(data stored in ACNUC7421 zone)

SWISSPROT: SYGB_POLNA

ID   SYGB_POLNA              Reviewed;         711 AA.
AC   A1VJD8;
DT   24-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT   06-FEB-2007, sequence version 1.
DT   11-DEC-2019, entry version 81.
DE   RecName: Full=Glycine--tRNA ligase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            EC=6.1.1.14 {ECO:0000255|HAMAP-Rule:MF_00255};
DE   AltName: Full=Glycyl-tRNA synthetase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            Short=GlyRS {ECO:0000255|HAMAP-Rule:MF_00255};
GN   Name=glyS {ECO:0000255|HAMAP-Rule:MF_00255}; OrderedLocusNames=Pnap_0444;
OS   Polaromonas naphthalenivorans (strain CJ2).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Comamonadaceae; Polaromonas.
OX   NCBI_TaxID=365044;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CJ2;
RX   PubMed=19453698; DOI=10.1111/j.1462-2920.2009.01947.x;
RA   Yagi J.M., Sims D., Brettin T., Bruce D., Madsen E.L.;
RT   "The genome of Polaromonas naphthalenivorans strain CJ2, isolated from coal
RT   tar-contaminated sediment, reveals physiological and metabolic versatility
RT   and evolution through extensive horizontal gene transfer.";
RL   Environ. Microbiol. 11:2253-2270(2009).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-
CC         tRNA(Gly); Xref=Rhea:RHEA:16013, Rhea:RHEA-COMP:9664, Rhea:RHEA-
CC         COMP:9683, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57305,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78522, ChEBI:CHEBI:456215;
CC         EC=6.1.1.14; Evidence={ECO:0000255|HAMAP-Rule:MF_00255};
CC   -!- SUBUNIT: Tetramer of two alpha and two beta subunits.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
DR   EMBL; CP000529; ABM35766.1; -; Genomic_DNA.
DR   RefSeq; WP_011799866.1; NC_008781.1.
DR   SMR; A1VJD8; -.
DR   STRING; 365044.Pnap_0444; -.
DR   PRIDE; A1VJD8; -.
DR   EnsemblBacteria; ABM35766; ABM35766; Pnap_0444.
DR   KEGG; pna:Pnap_0444; -.
DR   eggNOG; ENOG4105C38; Bacteria.
DR   eggNOG; COG0751; LUCA.
DR   HOGENOM; HOG000264302; -.
DR   KO; K01879; -.
DR   OMA; LPIPKRM; -.
DR   OrthoDB; 213210at2; -.
DR   Proteomes; UP000000644; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004814; F:arginine-tRNA ligase activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004820; F:glycine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006420; P:arginyl-tRNA aminoacylation; IEA:InterPro.
DR   GO; GO:0006426; P:glycyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00255; Gly_tRNA_synth_beta; 1.
DR   InterPro; IPR008909; DALR_anticod-bd.
DR   InterPro; IPR015944; Gly-tRNA-synth_bsu.
DR   InterPro; IPR006194; Gly-tRNA-synth_heterodimer.
DR   PANTHER; PTHR30075; PTHR30075; 1.
DR   Pfam; PF05746; DALR_1; 1.
DR   Pfam; PF02092; tRNA_synt_2f; 1.
DR   PRINTS; PR01045; TRNASYNTHGB.
DR   TIGRFAMs; TIGR00211; glyS; 1.
DR   PROSITE; PS50861; AA_TRNA_LIGASE_II_GLYAB; 1.
PE   3: Inferred from homology;
DR   PRODOM; A1VJD8.
DR   SWISS-2DPAGE; A1VJD8.
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..711
FT                   /note="Glycine--tRNA ligase beta subunit"
FT                   /id="PRO_1000101315"
SQ   SEQUENCE   711 AA;  76087 MW;  8C0CDD4104A35B78 CRC64;
     MTTKNLLVEL FVEELPPKAL KKLGDAFAGV LAEQLKALGL ATAESVVTAY ASPRRLAAHI
     SHVWLKADDK AVQQKLMPVS VGLDSAGHAT PALLKRLQAL GADLSDPVAA VAALKRAPDG
     KAEALFYNSL VTGATLDTGL QKALEEAIAK LPIPKVMSYQ LETDCELPGW TSVNFVRPAH
     GLVALHGSTV VPVKVLGLKA GNSTRGHRFE AAVDPVVLAD ADSYAVTLEI DGAVIASFAQ
     RKAEIARQLA EAAAELGGGV QPIEDDALLD EVTALVERPN VLVCEFEKEF LDVPQECLIL
     TMKANQKYFP LLDAAGKLTN KFLVVSNISP EDASFVIGGN ERVVRPRLAD AKFFFDQDRK
     RTLASRVEGL GKVVYHNKLG TQGERTERVA EIAVKIAQLL EGDALAGKAG KAAWLAKADL
     LTDMVGEFPE LQGTMGRYYA LHDGHSAEIA AAIEDHYKPR FAGDELPRNP VGVVVALADK
     LETLVGMFGI GNLPTGDKDP FALRRHALGV IRMLVEKDLP LDLGALVGGA APVFGDKITD
     ATPALLDFIY DRLSGSLREQ GYSAQEVDSV VSQKPQRLGD VPKRLAAVRA FAALPEAPAL
     AAANKRIGNI LKKEALEVDP HVSELLLREA AEIALYAAMR DVVPTANAQF DTGDYTASLQ
     TLAALRAPVD AFFDGVMVNA EELDLRLNRQ GLLKSLHNAM NRVADLSRLV A
//

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