(data stored in ACNUC7421 zone)

SWISSPROT: ILVD_SYNFM

ID   ILVD_SYNFM              Reviewed;         557 AA.
AC   A0LF54;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   12-DEC-2006, sequence version 1.
DT   11-DEC-2019, entry version 76.
DE   RecName: Full=Dihydroxy-acid dehydratase {ECO:0000255|HAMAP-Rule:MF_00012};
DE            Short=DAD {ECO:0000255|HAMAP-Rule:MF_00012};
DE            EC=4.2.1.9 {ECO:0000255|HAMAP-Rule:MF_00012};
GN   Name=ilvD {ECO:0000255|HAMAP-Rule:MF_00012}; OrderedLocusNames=Sfum_0356;
OS   Syntrophobacter fumaroxidans (strain DSM 10017 / MPOB).
OC   Bacteria; Proteobacteria; Deltaproteobacteria; Syntrophobacterales;
OC   Syntrophobacteraceae; Syntrophobacter.
OX   NCBI_TaxID=335543;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 10017 / MPOB;
RG   US DOE Joint Genome Institute;
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA   Glavina del Rio T., Hammon N., Israni S., Pitluck S., Goltsman E.G.,
RA   Martinez M., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N.,
RA   Kim E., Boone D.R., Brockman F., Culley D., Ferry J., Gunsalus R.,
RA   McInerney M.J., Morrison M., Plugge C., Rohlin L., Scholten J., Sieber J.,
RA   Stams A.J.M., Worm P., Henstra A.M., Richardson P.;
RT   "Complete sequence of Syntrophobacter fumaroxidans MPOB.";
RL   Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate +
CC         H2O; Xref=Rhea:RHEA:20936, ChEBI:CHEBI:11424, ChEBI:CHEBI:11851,
CC         ChEBI:CHEBI:15377; EC=4.2.1.9; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_00012};
CC   -!- COFACTOR:
CC       Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00012};
CC       Note=Binds 1 [4Fe-4S] cluster. {ECO:0000255|HAMAP-Rule:MF_00012};
CC   -!- PATHWAY: Amino-acid biosynthesis; L-isoleucine biosynthesis; L-
CC       isoleucine from 2-oxobutanoate: step 3/4. {ECO:0000255|HAMAP-
CC       Rule:MF_00012}.
CC   -!- PATHWAY: Amino-acid biosynthesis; L-valine biosynthesis; L-valine from
CC       pyruvate: step 3/4. {ECO:0000255|HAMAP-Rule:MF_00012}.
CC   -!- SIMILARITY: Belongs to the IlvD/Edd family. {ECO:0000255|HAMAP-
CC       Rule:MF_00012}.
DR   EMBL; CP000478; ABK16056.1; -; Genomic_DNA.
DR   RefSeq; WP_011697229.1; NC_008554.1.
DR   SMR; A0LF54; -.
DR   STRING; 335543.Sfum_0356; -.
DR   PRIDE; A0LF54; -.
DR   EnsemblBacteria; ABK16056; ABK16056; Sfum_0356.
DR   KEGG; sfu:Sfum_0356; -.
DR   eggNOG; ENOG4105C01; Bacteria.
DR   eggNOG; COG0129; LUCA.
DR   HOGENOM; HOG000173156; -.
DR   KO; K01687; -.
DR   OMA; IPGHVHL; -.
DR   OrthoDB; 193579at2; -.
DR   BioCyc; SFUM335543:G1G7I-361-MONOMER; -.
DR   UniPathway; UPA00047; UER00057.
DR   UniPathway; UPA00049; UER00061.
DR   Proteomes; UP000001784; Chromosome.
DR   GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0004160; F:dihydroxy-acid dehydratase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0009097; P:isoleucine biosynthetic process; IEA:UniProtKB-UniRule.
DR   GO; GO:0009099; P:valine biosynthetic process; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.50.30.80; -; 1.
DR   HAMAP; MF_00012; IlvD; 1.
DR   InterPro; IPR042096; Dihydro-acid_dehy_C.
DR   InterPro; IPR004404; DihydroxyA_deHydtase.
DR   InterPro; IPR000581; DiOHA_6PGluconate_deHydtase.
DR   InterPro; IPR020558; DiOHA_6PGluconate_deHydtase_CS.
DR   InterPro; IPR037237; IlvD/EDD_N.
DR   Pfam; PF00920; ILVD_EDD; 1.
DR   SUPFAM; SSF143975; SSF143975; 1.
DR   TIGRFAMs; TIGR00110; ilvD; 1.
DR   PROSITE; PS00886; ILVD_EDD_1; 1.
DR   PROSITE; PS00887; ILVD_EDD_2; 1.
PE   3: Inferred from homology;
DR   PRODOM; A0LF54.
DR   SWISS-2DPAGE; A0LF54.
KW   4Fe-4S; Amino-acid biosynthesis; Branched-chain amino acid biosynthesis;
KW   Iron; Iron-sulfur; Lyase; Metal-binding; Reference proteome.
FT   CHAIN           1..557
FT                   /note="Dihydroxy-acid dehydratase"
FT                   /id="PRO_1000001074"
FT   METAL           119
FT                   /note="Iron-sulfur (4Fe-4S)"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00012"
FT   METAL           191
FT                   /note="Iron-sulfur (4Fe-4S)"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00012"
SQ   SEQUENCE   557 AA;  58850 MW;  01B34DC09D6B679F CRC64;
     MRSDLMKKGV EKAPHRSLFK AMGYTQTELD RPLIGVANSA NEIIPGHIHL DKVAEAVKAG
     IRLAGGTPIE FSTIGVCDGI AMNHAGMRYS LASRELIADS VEIMSMAHPF DGLVAVPNCD
     KIIPGMLMAM LRLDIPAILI SGGPMLAGRA GHKQVDLVSV FEAVGSFKAG TITMEELEQL
     EDYACPGCGS CAGMFTANSM NCLSEALGLA LPGNGTIPAV TGARMRLAKL AGMRIVELIK
     ADIRPRQIAT QKAFENAITV DMALGCSTNT VLHVPAIARE AGIDLDLGLF NTLSSRTPHL
     CSLRPGGPHF LENLDQAGGV QAVMKELLAR DLIHRDAMTV TGLTVGKNLE SSKNTDPTVI
     RPISDPYHNE GGIAILYGNL APEGAVVKQS AVAPEMLQRT CRARVFESET DAAGGILDGK
     IKPGDVVVIR YEGPKGGPGM QEMLTPTAAI IGMGLGKDVA LITDGRFSGG TQGAAIGHVS
     PEAAVGGPIA LIEEGDEILV DILNKRLELM VDDATLKARR DKWKAPAPRM TQGYLARYAH
     MVTSGSTGAV LRTEQDK
//

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