(data stored in ACNUC7421 zone)

SWISSPROT: A0A0H2ZKE4_PSEAB

ID   A0A0H2ZKE4_PSEAB        Unreviewed;       456 AA.
AC   A0A0H2ZKE4;
DT   16-SEP-2015, integrated into UniProtKB/TrEMBL.
DT   16-SEP-2015, sequence version 1.
DT   08-MAY-2019, entry version 18.
DE   RecName: Full=Guanine deaminase {ECO:0000256|RuleBase:RU366009};
DE            Short=Guanase {ECO:0000256|RuleBase:RU366009};
DE            EC=3.5.4.3 {ECO:0000256|RuleBase:RU366009};
DE   AltName: Full=Guanine aminohydrolase {ECO:0000256|RuleBase:RU366009};
GN   Name=guaD {ECO:0000313|EMBL:ABJ15091.1};
GN   OrderedLocusNames=PA14_01660 {ECO:0000313|EMBL:ABJ15091.1};
OS   Pseudomonas aeruginosa (strain UCBPP-PA14).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=208963 {ECO:0000313|EMBL:ABJ15091.1, ECO:0000313|Proteomes:UP000000653};
RN   [1] {ECO:0000313|EMBL:ABJ15091.1, ECO:0000313|Proteomes:UP000000653}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=UCBPP-PA14 {ECO:0000313|EMBL:ABJ15091.1,
RC   ECO:0000313|Proteomes:UP000000653};
RX   PubMed=17038190; DOI=10.1186/gb-2006-7-10-r90;
RA   Lee D.G., Urbach J.M., Wu G., Liberati N.T., Feinbaum R.L., Miyata S.,
RA   Diggins L.T., He J., Saucier M., Deziel E., Friedman L., Li L.,
RA   Grills G., Montgomery K., Kucherlapati R., Rahme L.G., Ausubel F.M.;
RT   "Genomic analysis reveals that Pseudomonas aeruginosa virulence is
RT   combinatorial.";
RL   Genome Biol. 7:R90.1-R90.14(2006).
CC   -!- FUNCTION: Catalyzes the hydrolytic deamination of guanine,
CC       producing xanthine and ammonia. {ECO:0000256|RuleBase:RU366009}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=guanine + H(+) + H2O = NH4(+) + xanthine;
CC         Xref=Rhea:RHEA:14665, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:16235, ChEBI:CHEBI:17712, ChEBI:CHEBI:28938;
CC         EC=3.5.4.3; Evidence={ECO:0000256|RuleBase:RU366009};
CC   -!- COFACTOR:
CC       Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC         Evidence={ECO:0000256|RuleBase:RU366009};
CC       Note=Binds 1 zinc ion per subunit.
CC       {ECO:0000256|RuleBase:RU366009};
CC   -!- PATHWAY: Purine metabolism; guanine degradation; xanthine from
CC       guanine: step 1/1. {ECO:0000256|RuleBase:RU366009}.
CC   -!- SIMILARITY: Belongs to the metallo-dependent hydrolases
CC       superfamily. ATZ/TRZ family. {ECO:0000256|RuleBase:RU366009}.
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DR   EMBL; CP000438; ABJ15091.1; -; Genomic_DNA.
DR   RefSeq; WP_003137018.1; NC_008463.1.
DR   EnsemblBacteria; ABJ15091; ABJ15091; PA14_01660.
DR   KEGG; pau:PA14_01660; -.
DR   KO; K01487; -.
DR   OMA; IIASWGK; -.
DR   BioCyc; PAER208963:G1G74-139-MONOMER; -.
DR   UniPathway; UPA00603; UER00660.
DR   Proteomes; UP000000653; Chromosome.
DR   GO; GO:0008892; F:guanine deaminase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0006147; P:guanine catabolic process; IEA:UniProtKB-UniRule.
DR   CDD; cd01303; GDEase; 1.
DR   Gene3D; 2.30.40.10; -; 1.
DR   InterPro; IPR006680; Amidohydro-rel.
DR   InterPro; IPR014311; Guanine_deaminase.
DR   InterPro; IPR011059; Metal-dep_hydrolase_composite.
DR   InterPro; IPR032466; Metal_Hydrolase.
DR   PANTHER; PTHR11271:SF6; PTHR11271:SF6; 1.
DR   Pfam; PF01979; Amidohydro_1; 1.
DR   SUPFAM; SSF51338; SSF51338; 1.
DR   SUPFAM; SSF51556; SSF51556; 1.
DR   TIGRFAMs; TIGR02967; guan_deamin; 1.
PE   3: Inferred from homology;
DR   PRODOM; A0A0H2ZKE4.
DR   SWISS-2DPAGE; A0A0H2ZKE4.
KW   Complete proteome {ECO:0000313|Proteomes:UP000000653};
KW   Hydrolase {ECO:0000256|RuleBase:RU366009};
KW   Metal-binding {ECO:0000256|RuleBase:RU366009};
KW   Zinc {ECO:0000256|RuleBase:RU366009}.
FT   DOMAIN       91    450       Amidohydro-rel. {ECO:0000259|Pfam:
FT                                PF01979}.
SQ   SEQUENCE   456 AA;  49656 MW;  C4105C8809C5074C CRC64;
     MNDAVPAGNF VPPLVLRSDH GGSAMSSSAH RGRILHFLGD PAKLGDKAWE YFEDGLLWIE
     HGHVRALDHA AYLLPQLPAD LPLEEHPQRL LLPGFVDCHV HYPQLGVIAS YGTQLLDWLE
     THTFPAEQRF ADAGYAAAQA ELFLDELLRH GTTTALVFGT VHAVSAEAFF QAAQKRRLRM
     IAGKVLMDRN APPALCDTAA SGYAESRALI ERWHGNGRLQ YAVTPRFAPT SSPEQLAAAA
     RLLDEYPGVY LHTHLSENLK EVAWVGELFP QAQDYLDVYH RAGLVGERSV FAHGIHLSER
     ECRCLAHKNA ALAHCPSSNL FIGSGLFDLG RAQQYGIRVG IGSDVGGGTS LSLLANLADA
     YKIQQLRGTS LDPFQALYLA TLGGARALDL DGLVGNFLPG READFVALDL AATPMIAQRM
     EHARGLADTL FVLNTLGDDR AVAETWVMGE RRHAKG
//

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