(data stored in ACNUC7421 zone)

SWISSPROT: A0A0H2ZJS9_PSEAB

ID   A0A0H2ZJS9_PSEAB        Unreviewed;       329 AA.
AC   A0A0H2ZJS9;
DT   16-SEP-2015, integrated into UniProtKB/TrEMBL.
DT   16-SEP-2015, sequence version 1.
DT   08-MAY-2019, entry version 27.
DE   RecName: Full=Sulfate/thiosulfate import ATP-binding protein CysA {ECO:0000256|HAMAP-Rule:MF_01701};
DE            EC=7.3.2.3 {ECO:0000256|HAMAP-Rule:MF_01701};
DE   AltName: Full=Sulfate-transporting ATPase {ECO:0000256|HAMAP-Rule:MF_01701};
GN   Name=cysA {ECO:0000256|HAMAP-Rule:MF_01701,
GN   ECO:0000313|EMBL:ABJ15243.1};
GN   OrderedLocusNames=PA14_03650 {ECO:0000313|EMBL:ABJ15243.1};
OS   Pseudomonas aeruginosa (strain UCBPP-PA14).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=208963 {ECO:0000313|EMBL:ABJ15243.1, ECO:0000313|Proteomes:UP000000653};
RN   [1] {ECO:0000313|EMBL:ABJ15243.1, ECO:0000313|Proteomes:UP000000653}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=UCBPP-PA14 {ECO:0000313|EMBL:ABJ15243.1,
RC   ECO:0000313|Proteomes:UP000000653};
RX   PubMed=17038190; DOI=10.1186/gb-2006-7-10-r90;
RA   Lee D.G., Urbach J.M., Wu G., Liberati N.T., Feinbaum R.L., Miyata S.,
RA   Diggins L.T., He J., Saucier M., Deziel E., Friedman L., Li L.,
RA   Grills G., Montgomery K., Kucherlapati R., Rahme L.G., Ausubel F.M.;
RT   "Genomic analysis reveals that Pseudomonas aeruginosa virulence is
RT   combinatorial.";
RL   Genome Biol. 7:R90.1-R90.14(2006).
CC   -!- FUNCTION: Part of the ABC transporter complex CysAWTP involved in
CC       sulfate/thiosulfate import. Responsible for energy coupling to the
CC       transport system. {ECO:0000256|HAMAP-Rule:MF_01701,
CC       ECO:0000256|SAAS:SAAS00360330}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O + sulfate(out) = ADP + H(+) + phosphate +
CC         sulfate(in); Xref=Rhea:RHEA:10192, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:16189, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=7.3.2.3;
CC         Evidence={ECO:0000256|HAMAP-Rule:MF_01701,
CC         ECO:0000256|SAAS:SAAS01131877};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O + thiosulfate(out) = ADP + H(+) + phosphate +
CC         thiosulfate(in); Xref=Rhea:RHEA:29871, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:33542,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=7.3.2.3;
CC         Evidence={ECO:0000256|HAMAP-Rule:MF_01701,
CC         ECO:0000256|SAAS:SAAS01131887};
CC   -!- SUBUNIT: The complex is composed of two ATP-binding proteins
CC       (CysA), two transmembrane proteins (CysT and CysW) and a solute-
CC       binding protein (CysP). {ECO:0000256|HAMAP-Rule:MF_01701,
CC       ECO:0000256|SAAS:SAAS00360324}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000256|HAMAP-
CC       Rule:MF_01701}; Peripheral membrane protein {ECO:0000256|HAMAP-
CC       Rule:MF_01701}.
CC   -!- SIMILARITY: Belongs to the ABC transporter superfamily.
CC       Sulfate/tungstate importer (TC 3.A.1.6) family.
CC       {ECO:0000256|HAMAP-Rule:MF_01701, ECO:0000256|SAAS:SAAS00552311}.
