(data stored in ACNUC7421 zone)

SWISSPROT: A0KEN8_AERHH

ID   A0KEN8_AERHH            Unreviewed;       421 AA.
AC   A0KEN8;
DT   12-DEC-2006, integrated into UniProtKB/TrEMBL.
DT   12-DEC-2006, sequence version 1.
DT   08-MAY-2019, entry version 64.
DE   RecName: Full=3-deoxy-D-manno-octulosonic acid transferase {ECO:0000256|RuleBase:RU365103};
DE            Short=Kdo transferase {ECO:0000256|RuleBase:RU365103};
DE            EC=2.4.99.12 {ECO:0000256|RuleBase:RU365103};
DE   AltName: Full=Lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase {ECO:0000256|RuleBase:RU365103};
GN   Name=waaA {ECO:0000313|EMBL:ABK38792.1};
GN   OrderedLocusNames=AHA_0170 {ECO:0000313|EMBL:ABK38792.1};
OS   Aeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / DSM 30187 /
OS   JCM 1027 / KCTC 2358 / NCIMB 9240).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Aeromonadales;
OC   Aeromonadaceae; Aeromonas.
OX   NCBI_TaxID=380703 {ECO:0000313|EMBL:ABK38792.1, ECO:0000313|Proteomes:UP000000756};
RN   [1] {ECO:0000313|EMBL:ABK38792.1, ECO:0000313|Proteomes:UP000000756}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 7966 / DSM 30187 / JCM 1027 / KCTC 2358 / NCIMB 9240
RC   {ECO:0000313|Proteomes:UP000000756};
RX   PubMed=16980456; DOI=10.1128/JB.00621-06;
RA   Seshadri R., Joseph S.W., Chopra A.K., Sha J., Shaw J., Graf J.,
RA   Haft D., Wu M., Ren Q., Rosovitz M.J., Madupu R., Tallon L., Kim M.,
RA   Jin S., Vuong H., Stine O.C., Ali A., Horneman A.J., Heidelberg J.F.;
RT   "Genome sequence of Aeromonas hydrophila ATCC 7966T: jack of all
RT   trades.";
RL   J. Bacteriol. 188:8272-8282(2006).
CC   -!- FUNCTION: Involved in lipopolysaccharide (LPS) biosynthesis.
CC       Catalyzes the transfer of 3-deoxy-D-manno-octulosonate (Kdo)
CC       residue(s) from CMP-Kdo to lipid IV(A), the tetraacyldisaccharide-
CC       1,4'-bisphosphate precursor of lipid A.
CC       {ECO:0000256|RuleBase:RU365103}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=CMP-3-deoxy-beta-D-manno-octulosonate + lipid IVA (E.
CC         coli) = alpha-Kdo-(2->6)-lipid IVA + CMP + H(+);
CC         Xref=Rhea:RHEA:28066, ChEBI:CHEBI:15378, ChEBI:CHEBI:58603,
CC         ChEBI:CHEBI:60364, ChEBI:CHEBI:60377, ChEBI:CHEBI:85987;
CC         EC=2.4.99.12; Evidence={ECO:0000256|RuleBase:RU365103};
CC   -!- PATHWAY: Bacterial outer membrane biogenesis; LPS core
CC       biosynthesis. {ECO:0000256|RuleBase:RU365103}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane
CC       {ECO:0000256|RuleBase:RU365103}.
CC   -!- SIMILARITY: Belongs to the glycosyltransferase group 1 family.
CC       {ECO:0000256|RuleBase:RU365103}.
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DR   EMBL; CP000462; ABK38792.1; -; Genomic_DNA.
DR   RefSeq; WP_011704189.1; NC_008570.1.
DR   RefSeq; YP_854703.1; NC_008570.1.
DR   STRING; 380703.AHA_0170; -.
DR   CAZy; GT30; Glycosyltransferase Family 30.
DR   EnsemblBacteria; ABK38792; ABK38792; AHA_0170.
DR   GeneID; 4487433; -.
DR   KEGG; aha:AHA_0170; -.
DR   PATRIC; fig|380703.7.peg.161; -.
DR   eggNOG; ENOG4105D8A; Bacteria.
DR   eggNOG; COG1519; LUCA.
DR   HOGENOM; HOG000257156; -.
DR   KO; K02527; -.
DR   OMA; FIKYEFW; -.
DR   BioCyc; AHYD380703:G1G7B-170-MONOMER; -.
DR   UniPathway; UPA00958; -.
DR   Proteomes; UP000000756; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-UniRule.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016740; F:transferase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0009244; P:lipopolysaccharide core region biosynthetic process; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.40.50.11720; -; 1.
DR   InterPro; IPR001296; Glyco_trans_1.
DR   InterPro; IPR007507; Glycos_transf_N.
DR   InterPro; IPR038107; Glycos_transf_N_sf.
DR   InterPro; IPR039901; Kdotransferase.
DR   PANTHER; PTHR42755; PTHR42755; 1.
DR   Pfam; PF00534; Glycos_transf_1; 1.
DR   Pfam; PF04413; Glycos_transf_N; 1.
PE   3: Inferred from homology;
DR   PRODOM; A0KEN8.
DR   SWISS-2DPAGE; A0KEN8.
KW   Cell inner membrane {ECO:0000256|RuleBase:RU365103};
KW   Cell membrane {ECO:0000256|RuleBase:RU365103};
KW   Complete proteome {ECO:0000313|Proteomes:UP000000756};
KW   Lipopolysaccharide biosynthesis {ECO:0000256|RuleBase:RU365103};
KW   Membrane {ECO:0000256|RuleBase:RU365103};
KW   Reference proteome {ECO:0000313|Proteomes:UP000000756};
KW   Transferase {ECO:0000256|RuleBase:RU365103,
KW   ECO:0000313|EMBL:ABK38792.1};
KW   Transmembrane {ECO:0000256|RuleBase:RU365103};
KW   Transmembrane helix {ECO:0000256|RuleBase:RU365103}.
FT   TRANSMEM      6     23       Helical. {ECO:0000256|RuleBase:RU365103}.
FT   DOMAIN       34    211       Glycos_transf_N. {ECO:0000259|Pfam:
FT                                PF04413}.
FT   DOMAIN      300    398       Glycos_transf_1. {ECO:0000259|Pfam:
FT                                PF00534}.
SQ   SEQUENCE   421 AA;  45926 MW;  17FA592EAE5C4642 CRC64;
     MGYRLLYNLL IHLGLPLALL ALYKPKKGKP GFGKRWAEHL GRTPATGQEA PLWIHAVSVG
     ETLAISPFIR ALKAERPDLP ILLTTTTRTG AEQAARLGDL VEHRYAPLDY PWAVAAFLNA
     VKPRALWVME TELWPNWLAA CEERHLPVTI INARLSERSC RRYGRFQGAF DALSQPLTHL
     LCQHQDDADR FHRLGLGRER LAVTGSIKFD IQLDEQVQAR GRALRQLLGA ERPVWIAAST
     HQGEDEQVLA AFDRVLAAQP SALLILVPRH PERFERVAAL CAPYGCVRRT AGGAVGEHDK
     VYLGDTMGEL PLMLAAADVA FVGGSLVKVG GHNLLEPAAL GKPCLSGPAY FNFSDITRQL
     VAQGGAAVVA DAAALGEQVS ALLADESRRR EMGEQARAVV LRNQGALART LSHCLASLEN
     D
//

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