(data stored in ACNUC9543 zone)

SWISSPROT: A0A0H2Y2H8_YERPA

ID   A0A0H2Y2H8_YERPA        Unreviewed;       131 AA.
AC   A0A0H2Y2H8;
DT   16-SEP-2015, integrated into UniProtKB/TrEMBL.
DT   16-SEP-2015, sequence version 1.
DT   08-MAY-2019, entry version 23.
DE   RecName: Full=Sulfur carrier protein TusA {ECO:0000256|HAMAP-Rule:MF_00413, ECO:0000256|SAAS:SAAS01077704};
DE   AltName: Full=Sulfur mediator TusA {ECO:0000256|HAMAP-Rule:MF_00413};
DE   AltName: Full=Sulfur transfer protein TusA {ECO:0000256|HAMAP-Rule:MF_00413};
DE   AltName: Full=tRNA 2-thiouridine synthesizing protein A {ECO:0000256|HAMAP-Rule:MF_00413};
GN   Name=tusA {ECO:0000256|HAMAP-Rule:MF_00413};
GN   OrderedLocusNames=YPA_0201 {ECO:0000313|EMBL:ABG12170.1};
OS   Yersinia pestis bv. Antiqua (strain Antiqua).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Yersiniaceae; Yersinia.
OX   NCBI_TaxID=360102 {ECO:0000313|EMBL:ABG12170.1, ECO:0000313|Proteomes:UP000001971};
RN   [1] {ECO:0000313|EMBL:ABG12170.1, ECO:0000313|Proteomes:UP000001971}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Antiqua {ECO:0000313|EMBL:ABG12170.1,
RC   ECO:0000313|Proteomes:UP000001971};
RX   PubMed=16740952; DOI=10.1128/JB.00124-06;
RA   Chain P.S., Hu P., Malfatti S.A., Radnedge L., Larimer F.,
RA   Vergez L.M., Worsham P., Chu M.C., Andersen G.L.;
RT   "Complete genome sequence of Yersinia pestis strains Antiqua and
RT   Nepal516: evidence of gene reduction in an emerging pathogen.";
RL   J. Bacteriol. 188:4453-4463(2006).
CC   -!- FUNCTION: Sulfur carrier protein involved in sulfur trafficking in
CC       the cell. Part of a sulfur-relay system required for 2-thiolation
CC       during synthesis of 2-thiouridine of the modified wobble base 5-
CC       methylaminomethyl-2-thiouridine (mnm(5)s(2)U) in tRNA. Interacts
CC       with IscS and stimulates its cysteine desulfurase activity.
CC       Accepts an activated sulfur from IscS, which is then transferred
CC       to TusD, and thus determines the direction of sulfur flow from
CC       IscS to 2-thiouridine formation. Also appears to be involved in
CC       sulfur transfer for the biosynthesis of molybdopterin.
CC       {ECO:0000256|HAMAP-Rule:MF_00413}.
CC   -!- PATHWAY: tRNA modification. {ECO:0000256|HAMAP-Rule:MF_00413}.
CC   -!- SUBUNIT: Interacts with IscS. {ECO:0000256|HAMAP-Rule:MF_00413}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00413,
CC       ECO:0000256|SAAS:SAAS00345499}.
CC   -!- SIMILARITY: Belongs to the sulfur carrier protein TusA family.
CC       {ECO:0000256|HAMAP-Rule:MF_00413, ECO:0000256|SAAS:SAAS01077701}.
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DR   EMBL; CP000308; ABG12170.1; -; Genomic_DNA.
DR   EnsemblBacteria; ABG12170; ABG12170; YPA_0201.
DR   KEGG; ypa:YPA_0201; -.
DR   KO; K04085; -.
DR   OMA; DAQGLRC; -.
DR   Proteomes; UP000001971; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0097163; F:sulfur carrier activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0002143; P:tRNA wobble position uridine thiolation; IEA:InterPro.
DR   CDD; cd03423; SirA; 1.
DR   Gene3D; 3.30.110.40; -; 1.
DR   HAMAP; MF_00413; Thiourid_synth_A; 1.
DR   InterPro; IPR022931; Sulphur_carrier_TusA.
DR   InterPro; IPR001455; TusA-like.
DR   InterPro; IPR036868; TusA-like_sf.
DR   Pfam; PF01206; TusA; 1.
DR   SUPFAM; SSF64307; SSF64307; 1.
DR   PROSITE; PS01148; UPF0033; 1.
PE   3: Inferred from homology;
DR   PRODOM; A0A0H2Y2H8.
DR   SWISS-2DPAGE; A0A0H2Y2H8.
KW   Complete proteome {ECO:0000313|Proteomes:UP000001971};
KW   Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00413,
KW   ECO:0000256|SAAS:SAAS00016049};
KW   tRNA processing {ECO:0000256|HAMAP-Rule:MF_00413}.
FT   DOMAIN       59     83       UPF0033. {ECO:0000259|PROSITE:PS01148}.
FT   ACT_SITE     66     66       Cysteine persulfide intermediate.
FT                                {ECO:0000256|HAMAP-Rule:MF_00413}.
SQ   SEQUENCE   131 AA;  14893 MW;  B1D63F693B1FC949 CRC64;
     MVKLFAFVKP SDFVKLAAFV SLLTFYLSIK PLTDELLNRL LTNKRTAMTD IFANPDKTLD
     ALGLRCPEPV MMVRKTVRHM EEGQTLLIIA DDPATTRDIP GFCRFMDHQL LAQDTEQTPY
     RYLVRKGITA G
//

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