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DR   EMBL; CP000438; ABJ15243.1; -; Genomic_DNA.
DR   RefSeq; WP_003084237.1; NC_008463.1.
DR   SMR; A0A0H2ZJS9; -.
DR   EnsemblBacteria; ABJ15243; ABJ15243; PA14_03650.
DR   KEGG; pau:PA14_03650; -.
DR   KO; K02045; -.
DR   OMA; MEVRVQN; -.
DR   BioCyc; PAER208963:G1G74-307-MONOMER; -.
DR   Proteomes; UP000000653; Chromosome.
DR   GO; GO:0043190; C:ATP-binding cassette (ABC) transporter complex; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0015419; F:ATPase-coupled sulfate transmembrane transporter activity; IEA:UniProtKB-UniRule.
DR   CDD; cd03296; ABC_CysA_sulfate_importer; 1.
DR   InterPro; IPR003593; AAA+_ATPase.
DR   InterPro; IPR014769; ABC_CysA_ATP-bd_C.
DR   InterPro; IPR003439; ABC_transporter-like.
DR   InterPro; IPR017871; ABC_transporter_CS.
DR   InterPro; IPR008995; Mo/tungstate-bd_C_term_dom.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR005666; Sulph_transpt1.
DR   InterPro; IPR024765; TOBE-like.
DR   Pfam; PF00005; ABC_tran; 1.
DR   Pfam; PF12857; TOBE_3; 1.
DR   SMART; SM00382; AAA; 1.
DR   SUPFAM; SSF50331; SSF50331; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR00968; 3a0106s01; 1.
DR   PROSITE; PS00211; ABC_TRANSPORTER_1; 1.
DR   PROSITE; PS50893; ABC_TRANSPORTER_2; 1.
DR   PROSITE; PS51237; CYSA; 1.
PE   3: Inferred from homology;
DR   PRODOM; A0A0H2ZJS9.
DR   SWISS-2DPAGE; A0A0H2ZJS9.
KW   ATP-binding {ECO:0000256|HAMAP-Rule:MF_01701, ECO:0000256|PROSITE-
KW   ProRule:PRU00434, ECO:0000256|SAAS:SAAS00237035};
KW   Cell inner membrane {ECO:0000256|HAMAP-Rule:MF_01701};
KW   Cell membrane {ECO:0000256|HAMAP-Rule:MF_01701,
KW   ECO:0000256|SAAS:SAAS00437629};
KW   Complete proteome {ECO:0000313|Proteomes:UP000000653};
KW   Membrane {ECO:0000256|HAMAP-Rule:MF_01701,
KW   ECO:0000256|SAAS:SAAS00437643};
KW   Nucleotide-binding {ECO:0000256|HAMAP-Rule:MF_01701,
KW   ECO:0000256|PROSITE-ProRule:PRU00434, ECO:0000256|SAAS:SAAS00452314};
KW   Sulfate transport {ECO:0000256|HAMAP-Rule:MF_01701,
KW   ECO:0000256|SAAS:SAAS00437635};
KW   Translocase {ECO:0000256|HAMAP-Rule:MF_01701,
KW   ECO:0000256|SAAS:SAAS01131881};
KW   Transport {ECO:0000256|HAMAP-Rule:MF_01701,
KW   ECO:0000256|SAAS:SAAS00237071}.
FT   DOMAIN        3    237       ABC transporter. {ECO:0000259|PROSITE:
FT                                PS50893}.
FT   DOMAIN      197    322       CYSA. {ECO:0000259|PROSITE:PS51237}.
FT   NP_BIND      35     42       ATP. {ECO:0000256|PROSITE-ProRule:
FT                                PRU00434}.
SQ   SEQUENCE   329 AA;  36795 MW;  337C236518EF3FA5 CRC64;
     MSIEIRNVSK NFNAFKALDD INLDIQSGEL VALLGPSGCG KTTLLRIIAG LETPDAGNIV
     FHGEDVSQHD VRDRNVGFVF QHYALFRHMT VFDNVAFGLR MKPKGERPGE SAIKAKVHEL
     LNMVQLDWLA DRYPEQLSGG QRQRIALARA LAVEPKILLL DEPFGALDAK VRKELRRWLA
     RLHEEINLTS VFVTHDQEEA MEVADRIVVM NKGVIEQIGS PGEVYENPAS DFVYHFLGDS
     NRLQLGNDQH LLFRPHEVSL SRSAVAEHRA AEVRDIRPLG AITRVTLKVD GQDELIEAEV
     VKDHDSLAGL ARGETLYFKP KAFQPVANL
//

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