(data stored in ACNUC9543 zone)

SWISSPROT: QUEF_YERPA

ID   QUEF_YERPA              Reviewed;         281 AA.
AC   Q1CAP6;
DT   05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   11-JUL-2006, sequence version 1.
DT   08-MAY-2019, entry version 79.
DE   RecName: Full=NADPH-dependent 7-cyano-7-deazaguanine reductase {ECO:0000255|HAMAP-Rule:MF_00817};
DE            EC=1.7.1.13 {ECO:0000255|HAMAP-Rule:MF_00817};
DE   AltName: Full=7-cyano-7-carbaguanine reductase {ECO:0000255|HAMAP-Rule:MF_00817};
DE   AltName: Full=NADPH-dependent nitrile oxidoreductase {ECO:0000255|HAMAP-Rule:MF_00817};
DE   AltName: Full=PreQ(0) reductase {ECO:0000255|HAMAP-Rule:MF_00817};
GN   Name=queF {ECO:0000255|HAMAP-Rule:MF_00817};
GN   OrderedLocusNames=YPA_0508;
OS   Yersinia pestis bv. Antiqua (strain Antiqua).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Yersiniaceae; Yersinia.
OX   NCBI_TaxID=360102;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Antiqua;
RX   PubMed=16740952; DOI=10.1128/JB.00124-06;
RA   Chain P.S.G., Hu P., Malfatti S.A., Radnedge L., Larimer F.,
RA   Vergez L.M., Worsham P., Chu M.C., Andersen G.L.;
RT   "Complete genome sequence of Yersinia pestis strains Antiqua and
RT   Nepal516: evidence of gene reduction in an emerging pathogen.";
RL   J. Bacteriol. 188:4453-4463(2006).
CC   -!- FUNCTION: Catalyzes the NADPH-dependent reduction of 7-cyano-7-
CC       deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1).
CC       {ECO:0000255|HAMAP-Rule:MF_00817}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=7-aminomethyl-7-carbaguanine + 2 NADP(+) = 7-cyano-7-
CC         deazaguanine + 3 H(+) + 2 NADPH; Xref=Rhea:RHEA:13409,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:45075, ChEBI:CHEBI:57783,
CC         ChEBI:CHEBI:58349, ChEBI:CHEBI:58703; EC=1.7.1.13;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00817};
CC   -!- PATHWAY: tRNA modification; tRNA-queuosine biosynthesis.
CC       {ECO:0000255|HAMAP-Rule:MF_00817}.
CC   -!- SUBUNIT: Homodimer. {ECO:0000255|HAMAP-Rule:MF_00817}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00817}.
CC   -!- SIMILARITY: Belongs to the GTP cyclohydrolase I family. QueF type
CC       2 subfamily. {ECO:0000255|HAMAP-Rule:MF_00817}.
DR   EMBL; CP000308; ABG12476.1; -; Genomic_DNA.
DR   RefSeq; WP_002212122.1; NZ_CP009906.1.
DR   SMR; Q1CAP6; -.
DR   EnsemblBacteria; ABG12476; ABG12476; YPA_0508.
DR   KEGG; ypa:YPA_0508; -.
DR   HOGENOM; HOG000273755; -.
DR   KO; K06879; -.
DR   OMA; QCVERIY; -.
DR   UniPathway; UPA00392; -.
DR   Proteomes; UP000001971; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0046857; F:oxidoreductase activity, acting on other nitrogenous compounds as donors, with NAD or NADP as acceptor; IEA:InterPro.
DR   GO; GO:0033739; F:preQ1 synthase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0008616; P:queuosine biosynthetic process; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00817; QueF_type2; 1.
DR   InterPro; IPR029500; QueF.
DR   InterPro; IPR029139; QueF_N.
DR   InterPro; IPR016428; QueF_type2.
DR   Pfam; PF14489; QueF; 1.
DR   Pfam; PF14819; QueF_N; 1.
DR   PIRSF; PIRSF004750; Nitrile_oxidored_YqcD_prd; 1.
DR   TIGRFAMs; TIGR03138; QueF; 1.
PE   3: Inferred from homology;
DR   PRODOM; Q1CAP6.
DR   SWISS-2DPAGE; Q1CAP6.
KW   Complete proteome; Cytoplasm; NADP; Oxidoreductase;
KW   Queuosine biosynthesis.
FT   CHAIN         1    281       NADPH-dependent 7-cyano-7-deazaguanine
FT                                reductase.
FT                                /FTId=PRO_1000062370.
FT   NP_BIND      90     91       NADPH. {ECO:0000255|HAMAP-Rule:MF_00817}.
FT   NP_BIND     257    258       NADPH. {ECO:0000255|HAMAP-Rule:MF_00817}.
FT   REGION       88     90       Substrate binding. {ECO:0000255|HAMAP-
FT                                Rule:MF_00817}.
FT   REGION      228    229       Substrate binding. {ECO:0000255|HAMAP-
FT                                Rule:MF_00817}.
FT   ACT_SITE    189    189       Thioimide intermediate.
FT                                {ECO:0000255|HAMAP-Rule:MF_00817}.
FT   ACT_SITE    196    196       Proton donor. {ECO:0000255|HAMAP-
FT                                Rule:MF_00817}.
SQ   SEQUENCE   281 AA;  32082 MW;  A08B7182F11E5CCA CRC64;
     MSSYQNHKAL AELTLGKPTA YCDYYDATLL QAVPRSMNRE PLGLYPDNLP FHGADIWTLY
     ELSWLNSNGL PQVAVGEISL NADSINLIES KSFKLYLNSF NQTIFADKES VRMTLQRDLA
     ACAQGNVSVA LYDLDEITGQ PISNFNGECL DKQDIRIDSY EFNADYLQGA AGKDHVEESL
     VSHLLKSNCL ITHQPDWGSV QIHYRGPQID HEALLRYLVS FRHHNEFHEQ CVERIFNDIM
     RFCQPETLTV YARYTRRGGL DINPWRSNTD FVPLTGRLAR Q
//

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