(data stored in ACNUC7421 zone)

SWISSPROT: TRUA_RHOPS

ID   TRUA_RHOPS              Reviewed;         245 AA.
AC   Q13F20;
DT   24-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT   31-OCT-2006, sequence version 1.
DT   08-MAY-2019, entry version 82.
DE   RecName: Full=tRNA pseudouridine synthase A {ECO:0000255|HAMAP-Rule:MF_00171};
DE            EC=5.4.99.12 {ECO:0000255|HAMAP-Rule:MF_00171};
DE   AltName: Full=tRNA pseudouridine(38-40) synthase {ECO:0000255|HAMAP-Rule:MF_00171};
DE   AltName: Full=tRNA pseudouridylate synthase I {ECO:0000255|HAMAP-Rule:MF_00171};
DE   AltName: Full=tRNA-uridine isomerase I {ECO:0000255|HAMAP-Rule:MF_00171};
GN   Name=truA {ECO:0000255|HAMAP-Rule:MF_00171};
GN   OrderedLocusNames=RPD_0079;
OS   Rhodopseudomonas palustris (strain BisB5).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales;
OC   Bradyrhizobiaceae; Rhodopseudomonas.
OX   NCBI_TaxID=316057;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=BisB5;
RG   US DOE Joint Genome Institute;
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA   Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H.,
RA   Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J.,
RA   Larimer F., Land M., Hauser L., Pelletier D.A., Kyrpides N.,
RA   Lykidis A., Oda Y., Harwood C.S., Richardson P.;
RT   "Complete sequence of Rhodopseudomonas palustris BisB5.";
RL   Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Formation of pseudouridine at positions 38, 39 and 40 in
CC       the anticodon stem and loop of transfer RNAs. {ECO:0000255|HAMAP-
CC       Rule:MF_00171}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=tRNA uridine(38-40) = tRNA pseudouridine(38-40);
CC         Xref=Rhea:RHEA:22376, Rhea:RHEA-COMP:10085, Rhea:RHEA-
CC         COMP:10087, ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; EC=5.4.99.12;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00171};
CC   -!- SUBUNIT: Homodimer. {ECO:0000255|HAMAP-Rule:MF_00171}.
CC   -!- SIMILARITY: Belongs to the tRNA pseudouridine synthase TruA
CC       family. {ECO:0000255|HAMAP-Rule:MF_00171}.
DR   EMBL; CP000283; ABE37319.1; -; Genomic_DNA.
DR   RefSeq; WP_011500512.1; NC_007958.1.
DR   SMR; Q13F20; -.
DR   STRING; 316057.RPD_0079; -.
DR   EnsemblBacteria; ABE37319; ABE37319; RPD_0079.
DR   KEGG; rpd:RPD_0079; -.
DR   eggNOG; ENOG4105DI7; Bacteria.
DR   eggNOG; COG0101; LUCA.
DR   HOGENOM; HOG000248674; -.
DR   KO; K06173; -.
DR   OMA; FLYGMVR; -.
DR   OrthoDB; 1075262at2; -.
DR   BioCyc; RPAL316057:RPD_RS00395-MONOMER; -.
DR   Proteomes; UP000001818; Chromosome.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0106029; F:tRNA pseudouridine synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0031119; P:tRNA pseudouridine synthesis; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.30.70.660; -; 1.
DR   HAMAP; MF_00171; TruA; 1.
DR   InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR   InterPro; IPR001406; PsdUridine_synth_TruA.
DR   InterPro; IPR020097; PsdUridine_synth_TruA_a/b_dom.
DR   InterPro; IPR020095; PsdUridine_synth_TruA_C.
DR   PANTHER; PTHR11142; PTHR11142; 1.
DR   Pfam; PF01416; PseudoU_synth_1; 2.
DR   PIRSF; PIRSF001430; tRNA_psdUrid_synth; 1.
DR   SUPFAM; SSF55120; SSF55120; 1.
DR   TIGRFAMs; TIGR00071; hisT_truA; 1.
PE   3: Inferred from homology;
DR   PRODOM; Q13F20.
DR   SWISS-2DPAGE; Q13F20.
KW   Complete proteome; Isomerase; tRNA processing.
FT   CHAIN         1    245       tRNA pseudouridine synthase A.
FT                                /FTId=PRO_1000097776.
FT   ACT_SITE     52     52       Nucleophile. {ECO:0000255|HAMAP-
FT                                Rule:MF_00171}.
FT   BINDING     111    111       Substrate. {ECO:0000255|HAMAP-
FT                                Rule:MF_00171}.
SQ   SEQUENCE   245 AA;  26911 MW;  95B5E258B99F4075 CRC64;
     MPRYKLTIEY DGAPFCGWQL QPVLPSVQGA LEAAALATCG EAVRVHGAGR TDAGVHALGQ
     VAHLDIAKPF RADRLRDALN AHVRPYPVAV LSTEIVPDSF EARFSAIRRH YRYRIVNRRS
     NLALEIGKVW RVPKPLDTDA MHRAAQVLIG KHDFTTFRDT ECQAASPEKT LDVLDVVRSG
     DCVDIITSAR SYLHSQVRSM VGSLVWVGEG RWSADDLAAA LAARRRSACG PVAPPDGLYL
     VKVDY
//

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