(data stored in ACNUC7421 zone)

SWISSPROT: MNMG_SYNFM

ID   MNMG_SYNFM              Reviewed;         642 AA.
AC   A0LEF5;
DT   22-JUL-2008, integrated into UniProtKB/Swiss-Prot.
DT   12-DEC-2006, sequence version 1.
DT   08-MAY-2019, entry version 77.
DE   RecName: Full=tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG {ECO:0000255|HAMAP-Rule:MF_00129};
DE   AltName: Full=Glucose-inhibited division protein A {ECO:0000255|HAMAP-Rule:MF_00129};
GN   Name=mnmG {ECO:0000255|HAMAP-Rule:MF_00129};
GN   Synonyms=gidA {ECO:0000255|HAMAP-Rule:MF_00129};
GN   OrderedLocusNames=Sfum_0104;
OS   Syntrophobacter fumaroxidans (strain DSM 10017 / MPOB).
OC   Bacteria; Proteobacteria; Deltaproteobacteria; Syntrophobacterales;
OC   Syntrophobacteraceae; Syntrophobacter.
OX   NCBI_TaxID=335543;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 10017 / MPOB;
RG   US DOE Joint Genome Institute;
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA   Glavina del Rio T., Hammon N., Israni S., Pitluck S., Goltsman E.G.,
RA   Martinez M., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N.,
RA   Kim E., Boone D.R., Brockman F., Culley D., Ferry J., Gunsalus R.,
RA   McInerney M.J., Morrison M., Plugge C., Rohlin L., Scholten J.,
RA   Sieber J., Stams A.J.M., Worm P., Henstra A.M., Richardson P.;
RT   "Complete sequence of Syntrophobacter fumaroxidans MPOB.";
RL   Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: NAD-binding protein involved in the addition of a
CC       carboxymethylaminomethyl (cmnm) group at the wobble position (U34)
CC       of certain tRNAs, forming tRNA-cmnm(5)s(2)U34. {ECO:0000255|HAMAP-
CC       Rule:MF_00129}.
CC   -!- COFACTOR:
CC       Name=FAD; Xref=ChEBI:CHEBI:57692;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00129};
CC   -!- SUBUNIT: Homodimer. Heterotetramer of two MnmE and two MnmG
CC       subunits. {ECO:0000255|HAMAP-Rule:MF_00129}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00129}.
CC   -!- SIMILARITY: Belongs to the MnmG family. {ECO:0000255|HAMAP-
CC       Rule:MF_00129}.
DR   EMBL; CP000478; ABK15807.1; -; Genomic_DNA.
DR   RefSeq; WP_011696980.1; NC_008554.1.
DR   SMR; A0LEF5; -.
DR   STRING; 335543.Sfum_0104; -.
DR   EnsemblBacteria; ABK15807; ABK15807; Sfum_0104.
DR   KEGG; sfu:Sfum_0104; -.
DR   eggNOG; ENOG4107RE5; Bacteria.
DR   eggNOG; COG0445; LUCA.
DR   HOGENOM; HOG000201060; -.
DR   KO; K03495; -.
DR   OMA; FRPGYAI; -.
DR   OrthoDB; 146811at2; -.
DR   BioCyc; SFUM335543:G1G7I-110-MONOMER; -.
DR   Proteomes; UP000001784; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0050660; F:flavin adenine dinucleotide binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0002098; P:tRNA wobble uridine modification; IEA:InterPro.
DR   Gene3D; 3.50.50.60; -; 2.
DR   HAMAP; MF_00129; MnmG_GidA; 1.
DR   InterPro; IPR036188; FAD/NAD-bd_sf.
DR   InterPro; IPR026904; GidA-assoc_3.
DR   InterPro; IPR004416; MnmG.
DR   InterPro; IPR002218; MnmG-rel.
DR   InterPro; IPR020595; MnmG-rel_CS.
DR   PANTHER; PTHR11806; PTHR11806; 1.
DR   PANTHER; PTHR11806:SF0; PTHR11806:SF0; 1.
DR   Pfam; PF13932; GIDA_assoc; 1.
DR   SUPFAM; SSF51905; SSF51905; 1.
DR   TIGRFAMs; TIGR00136; gidA; 1.
DR   PROSITE; PS01280; GIDA_1; 1.
PE   3: Inferred from homology;
DR   PRODOM; A0LEF5.
DR   SWISS-2DPAGE; A0LEF5.
KW   Complete proteome; Cytoplasm; FAD; Flavoprotein; NAD;
KW   Reference proteome; tRNA processing.
FT   CHAIN         1    642       tRNA uridine 5-carboxymethylaminomethyl
FT                                modification enzyme MnmG.
FT                                /FTId=PRO_0000345348.
FT   NP_BIND      10     15       FAD. {ECO:0000255|HAMAP-Rule:MF_00129}.
FT   NP_BIND     269    283       NAD. {ECO:0000255|HAMAP-Rule:MF_00129}.
FT   BINDING     122    122       FAD; via amide nitrogen and carbonyl
FT                                oxygen. {ECO:0000255|HAMAP-
FT                                Rule:MF_00129}.
FT   BINDING     177    177       FAD. {ECO:0000255|HAMAP-Rule:MF_00129}.
FT   BINDING     366    366       FAD. {ECO:0000255|HAMAP-Rule:MF_00129}.
SQ   SEQUENCE   642 AA;  70555 MW;  331E23E5CC423640 CRC64;
     MNKYEVIVVG AGHAGCEAAL AAARMGCRTL VLSICLDTVA HMPCSPSVGG VGKGHLVREI
     DALGGRMALI TDRTAIQFRL LNTKKGPAVW GTRTQNDKAR YRISMKHCLE SEPNLDLKQA
     HVESLAVEGN RIVGVIDQLG VFFGADAVVL ATGTFLRGLV HIGTTSVEAG RAGELASYPL
     ANQLQRLGFT LGRMKTGTPA RLGRRSIDFS RFREQHGDEA PKPFSLFTDS IALPQVSCFI
     GKTHQRTHEL VRRHIHLSPL YNGTIRGVSA RYCPSLEDKV MRFPDKDFHQ IILEPEGLDT
     EEVYASGTGN SLPYDIQLRL IHSVPGLEEA EVMRPAYAIE YDFVQPTQLK ATLGSKLVEG
     LYMAGQINGT SGYEEAAGQG LWAGINAALK AQGRPPFILD RSEAYLAVMV DDLVTRGTNE
     PYRIFTSRAE YRLLLREDNA DLRLLEKGCE LGLHSADAAK ELRERRGAIR NELERLRLTH
     VRPSAQVNQT LAENHSPPLD APAPLDKLLK RPELNYSTVA ILSPPPEPLS SKVTEQVEVE
     CKYEGYLKRQ EAEVAKFRQL EQAAIPEDLM YDDIPGLSNE LRQKLGAVRP LSLGQATRIP
     GMTPAAVSVL MVHLRRRAQG GPFLAPVLDP GVNMHSQKKK LA
//

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