(data stored in ACNUC7421 zone)

SWISSPROT: LPXK_SYNFM

ID   LPXK_SYNFM              Reviewed;         381 AA.
AC   A0LF44;
DT   10-JUN-2008, integrated into UniProtKB/Swiss-Prot.
DT   12-DEC-2006, sequence version 1.
DT   08-MAY-2019, entry version 68.
DE   RecName: Full=Tetraacyldisaccharide 4'-kinase {ECO:0000255|HAMAP-Rule:MF_00409};
DE            EC=2.7.1.130 {ECO:0000255|HAMAP-Rule:MF_00409};
DE   AltName: Full=Lipid A 4'-kinase {ECO:0000255|HAMAP-Rule:MF_00409};
GN   Name=lpxK {ECO:0000255|HAMAP-Rule:MF_00409};
GN   OrderedLocusNames=Sfum_0346;
OS   Syntrophobacter fumaroxidans (strain DSM 10017 / MPOB).
OC   Bacteria; Proteobacteria; Deltaproteobacteria; Syntrophobacterales;
OC   Syntrophobacteraceae; Syntrophobacter.
OX   NCBI_TaxID=335543;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 10017 / MPOB;
RG   US DOE Joint Genome Institute;
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA   Glavina del Rio T., Hammon N., Israni S., Pitluck S., Goltsman E.G.,
RA   Martinez M., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N.,
RA   Kim E., Boone D.R., Brockman F., Culley D., Ferry J., Gunsalus R.,
RA   McInerney M.J., Morrison M., Plugge C., Rohlin L., Scholten J.,
RA   Sieber J., Stams A.J.M., Worm P., Henstra A.M., Richardson P.;
RT   "Complete sequence of Syntrophobacter fumaroxidans MPOB.";
RL   Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Transfers the gamma-phosphate of ATP to the 4'-position
CC       of a tetraacyldisaccharide 1-phosphate intermediate (termed DS-1-
CC       P) to form tetraacyldisaccharide 1,4'-bis-phosphate (lipid IVA).
CC       {ECO:0000255|HAMAP-Rule:MF_00409}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + (2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-beta-D-
CC         glucosaminyl)-(1->6)-(2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-
CC         alpha-D-glucosaminyl phosphate) = ADP + (2-N,3-O-bis((3R)-3-
CC         hydroxytetradecanoyl)-4-O-phospho-beta-D-glucosaminyl)-(1->6)-
CC         (2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-alpha-D-glucosaminyl
CC         phosphate).; EC=2.7.1.130; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_00409};
CC   -!- PATHWAY: Glycolipid biosynthesis; lipid IV(A) biosynthesis; lipid
CC       IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and
CC       UDP-N-acetyl-alpha-D-glucosamine: step 6/6. {ECO:0000255|HAMAP-
CC       Rule:MF_00409}.
CC   -!- SIMILARITY: Belongs to the LpxK family. {ECO:0000255|HAMAP-
CC       Rule:MF_00409}.
DR   EMBL; CP000478; ABK16046.1; -; Genomic_DNA.
DR   RefSeq; WP_011697219.1; NC_008554.1.
DR   STRING; 335543.Sfum_0346; -.
DR   EnsemblBacteria; ABK16046; ABK16046; Sfum_0346.
DR   KEGG; sfu:Sfum_0346; -.
DR   eggNOG; ENOG4105CHD; Bacteria.
DR   eggNOG; COG1663; LUCA.
DR   HOGENOM; HOG000004954; -.
DR   KO; K00912; -.
DR   OMA; LHPLGNG; -.
DR   OrthoDB; 1382484at2; -.
DR   BioCyc; SFUM335543:G1G7I-351-MONOMER; -.
DR   UniPathway; UPA00359; UER00482.
DR   Proteomes; UP000001784; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0009029; F:tetraacyldisaccharide 4'-kinase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0009245; P:lipid A biosynthetic process; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00409; LpxK; 1.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR003758; Tetraacyldisaccharide_4-kinase.
DR   PANTHER; PTHR42724; PTHR42724; 1.
DR   Pfam; PF02606; LpxK; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR00682; lpxK; 1.
PE   3: Inferred from homology;
DR   PRODOM; A0LF44.
DR   SWISS-2DPAGE; A0LF44.
KW   ATP-binding; Complete proteome; Kinase; Lipid A biosynthesis;
KW   Lipid biosynthesis; Lipid metabolism; Nucleotide-binding;
KW   Reference proteome; Transferase.
FT   CHAIN         1    381       Tetraacyldisaccharide 4'-kinase.
FT                                /FTId=PRO_0000340865.
FT   NP_BIND      78     85       ATP. {ECO:0000255|HAMAP-Rule:MF_00409}.
SQ   SEQUENCE   381 AA;  42058 MW;  C65B3D47123BDDDA CRC64;
     MQTSIILGTG RFQAIRRLSA RVRRLWYSAP ETLRGGPLLS AIKLTSMTYR AGFEMDRRRS
     RFRRRRLPAY VVSVGNLAVG GTGKTPLTLW LARYFKNGGR RVAVLSRGYG RSGSAPGRVP
     SSGELSVLAL EYGDEPAMLA LELGDTPVYV GKHRWESGIL AIESSRADLV ILDDGFQHHA
     LERDLDLVLL DASNPFGNGF TLPLGPLREP KAHLARAHAI VLTRAVEPES VARTRAQLDK
     AFPDKPVFAA QHILRGFHAG LGGAVVPLRS MVARPAVAFA GIADPKSFFS LLEALEIDLR
     MAFAFPDHHR PTARDTAALF DAVRACSADL LITTQKDAVR LPGFLRRVVC VPDLEIDFGE
     DETRFRRFLD RETRGRQAMP E
//

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