniProtKB/TrEMBL.
DT   22-AUG-2006, sequence version 1.
DT   08-MAY-2019, entry version 105.
DE   RecName: Full=DNA polymerase I {ECO:0000256|RuleBase:RU004460};
DE            EC=2.7.7.7 {ECO:0000256|RuleBase:RU004460};
GN   Name=polA {ECO:0000256|RuleBase:RU004460};
GN   ORFNames=Bxe_B2834 {ECO:0000313|EMBL:ABE33161.1};
OS   Paraburkholderia xenovorans (strain LB400).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Burkholderiaceae; Paraburkholderia.
OX   NCBI_TaxID=266265 {ECO:0000313|EMBL:ABE33161.1, ECO:0000313|Proteomes:UP000001817};
RN   [1] {ECO:0000313|EMBL:ABE33161.1, ECO:0000313|Proteomes:UP000001817}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=LB400 {ECO:0000313|EMBL:ABE33161.1,
RC   ECO:0000313|Proteomes:UP000001817};
RX   PubMed=17030797; DOI=10.1073/pnas.0606924103;
RA   Chain P.S., Denef V.J., Konstantinidis K.T., Vergez L.M., Agullo L.,
RA   Reyes V.L., Hauser L., Cordova M., Gomez L., Gonzalez M., Land M.,
RA   Lao V., Larimer F., LiPuma J.J., Mahenthiralingam E., Malfatti S.A.,
RA   Marx C.J., Parnell J.J., Ramette A., Richardson P., Seeger M.,
RA   Smith D., Spilker T., Sul W.J., Tsoi T.V., Ulrich L.E., Zhulin I.B.,
RA   Tiedje J.M.;
RT   "Burkholderia xenovorans LB400 harbors a multi-replicon, 9.73-Mbp
RT   genome shaped for versatility.";
RL   Proc. Natl. Acad. Sci. U.S.A. 103:15280-15287(2006).
CC   -!- FUNCTION: In addition to polymerase activity, this DNA polymerase
CC       exhibits 5'-3' exonuclease activity.
CC       {ECO:0000256|RuleBase:RU004460}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC         diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-
CC         COMP:11130, Rhea:RHEA-COMP:11131, ChEBI:CHEBI:33019,
CC         ChEBI:CHEBI:61560, ChEBI:CHEBI:83828; EC=2.7.7.7;
CC         Evidence={ECO:0000256|RuleBase:RU004460};
CC   -!- SIMILARITY: Belongs to the DNA polymerase type-A family.
CC       {ECO:0000256|RuleBase:RU004460}.
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DR   EMBL; CP000271; ABE33161.1; -; Genomic_DNA.
DR   RefSeq; WP_011490538.1; NZ_CP008762.1.
DR   STRING; 266265.Bxe_B2834; -.
DR   EnsemblBacteria; ABE33161; ABE33161; Bxe_B2834.
DR   GeneID; 4006567; -.
DR   KEGG; bxb:DR64_5163; -.
DR   KEGG; bxe:Bxe_B2834; -.
DR   PATRIC; fig|266265.5.peg.4859; -.
DR   eggNOG; ENOG4105C2M; Bacteria.
DR   eggNOG; COG0258; LUCA.
DR   eggNOG; COG0749; LUCA.
DR   HOGENOM; HOG000020999; -.
DR   KO; K02335; -.
DR   OMA; ETGRVHT; -.
DR   OrthoDB; 1220182at2; -.
DR   BioCyc; BXEN266265:BXE_RS23030-MONOMER; -.
DR   Proteomes; UP000001817; Chromosome 2.
DR   GO; GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule.
DR   GO; GO:0006261; P:DNA-dependent DNA replication; IEA:UniProtKB-UniRule.
DR   CDD; cd09898; H3TH_53EXO; 1.
DR   Gene3D; 3.30.420.10; -; 1.
DR   InterPro; IPR002562; 3'-5'_exonuclease_dom.
DR   InterPro; IPR020046; 5-3_exonucl_a-hlix_arch_N.
DR   InterPro; IPR036279; 5-3_exonuclease_C_sf.
DR   InterPro; IPR002421; 5-3_exonuclease_N.
DR   InterPro; IPR019760; DNA-dir_DNA_pol_A_CS.
DR   InterPro; IPR001098; DNA-dir_DNA_pol_A_palm_dom.
DR   InterPro; IPR020045; DNA_polI_H3TH.
DR   InterPro; IPR018320; DNA_polymerase_1.
DR   InterPro; IPR002298; DNA_polymerase_A.
DR   InterPro; IPR008918; HhH2.
DR   InterPro; IPR029060; PIN-like_dom_sf.
DR   InterPro; IPR012337; RNaseH-like_sf.
DR   InterPro; IPR036397; RNaseH_sf.
DR   PANTHER; PTHR10133; PTHR10133; 1.
DR   Pfam; PF01367; 5_3_exonuc; 1.
