(data stored in ACNUC7421 zone)

SWISSPROT: RSMJ_AERHH

ID   RSMJ_AERHH              Reviewed;         248 AA.
AC   A0KEG6;
DT   26-JUN-2007, integrated into UniProtKB/Swiss-Prot.
DT   12-DEC-2006, sequence version 1.
DT   11-DEC-2019, entry version 72.
DE   RecName: Full=Ribosomal RNA small subunit methyltransferase J {ECO:0000255|HAMAP-Rule:MF_01523};
DE            EC=2.1.1.242 {ECO:0000255|HAMAP-Rule:MF_01523};
DE   AltName: Full=16S rRNA m2G1516 methyltransferase {ECO:0000255|HAMAP-Rule:MF_01523};
DE   AltName: Full=rRNA (guanine-N(2)-)-methyltransferase {ECO:0000255|HAMAP-Rule:MF_01523};
GN   Name=rsmJ {ECO:0000255|HAMAP-Rule:MF_01523}; OrderedLocusNames=AHA_0094;
OS   Aeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / DSM 30187 / JCM
OS   1027 / KCTC 2358 / NCIMB 9240).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Aeromonadales;
OC   Aeromonadaceae; Aeromonas.
OX   NCBI_TaxID=380703;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 7966 / DSM 30187 / JCM 1027 / KCTC 2358 / NCIMB 9240;
RX   PubMed=16980456; DOI=10.1128/jb.00621-06;
RA   Seshadri R., Joseph S.W., Chopra A.K., Sha J., Shaw J., Graf J., Haft D.H.,
RA   Wu M., Ren Q., Rosovitz M.J., Madupu R., Tallon L., Kim M., Jin S.,
RA   Vuong H., Stine O.C., Ali A., Horneman A.J., Heidelberg J.F.;
RT   "Genome sequence of Aeromonas hydrophila ATCC 7966T: jack of all trades.";
RL   J. Bacteriol. 188:8272-8282(2006).
CC   -!- FUNCTION: Specifically methylates the guanosine in position 1516 of 16S
CC       rRNA. {ECO:0000255|HAMAP-Rule:MF_01523}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=guanosine(1516) in 16S rRNA + S-adenosyl-L-methionine = H(+) +
CC         N(2)-methylguanosine(1516) in 16S rRNA + S-adenosyl-L-homocysteine;
CC         Xref=Rhea:RHEA:43220, Rhea:RHEA-COMP:10412, Rhea:RHEA-COMP:10413,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC         ChEBI:CHEBI:74269, ChEBI:CHEBI:74481; EC=2.1.1.242;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01523};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01523}.
CC   -!- SIMILARITY: Belongs to the methyltransferase superfamily. RsmJ family.
CC       {ECO:0000255|HAMAP-Rule:MF_01523}.
DR   EMBL; CP000462; ABK36028.1; -; Genomic_DNA.
DR   RefSeq; WP_011704126.1; NC_008570.1.
DR   RefSeq; YP_854618.1; NC_008570.1.
DR   SMR; A0KEG6; -.
DR   STRING; 380703.AHA_0094; -.
DR   PRIDE; A0KEG6; -.
DR   EnsemblBacteria; ABK36028; ABK36028; AHA_0094.
DR   GeneID; 4490989; -.
DR   KEGG; aha:AHA_0094; -.
DR   PATRIC; fig|380703.7.peg.85; -.
DR   eggNOG; ENOG4105FFN; Bacteria.
DR   eggNOG; ENOG410XPRJ; LUCA.
DR   HOGENOM; HOG000218236; -.
DR   KO; K15984; -.
DR   OMA; SRYDIYP; -.
DR   BioCyc; AHYD380703:G1G7B-94-MONOMER; -.
DR   Proteomes; UP000000756; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0008990; F:rRNA (guanine-N2-)-methyltransferase activity; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_01523; 16SrRNA_methyltr_J; 1.
DR   InterPro; IPR007536; 16SrRNA_methylTrfase_J.
DR   InterPro; IPR029063; SAM-dependent_MTases.
DR   PANTHER; PTHR36112; PTHR36112; 1.
DR   Pfam; PF04445; SAM_MT; 1.
DR   SUPFAM; SSF53335; SSF53335; 1.
PE   3: Inferred from homology;
DR   PRODOM; A0KEG6.
DR   SWISS-2DPAGE; A0KEG6.
KW   Cytoplasm; Methyltransferase; Reference proteome; rRNA processing;
KW   S-adenosyl-L-methionine; Transferase.
FT   CHAIN           1..248
FT                   /note="Ribosomal RNA small subunit methyltransferase J"
FT                   /id="PRO_0000292623"
FT   REGION          97..98
FT                   /note="S-adenosyl-L-methionine binding"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01523"
FT   REGION          113..114
FT                   /note="S-adenosyl-L-methionine binding"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01523"
FT   BINDING         167
FT                   /note="S-adenosyl-L-methionine"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01523"
SQ   SEQUENCE   248 AA;  26799 MW;  9B02B89D012D4463 CRC64;
     MQILLEDPAK APQAEALASQ LGQTDLPQFA LVFTAARLEL RKLDEPKLGA VYVDFVEGAV
     AHRRKFGGGR GQSIAKAVGL KAGAMPRVVD ATAGLGRDAF VLASLGCKVT MIERSPVVAA
     LLQDGLARAA LDPEIGPWVR ERMQLRHGPA VETLLGLTER PDVIYLDPMF PHKQKSALVK
     KEMRVFQSLV GPDLDADALL PAALAMADKR VVVKRPDYAG WLNEHKPSMA IETKSNRFDV
     YVMAALAG
//

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