(data stored in ACNUC7421 zone)

SWISSPROT: A0KER4_AERHH

ID   A0KER4_AERHH            Unreviewed;       917 AA.
AC   A0KER4;
DT   12-DEC-2006, integrated into UniProtKB/TrEMBL.
DT   12-DEC-2006, sequence version 1.
DT   08-MAY-2019, entry version 100.
DE   RecName: Full=DNA polymerase I {ECO:0000256|RuleBase:RU004460};
DE            EC=2.7.7.7 {ECO:0000256|RuleBase:RU004460};
GN   Name=polA {ECO:0000256|RuleBase:RU004460,
GN   ECO:0000313|EMBL:ABK36807.1};
GN   OrderedLocusNames=AHA_0196 {ECO:0000313|EMBL:ABK36807.1};
OS   Aeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / DSM 30187 /
OS   JCM 1027 / KCTC 2358 / NCIMB 9240).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Aeromonadales;
OC   Aeromonadaceae; Aeromonas.
OX   NCBI_TaxID=380703 {ECO:0000313|EMBL:ABK36807.1, ECO:0000313|Proteomes:UP000000756};
RN   [1] {ECO:0000313|EMBL:ABK36807.1, ECO:0000313|Proteomes:UP000000756}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 7966 / DSM 30187 / JCM 1027 / KCTC 2358 / NCIMB 9240
RC   {ECO:0000313|Proteomes:UP000000756};
RX   PubMed=16980456; DOI=10.1128/JB.00621-06;
RA   Seshadri R., Joseph S.W., Chopra A.K., Sha J., Shaw J., Graf J.,
RA   Haft D., Wu M., Ren Q., Rosovitz M.J., Madupu R., Tallon L., Kim M.,
RA   Jin S., Vuong H., Stine O.C., Ali A., Horneman A.J., Heidelberg J.F.;
RT   "Genome sequence of Aeromonas hydrophila ATCC 7966T: jack of all
RT   trades.";
RL   J. Bacteriol. 188:8272-8282(2006).
CC   -!- FUNCTION: In addition to polymerase activity, this DNA polymerase
CC       exhibits 5'-3' exonuclease activity.
CC       {ECO:0000256|RuleBase:RU004460}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC         diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-
CC         COMP:11130, Rhea:RHEA-COMP:11131, ChEBI:CHEBI:33019,
CC         ChEBI:CHEBI:61560, ChEBI:CHEBI:83828; EC=2.7.7.7;
CC         Evidence={ECO:0000256|RuleBase:RU004460};
CC   -!- SIMILARITY: Belongs to the DNA polymerase type-A family.
CC       {ECO:0000256|RuleBase:RU004460}.
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DR   EMBL; CP000462; ABK36807.1; -; Genomic_DNA.
DR   RefSeq; WP_011704214.1; NC_008570.1.
DR   RefSeq; YP_854729.1; NC_008570.1.
DR   STRING; 380703.AHA_0196; -.
DR   EnsemblBacteria; ABK36807; ABK36807; AHA_0196.
DR   GeneID; 4489080; -.
DR   KEGG; aha:AHA_0196; -.
DR   PATRIC; fig|380703.7.peg.189; -.
DR   eggNOG; ENOG4105C2M; Bacteria.
DR   eggNOG; COG0258; LUCA.
DR   eggNOG; COG0749; LUCA.
DR   HOGENOM; HOG000020997; -.
DR   KO; K02335; -.
DR   OMA; ETGRVHT; -.
DR   BioCyc; AHYD380703:G1G7B-196-MONOMER; -.
DR   Proteomes; UP000000756; Chromosome.
DR   GO; GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule.
DR   GO; GO:0006261; P:DNA-dependent DNA replication; IEA:UniProtKB-UniRule.
DR   CDD; cd09898; H3TH_53EXO; 1.
DR   Gene3D; 3.30.420.10; -; 1.
DR   InterPro; IPR002562; 3'-5'_exonuclease_dom.
DR   InterPro; IPR020046; 5-3_exonucl_a-hlix_arch_N.
DR   InterPro; IPR036279; 5-3_exonuclease_C_sf.
DR   InterPro; IPR002421; 5-3_exonuclease_N.
DR   InterPro; IPR019760; DNA-dir_DNA_pol_A_CS.
DR   InterPro; IPR001098; DNA-dir_DNA_pol_A_palm_dom.