DR   Pfam; PF02739; 5_3_exonuc_N; 1.
DR   Pfam; PF00476; DNA_pol_A; 1.
DR   Pfam; PF01612; DNA_pol_A_exo1; 1.
DR   PRINTS; PR00868; DNAPOLI.
DR   SMART; SM00474; 35EXOc; 1.
DR   SMART; SM00475; 53EXOc; 1.
DR   SMART; SM00279; HhH2; 1.
DR   SMART; SM00482; POLAc; 1.
DR   SUPFAM; SSF47807; SSF47807; 1.
DR   SUPFAM; SSF53098; SSF53098; 1.
DR   SUPFAM; SSF88723; SSF88723; 1.
DR   TIGRFAMs; TIGR00593; pola; 1.
DR   PROSITE; PS00447; DNA_POLYMERASE_A; 1.
PE   3: Inferred from homology;
DR   PRODOM; Q13RX8.
DR   SWISS-2DPAGE; Q13RX8.
KW   Coiled coil {ECO:0000256|SAM:Coils};
KW   Complete proteome {ECO:0000313|Proteomes:UP000001817};
KW   DNA damage {ECO:0000256|RuleBase:RU004460};
KW   DNA repair {ECO:0000256|RuleBase:RU004460};
KW   DNA replication {ECO:0000256|RuleBase:RU004460};
KW   DNA-binding {ECO:0000256|RuleBase:RU004460,
KW   ECO:0000256|SAAS:SAAS00882656};
KW   DNA-directed DNA polymerase {ECO:0000256|RuleBase:RU004460};
KW   Hydrolase {ECO:0000256|SAAS:SAAS00427537};
KW   Nuclease {ECO:0000256|SAAS:SAAS00427491};
KW   Nucleotidyltransferase {ECO:0000256|RuleBase:RU004460,
KW   ECO:0000313|EMBL:ABE33161.1};
KW   Reference proteome {ECO:0000313|Proteomes:UP000001817};
KW   Transferase {ECO:0000256|RuleBase:RU004460,
KW   ECO:0000313|EMBL:ABE33161.1}.
FT   DOMAIN        9    264       53EXOc. {ECO:0000259|SMART:SM00475}.
FT   DOMAIN      316    502       3'-5' exonuclease. {ECO:0000259|SMART:
FT                                SM00474}.
FT   DOMAIN      671    877       POLAc. {ECO:0000259|SMART:SM00482}.
FT   COILED      534    554       {ECO:0000256|SAM:Coils}.
SQ   SEQUENCE   913 AA;  101216 MW;  785AF108FC20FD7F CRC64;
     MPEERSLEGK TLLLVDGSSY LYRAYHAMPD LRGPDGGPTG ALYGMINMLR RMRKEVTAEY
     SACVFDAKGK TFRDDWYADY KANRPSMPDD LSRQIEPIHV AVRALGWPLL MIEGVEADDV
     IGTLSTEAEK RGMNVIVSTG DKDLAQLVSD HVTLINTMTN EKLDRAGVLA KFGVPPERII
     DYLSLVGDTV DNVPGVEKCG PKTAVKWLTQ FDSLDGIVAH ADEIKGAVGD NLRRALDFLP
     MAKKLVTVER NCDLTDHIVS IGESLQSQPE SRAELREVFT RHGFKTWLRE VEIADAVEGP
     ETDVPPALSV DHERHYDTVQ TWEQFDAWLG KINAAELTSF DTETTSLDPM IAQIVGLSLS
     VEAGRAAYVP LAHRGPDAPV QLPRDEVLAR LKPWLESAGH KKVGQHLKYD EQVLANYGIE
     MRGIEHDTLL QSYVVESHRT HDMDSLALRH LGVKTIKYEE VAGKGAGQIG FDEVALDKAA
     EYAAEDADIT LRLHQVLYPQ VAAEKTLQYV YHDIELPTSR VLRKMERTGV LIDSEKLRQQ
     SSEIATRLIQ LESEAYVLAG GEFNLGSPKQ IGQIFFEKLE LPVVKKTPSG APSTDEEVLQ
     KLAEDFPLPK ILLEHRGLSK LKSTYTDKLP RMVNATTGRV HTNYAQAVAV TGRLASNDPN
     LQNIPVRTGE GRRIREAFIA PAGHKLVSAD YSQIELRIMA HISGDESLLH SFSQGEDIHR
     ATAAEIFSVT PLEVSNDQRR VAKVINFGLI YGMSSFGLAS NLGITRDAAK LYIDRYFARY
     PGVARYMDET RVSAKAKGYV ETVFGRRLWL PEINGGNGPR RQAAERAAIN APMQGTAADL
     IKLSMIAVQK WIEESKIGTR MIMQVHDELI LEVPDAELSD VRKRLPELMC GVADLKVPLV
     AEVGAGLNWE EAH
//

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