(data stored in ACNUC7421 zone)

SWISSPROT: DUT_SHESH

ID   DUT_SHESH               Reviewed;         152 AA.
AC   A8FQ73;
DT   20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT   13-NOV-2007, sequence version 1.
DT   07-JUN-2017, entry version 69.
DE   RecName: Full=Deoxyuridine 5'-triphosphate nucleotidohydrolase {ECO:0000255|HAMAP-Rule:MF_00116};
DE            Short=dUTPase {ECO:0000255|HAMAP-Rule:MF_00116};
DE            EC=3.6.1.23 {ECO:0000255|HAMAP-Rule:MF_00116};
DE   AltName: Full=dUTP pyrophosphatase {ECO:0000255|HAMAP-Rule:MF_00116};
GN   Name=dut {ECO:0000255|HAMAP-Rule:MF_00116};
GN   OrderedLocusNames=Ssed_0383;
OS   Shewanella sediminis (strain HAW-EB3).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales;
OC   Shewanellaceae; Shewanella.
OX   NCBI_TaxID=425104;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=HAW-EB3;
RG   US DOE Joint Genome Institute;
RA   Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T.,
RA   Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D.,
RA   Detter J.C., Han C., Schmutz J., Larimer F., Land M., Hauser L.,
RA   Kyrpides N., Kim E., Zhao J.-S., Richardson P.;
RT   "Complete sequence of Shewanella sediminis HAW-EB3.";
RL   Submitted (AUG-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: This enzyme is involved in nucleotide metabolism: it
CC       produces dUMP, the immediate precursor of thymidine nucleotides
CC       and it decreases the intracellular concentration of dUTP so that
CC       uracil cannot be incorporated into DNA. {ECO:0000255|HAMAP-
CC       Rule:MF_00116}.
CC   -!- CATALYTIC ACTIVITY: dUTP + H(2)O = dUMP + diphosphate.
CC       {ECO:0000255|HAMAP-Rule:MF_00116}.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00116};
CC   -!- PATHWAY: Pyrimidine metabolism; dUMP biosynthesis; dUMP from dCTP
CC       (dUTP route): step 2/2. {ECO:0000255|HAMAP-Rule:MF_00116}.
CC   -!- SIMILARITY: Belongs to the dUTPase family. {ECO:0000255|HAMAP-
CC       Rule:MF_00116}.
DR   EMBL; CP000821; ABV34996.1; -; Genomic_DNA.
DR   RefSeq; WP_012140734.1; NC_009831.1.
DR   ProteinModelPortal; A8FQ73; -.
DR   SMR; A8FQ73; -.
DR   STRING; 425104.Ssed_0383; -.
DR   EnsemblBacteria; ABV34996; ABV34996; Ssed_0383.
DR   KEGG; sse:Ssed_0383; -.
DR   eggNOG; ENOG4108Z1K; Bacteria.
DR   eggNOG; COG0756; LUCA.
DR   HOGENOM; HOG000028968; -.
DR   KO; K01520; -.
DR   OMA; GVILINH; -.
DR   OrthoDB; POG091H01I6; -.
DR   UniPathway; UPA00610; UER00666.
DR   Proteomes; UP000002015; Chromosome.
DR   GO; GO:0004170; F:dUTP diphosphatase activity; IEA:UniProtKB-EC.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0006226; P:dUMP biosynthetic process; IEA:UniProtKB-UniPathway.
DR   GO; GO:0046080; P:dUTP metabolic process; IEA:InterPro.
DR   CDD; cd07557; trimeric_dUTPase; 1.
DR   HAMAP; MF_00116; dUTPase_bact; 1.
DR   InterPro; IPR029054; dUTPase-like.
DR   InterPro; IPR008181; dUTPase_1.
DR   InterPro; IPR033704; dUTPase_trimeric.
DR   Pfam; PF00692; dUTPase; 1.
DR   SUPFAM; SSF51283; SSF51283; 1.
DR   TIGRFAMs; TIGR00576; dut; 1.
PE   3: Inferred from homology;
DR   PRODOM; A8FQ73.
DR   SWISS-2DPAGE; A8FQ73.
KW   Complete proteome; Hydrolase; Magnesium; Metal-binding;
KW   Nucleotide metabolism.
FT   CHAIN         1    152       Deoxyuridine 5'-triphosphate
FT                                nucleotidohydrolase.
FT                                /FTId=PRO_1000076073.
FT   REGION       71     73       Substrate binding. {ECO:0000255|HAMAP-
FT                                Rule:MF_00116}.
FT   REGION       88     90       Substrate binding. {ECO:0000255|HAMAP-
FT                                Rule:MF_00116}.
FT   BINDING      84     84       Substrate. {ECO:0000255|HAMAP-
FT                                Rule:MF_00116}.
FT   BINDING      98     98       Substrate; via amide nitrogen and
FT                                carbonyl oxygen. {ECO:0000255|HAMAP-
FT                                Rule:MF_00116}.
SQ   SEQUENCE   152 AA;  16098 MW;  BAFF604292974BCF CRC64;
     MKTPIEVKIL DSRIGSQFPL PAYATPGSAG MDLRAMLETS IQVAPGETIL IPTGISIHVA
     DSSLAAVILP RSGLGHKHGI VLGNLVGLID SDYQGPLMVS CWNRGSEPFT LEIGDRLAQL
     VFVPVVQAEF KLVDEFDNSS RGEGGFGHSG TK
//

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