DR   InterPro; IPR020045; DNA_polI_H3TH.
DR   InterPro; IPR018320; DNA_polymerase_1.
DR   InterPro; IPR002298; DNA_polymerase_A.
DR   InterPro; IPR008918; HhH2.
DR   InterPro; IPR029060; PIN-like_dom_sf.
DR   InterPro; IPR012337; RNaseH-like_sf.
DR   InterPro; IPR036397; RNaseH_sf.
DR   PANTHER; PTHR10133; PTHR10133; 1.
DR   Pfam; PF01367; 5_3_exonuc; 1.
DR   Pfam; PF02739; 5_3_exonuc_N; 1.
DR   Pfam; PF00476; DNA_pol_A; 1.
DR   Pfam; PF01612; DNA_pol_A_exo1; 1.
DR   PRINTS; PR00868; DNAPOLI.
DR   SMART; SM00474; 35EXOc; 1.
DR   SMART; SM00475; 53EXOc; 1.
DR   SMART; SM00279; HhH2; 1.
DR   SMART; SM00482; POLAc; 1.
DR   SUPFAM; SSF47807; SSF47807; 1.
DR   SUPFAM; SSF53098; SSF53098; 1.
DR   SUPFAM; SSF88723; SSF88723; 1.
DR   TIGRFAMs; TIGR00593; pola; 1.
DR   PROSITE; PS00447; DNA_POLYMERASE_A; 1.
PE   3: Inferred from homology;
DR   PRODOM; A0KER4.
DR   SWISS-2DPAGE; A0KER4.
KW   Coiled coil {ECO:0000256|SAM:Coils};
KW   Complete proteome {ECO:0000313|Proteomes:UP000000756};
KW   DNA damage {ECO:0000256|RuleBase:RU004460};
KW   DNA repair {ECO:0000256|RuleBase:RU004460};
KW   DNA replication {ECO:0000256|RuleBase:RU004460};
KW   DNA-binding {ECO:0000256|RuleBase:RU004460,
KW   ECO:0000256|SAAS:SAAS00882656};
KW   DNA-directed DNA polymerase {ECO:0000256|RuleBase:RU004460};
KW   Hydrolase {ECO:0000256|SAAS:SAAS00427537};
KW   Nuclease {ECO:0000256|SAAS:SAAS00427491};
KW   Nucleotidyltransferase {ECO:0000256|RuleBase:RU004460,
KW   ECO:0000313|EMBL:ABK36807.1};
KW   Reference proteome {ECO:0000313|Proteomes:UP000000756};
KW   Transferase {ECO:0000256|RuleBase:RU004460,
KW   ECO:0000313|EMBL:ABK36807.1}.
FT   DOMAIN        5    268       53EXOc. {ECO:0000259|SMART:SM00475}.
FT   DOMAIN      318    505       3'-5' exonuclease. {ECO:0000259|SMART:
FT                                SM00474}.
FT   DOMAIN      674    881       POLAc. {ECO:0000259|SMART:SM00482}.
FT   COILED      544    564       {ECO:0000256|SAM:Coils}.
SQ   SEQUENCE   917 AA;  101324 MW;  C0F356CEDB455D45 CRC64;
     MAPSNPLILV DGSSYLYRAF FASQQADLRT STGLPSGAVR VMANMMRSLR KQYPDCHVAV
     VFDAKGKTFR DDIYPEYKAN RASMPDDLRS QVAPIHQMIK AMGFPFLMVE GVEADDVIGT
     LARQATEKQL PVLISTGDKD MAQLVSDHVT LIDTMKDVKT DREGVIEKFG VPPELIIDYL
     ALMGDKVDNI PGMTGVGEKT ALALLQGIGS IDEIAANLDK VAALGFRGSK AFADKFREQE
     EQVRLSYVLA TIKTDVALEQ SLEELQLGPI DKEALLAVYR EYELRNLIKE LESGGAEESG
     AEGDDEGAAP VAAIETDYRC ILDEAEFDEW LARLQAAPLF AFDTETTSLD YMEARVVGVS
     FAIEPGKAAY VPFGHDYLGA PVQLTEAVVL GKLKPLLEDP ARLKVGQNLK YDRNVLLNHG
     IELQGIAYDT MLESYVLNST ASRHDMDSLA RRYLNAETIS FEEIAGKGVK QLTFNQIELE
     QAAPYAAEDA DITLRLHQAL WGKLSAEPGL AKVFSEIELP LLPVLARMER LGTTIEPKLL
     HQQSQEIEVR LAELEKQAHE LAGQEFNLSS PKQLGEILFT KLGLPIIKKT PKGAPSTAEE
     VLAELAETYE LPQLLMEHRG LAKLKSTYTD KLPLMIKPQT GRVHTSYHQA VAATGRLSST
     DPNLQNIPVR NEQGRRIRQA FIPCAGYKLV AADYSQIELR IMAHLSGDKG LLTAFAEGKD
     IHKATAAEVF GVALEAVTSD MRRSAKAINF GLIYGMSAFG LAKQLGIGRA EAQKYMDLYF
     ERYPGVLEYM ERTRQQADAQ GYVETLFGRR LYLPDIKSRN AGLRKAAERA AINAPMQGTA
     ADIIKRAMIN VDGWIRGIED QSIRMLMQVH DELVFEIREE KLEEYIAIIK EKMSAAAELD
     VPLVVEAGTG DNWDQAH
//

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