(data stored in ACNUC7421 zone)

SWISSPROT: SSRP_PARDP

ID   SSRP_PARDP              Reviewed;         156 AA.
AC   A1AZQ0;
DT   29-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 1.
DT   08-MAY-2019, entry version 79.
DE   RecName: Full=SsrA-binding protein {ECO:0000255|HAMAP-Rule:MF_00023};
DE   AltName: Full=Small protein B {ECO:0000255|HAMAP-Rule:MF_00023};
GN   Name=smpB {ECO:0000255|HAMAP-Rule:MF_00023};
GN   OrderedLocusNames=Pden_0632;
OS   Paracoccus denitrificans (strain Pd 1222).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales;
OC   Rhodobacteraceae; Paracoccus.
OX   NCBI_TaxID=318586;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Pd 1222;
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA   Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H.,
RA   Pitluck S., Munk A.C., Brettin T., Bruce D., Han C., Tapia R.,
RA   Gilna P., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N.,
RA   Lykidis A., Spiro S., Richardson D.J., Moir J.W.B., Ferguson S.J.,
RA   van Spanning R.J.M., Richardson P.;
RT   "Complete sequence of chromosome 1 of Paracoccus denitrificans
RT   PD1222.";
RL   Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Required for rescue of stalled ribosomes mediated by
CC       trans-translation. Binds to transfer-messenger RNA (tmRNA),
CC       required for stable association of tmRNA with ribosomes. tmRNA and
CC       SmpB together mimic tRNA shape, replacing the anticodon stem-loop
CC       with SmpB. tmRNA is encoded by the ssrA gene; the 2 termini fold
CC       to resemble tRNA(Ala) and it encodes a 'tag peptide', a short
CC       internal open reading frame. During trans-translation Ala-
CC       aminoacylated tmRNA acts like a tRNA, entering the A-site of
CC       stalled ribosomes, displacing the stalled mRNA. The ribosome then
CC       switches to translate the ORF on the tmRNA; the nascent peptide is
CC       terminated with the 'tag peptide' encoded by the tmRNA and
CC       targeted for degradation. The ribosome is freed to recommence
CC       translation, which seems to be the essential function of trans-
CC       translation. {ECO:0000255|HAMAP-Rule:MF_00023}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00023}.
CC       Note=The tmRNA-SmpB complex associates with stalled 70S ribosomes.
CC       {ECO:0000255|HAMAP-Rule:MF_00023}.
CC   -!- SIMILARITY: Belongs to the SmpB family. {ECO:0000255|HAMAP-
CC       Rule:MF_00023}.
DR   EMBL; CP000489; ABL68744.1; -; Genomic_DNA.
DR   RefSeq; WP_011746977.1; NC_008686.1.
DR   SMR; A1AZQ0; -.
DR   STRING; 318586.Pden_0632; -.
DR   PRIDE; A1AZQ0; -.
DR   EnsemblBacteria; ABL68744; ABL68744; Pden_0632.
DR   KEGG; pde:Pden_0632; -.
DR   eggNOG; ENOG4108UH4; Bacteria.
DR   eggNOG; COG0691; LUCA.
DR   HOGENOM; HOG000009628; -.
DR   KO; K03664; -.
DR   OMA; KLHDKRA; -.
DR   BioCyc; PDEN318586:G1GW1-639-MONOMER; -.
DR   Proteomes; UP000000361; Chromosome 1.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0070929; P:trans-translation; IEA:UniProtKB-UniRule.
DR   CDD; cd09294; SmpB; 1.
DR   Gene3D; 2.40.280.10; -; 1.
DR   HAMAP; MF_00023; SmpB; 1.
DR   InterPro; IPR023620; SmpB.
DR   InterPro; IPR000037; SsrA-bd_prot.
DR   PANTHER; PTHR30308; PTHR30308; 1.
DR   Pfam; PF01668; SmpB; 1.
DR   ProDom; PD004488; SmpB; 1.
DR   SUPFAM; SSF74982; SSF74982; 1.
DR   TIGRFAMs; TIGR00086; smpB; 1.
PE   3: Inferred from homology;
DR   PRODOM; A1AZQ0.
DR   SWISS-2DPAGE; A1AZQ0.
KW   Complete proteome; Cytoplasm; Reference proteome; RNA-binding.
FT   CHAIN         1    156       SsrA-binding protein.
FT                                /FTId=PRO_0000331071.
SQ   SEQUENCE   156 AA;  17873 MW;  5491475C0A8FA499 CRC64;
     MATKSDPNYK IIAENRRARF DYFIESDLEV GIVLTGSEVK SLRTGQSNIA ESYASVENGE
     LWLINAYIAA YKQAGVFGHE ERRRRKLLVS RKELARLWQA IGREGMTLVP LVMYFNDRGK
     VKLKIGVAKG KKVADKRETA AKRDWNRQKQ RLLKQG
//

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