(data stored in ACNUC7421 zone)

SWISSPROT: SPUD_PSEAB

ID   SPUD_PSEAB              Reviewed;         367 AA.
AC   Q02UB7;
DT   07-JAN-2015, integrated into UniProtKB/Swiss-Prot.
DT   14-NOV-2006, sequence version 1.
DT   05-JUL-2017, entry version 58.
DE   RecName: Full=Putrescine-binding periplasmic protein SpuD;
DE   Flags: Precursor;
GN   Name=spuD; OrderedLocusNames=PA14_03920;
OS   Pseudomonas aeruginosa (strain UCBPP-PA14).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=208963;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=UCBPP-PA14;
RX   PubMed=17038190; DOI=10.1186/gb-2006-7-10-r90;
RA   Lee D.G., Urbach J.M., Wu G., Liberati N.T., Feinbaum R.L., Miyata S.,
RA   Diggins L.T., He J., Saucier M., Deziel E., Friedman L., Li L.,
RA   Grills G., Montgomery K., Kucherlapati R., Rahme L.G., Ausubel F.M.;
RT   "Genomic analysis reveals that Pseudomonas aeruginosa virulence is
RT   combinatorial.";
RL   Genome Biol. 7:R90.1-R90.14(2006).
RN   [2]
RP   IDENTIFICATION BY MASS SPECTROMETRY, AND SUBCELLULAR LOCATION.
RC   STRAIN=UCBPP-PA14;
RX   PubMed=24965220; DOI=10.1002/pmic.201400190;
RA   Ouidir T., Jarnier F., Cosette P., Jouenne T., Hardouin J.;
RT   "Extracellular Ser/Thr/Tyr phosphorylated proteins of Pseudomonas
RT   aeruginosa PA14 strain.";
RL   Proteomics 14:2017-2030(2014).
CC   -!- FUNCTION: Putrescine-binding protein probably required for
CC       putrescine uptake into cells. Binds putrescine with high affinity,
CC       spermidine with relatively low affinity. Does not bind cadaverine
CC       or spermine. Putrescine binding induces large inter-domain
CC       conformational changes. {ECO:0000250|UniProtKB:Q9I6J1}.
CC   -!- SUBCELLULAR LOCATION: Periplasm {ECO:0000255|PIRNR:PIRNR019574}.
CC       Secreted {ECO:0000269|PubMed:24965220}. Note=Could be present
CC       extracellularly due to cell outer membrane leakage. {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the bacterial solute-binding protein
CC       PotD/PotF family. {ECO:0000255|PIRNR:PIRNR019574}.
DR   EMBL; CP000438; ABJ15262.1; -; Genomic_DNA.
DR   RefSeq; WP_003084299.1; NC_008463.1.
DR   ProteinModelPortal; Q02UB7; -.
DR   SMR; Q02UB7; -.
DR   EnsemblBacteria; ABJ15262; ABJ15262; PA14_03920.
DR   KEGG; pau:PA14_03920; -.
DR   HOGENOM; HOG000263815; -.
DR   KO; K11073; -.
DR   OMA; FPPQSVM; -.
DR   Proteomes; UP000000653; Chromosome.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0042597; C:periplasmic space; IEA:UniProtKB-SubCell.
DR   GO; GO:0019808; F:polyamine binding; IEA:InterPro.
DR   GO; GO:0015846; P:polyamine transport; IEA:InterPro.
DR   InterPro; IPR001188; Sperm_putr-bd.
DR   PIRSF; PIRSF019574; Periplasmic_polyamine_BP; 1.
DR   PRINTS; PR00909; SPERMDNBNDNG.
PE   1: Evidence at protein level;
DR   PRODOM; Q02UB7.
DR   SWISS-2DPAGE; Q02UB7.
KW   Complete proteome; Disulfide bond; Periplasm; Secreted; Signal;
KW   Transport.
FT   SIGNAL        1     24       {ECO:0000255}.
FT   CHAIN        25    367       Putrescine-binding periplasmic protein
FT                                SpuD. {ECO:0000255}.
FT                                /FTId=PRO_0000431395.
FT   REGION       36     37       Putrescine-binding.
FT                                {ECO:0000250|UniProtKB:Q9I6J1}.
FT   BINDING     244    244       Putrescine.
FT                                {ECO:0000250|UniProtKB:Q9I6J1}.
FT   BINDING     275    275       Putrescine.
FT                                {ECO:0000250|UniProtKB:Q9I6J1}.
FT   DISULFID    173    236       {ECO:0000250|UniProtKB:Q9I6J1}.
SQ   SEQUENCE   367 AA;  40630 MW;  8734EE5F8F9BAF94 CRC64;
     MMKRFGKTLL ALTLAGSVAG MAQAADNKVL HVYNWSDYIA PDTLEKFTKE TGIKVVYDVY
     DSNEVLEAKL LAGKSGYDVV VPSNSFLAKQ IKAGVYQKLD KSKLPNWKNL NKDLMHTLEV
     SDPGNEHAIP YMWGTIGIGY NPDKVKAAFG DNAPVDSWDL VFKPENIQKL KQCGVSFLDS
     PTEILPAALH YLGYKPDTDN PKELKAAEEL FLKIRPYVTY FHSSKYISDL ANGNICVAIG
     YSGDIYQAKS RAEEAKNKVT VKYNIPKEGA GSFFDMVAIP KDAENTEGAL AFVNFLMKPE
     IMAEITDVVQ FPNGNAAATP LVSEAIRNDP GIYPSEEVMK KLYTFPDLPA KTQRAMTRSW
     TKIKSGK
//

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