(data stored in ACNUC7421 zone)

SWISSPROT: RCOM2_PARXL

ID   RCOM2_PARXL             Reviewed;         267 AA.
AC   Q13IY4;
DT   14-OCT-2008, integrated into UniProtKB/Swiss-Prot.
DT   22-AUG-2006, sequence version 1.
DT   08-MAY-2019, entry version 75.
DE   RecName: Full=Heme-containing CO-sensing transcriptional regulator RcoM 2;
DE   AltName: Full=Regulator of CO metabolism 2;
DE            Short=RCOM-2;
GN   Name=rcoM2; OrderedLocusNames=Bxeno_C0027; ORFNames=Bxe_C0028;
OS   Paraburkholderia xenovorans (strain LB400).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Burkholderiaceae; Paraburkholderia.
OX   NCBI_TaxID=266265;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=LB400;
RX   PubMed=17030797; DOI=10.1073/pnas.0606924103;
RA   Chain P.S.G., Denef V.J., Konstantinidis K.T., Vergez L.M., Agullo L.,
RA   Reyes V.L., Hauser L., Cordova M., Gomez L., Gonzalez M., Land M.,
RA   Lao V., Larimer F., LiPuma J.J., Mahenthiralingam E., Malfatti S.A.,
RA   Marx C.J., Parnell J.J., Ramette A., Richardson P., Seeger M.,
RA   Smith D., Spilker T., Sul W.J., Tsoi T.V., Ulrich L.E., Zhulin I.B.,
RA   Tiedje J.M.;
RT   "Burkholderia xenovorans LB400 harbors a multi-replicon, 9.73-Mbp
RT   genome shaped for versatility.";
RL   Proc. Natl. Acad. Sci. U.S.A. 103:15280-15287(2006).
RN   [2]
RP   FUNCTION IN REGULATION OF AEROBIC CO OXIDATION.
RX   PubMed=18326575; DOI=10.1128/JB.00033-08;
RA   Kerby R.L., Youn H., Roberts G.P.;
RT   "RcoM: a new single-component transcriptional regulator of CO
RT   metabolism in bacteria.";
RL   J. Bacteriol. 190:3336-3343(2008).
RN   [3]
RP   CHARACTERIZATION, AND HEME COFACTOR.
RX   PubMed=18672900; DOI=10.1021/bi800486x;
RA   Marvin K.A., Kerby R.L., Youn H., Roberts G.P., Burstyn J.N.;
RT   "The transcription regulator RcoM-2 from Burkholderia xenovorans is a
RT   cysteine-ligated hemoprotein that undergoes a redox-mediated ligand
RT   switch.";
RL   Biochemistry 47:9016-9028(2008).
CC   -!- FUNCTION: One-component, b-type heme-containing aerobic sensor and
CC       transcriptional regulator that responds to CO by activating the
CC       expression of the oxidation operon cox.
CC       {ECO:0000269|PubMed:18326575}.
CC   -!- COFACTOR:
CC       Name=heme; Xref=ChEBI:CHEBI:30413;
CC       Note=Binds 1 heme group per subunit.;
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC   -!- DOMAIN: The N-terminal sensor (heme-containing) domain is
CC       covalently linked to the C-terminal, DNA-binding response domain.
CC   -!- DOMAIN: Binding of an external ligand to the heme located in the
CC       N-terminal sensory domain displaces the Met-104 distal heme
CC       ligand, triggering a conformational change that activates the C-
CC       terminal DNA-binding domain.
CC   -!- MISCELLANEOUS: This protein undergoes a ligand switch upon
CC       reduction; the heme distal ligand is coordinated by a Cys residue
CC       in the inactive Fe(3+) (ferric) form, by Met-104 in the inactive
CC       Fe(2+) (ferrous) form, and by CO in the CO-bound active form.
DR   EMBL; CP000272; ABE35955.1; -; Genomic_DNA.
DR   RefSeq; WP_011493215.1; NZ_CP008761.1.
DR   SMR; Q13IY4; -.
DR   STRING; 266265.Bxe_C0028; -.
DR   EnsemblBacteria; ABE35955; ABE35955; Bxe_C0028.
DR   GeneID; 4009816; -.
DR   KEGG; bxb:DR64_8397; -.
DR   KEGG; bxe:Bxe_C0028; -.
DR   PATRIC; fig|266265.5.peg.7807; -.
DR   eggNOG; ENOG4105SWR; Bacteria.
DR   eggNOG; ENOG4111VFD; LUCA.
DR   HOGENOM; HOG000004381; -.
DR   KO; K21696; -.
DR   OMA; IFGHIPR; -.
DR   OrthoDB; 1958442at2; -.
DR   BioCyc; BXEN266265:BXE_RS36890-MONOMER; -.
DR   Proteomes; UP000001817; Chromosome 3.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   InterPro; IPR007492; LytTR_DNA-bd_dom.
DR   InterPro; IPR035965; PAS-like_dom_sf.
DR   Pfam; PF04397; LytTR; 1.
DR   SMART; SM00850; LytTR; 1.
DR   SUPFAM; SSF55785; SSF55785; 1.
DR   PROSITE; PS50930; HTH_LYTTR; 1.
PE   1: Evidence at protein level;
DR   PRODOM; Q13IY4.
DR   SWISS-2DPAGE; Q13IY4.
KW   Activator; Complete proteome; Cytoplasm; DNA-binding; Heme; Iron;
KW   Metal-binding; Reference proteome; Repeat; Transcription;
KW   Transcription regulation.
FT   CHAIN         1    267       Heme-containing CO-sensing
FT                                transcriptional regulator RcoM 2.
FT                                /FTId=PRO_0000352734.
FT   DOMAIN       15     86       PAS.
FT   DOMAIN      161    266       HTH LytTR-type. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00112}.
FT   METAL        74     74       Iron (heme axial ligand).
FT   METAL       104    104       Iron (heme axial ligand); alternate.
SQ   SEQUENCE   267 AA;  29471 MW;  8E2A18388C715776 CRC64;
     MKSSESAAAT ASERRAETFQ HKLEQFNPGI VWLDPQGHVS AFNDVALHIL GPAGEQSLGV
     AQDHLFGIDV VQLHPEKSRD KLRFLLQSRD AGGCPVRSPP PVAMMINIPD RILMIKVSKM
     TGAAGTCGSC MIFYDVTDLT TEPSSQPAGA SVPAPRRLFK IPVYRKSRVI LIDLKDIVRF
     QGDGHYTTIV TKDERYLSNL SLADLELRLD SSVYLRVHRS HIVSLPYAVE LVKLDESVNL
     VMDDAEQTQV PVSRSRTAQL KELLGVV
//

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