(data stored in ACNUC7421 zone)

SWISSPROT: KEFF_ECODH

ID   KEFF_ECODH              Reviewed;         176 AA.
AC   B1XC47;
DT   14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT   20-MAY-2008, sequence version 1.
DT   05-JUL-2017, entry version 55.
DE   RecName: Full=Glutathione-regulated potassium-efflux system ancillary protein KefF {ECO:0000255|HAMAP-Rule:MF_01414};
DE   AltName: Full=Quinone oxidoreductase KefF {ECO:0000255|HAMAP-Rule:MF_01414};
DE            EC=1.6.5.2 {ECO:0000255|HAMAP-Rule:MF_01414};
GN   Name=kefF {ECO:0000255|HAMAP-Rule:MF_01414};
GN   OrderedLocusNames=ECDH10B_0047;
OS   Escherichia coli (strain K12 / DH10B).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=316385;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / DH10B;
RX   PubMed=18245285; DOI=10.1128/JB.01695-07;
RA   Durfee T., Nelson R., Baldwin S., Plunkett G. III, Burland V., Mau B.,
RA   Petrosino J.F., Qin X., Muzny D.M., Ayele M., Gibbs R.A., Csorgo B.,
RA   Posfai G., Weinstock G.M., Blattner F.R.;
RT   "The complete genome sequence of Escherichia coli DH10B: insights into
RT   the biology of a laboratory workhorse.";
RL   J. Bacteriol. 190:2597-2606(2008).
CC   -!- FUNCTION: Regulatory subunit of a potassium efflux system that
CC       confers protection against electrophiles. Required for full
CC       activity of KefC. Shows redox enzymatic activity, but this
CC       enzymatic activity is not required for activation of KefC.
CC       {ECO:0000255|HAMAP-Rule:MF_01414}.
CC   -!- CATALYTIC ACTIVITY: NAD(P)H + a quinone = NAD(P)(+) + a
CC       hydroquinone. {ECO:0000255|HAMAP-Rule:MF_01414}.
CC   -!- COFACTOR:
CC       Name=FMN; Xref=ChEBI:CHEBI:58210;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01414};
CC   -!- SUBUNIT: Homodimer. Interacts with KefC. {ECO:0000255|HAMAP-
CC       Rule:MF_01414}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC       Rule:MF_01414}; Peripheral membrane protein {ECO:0000255|HAMAP-
CC       Rule:MF_01414}; Cytoplasmic side {ECO:0000255|HAMAP-
CC       Rule:MF_01414}.
CC   -!- SIMILARITY: Belongs to the NAD(P)H dehydrogenase (quinone) family.
CC       KefF subfamily. {ECO:0000255|HAMAP-Rule:MF_01414}.
DR   EMBL; CP000948; ACB01251.1; -; Genomic_DNA.
DR   RefSeq; WP_000600725.1; NC_010473.1.
DR   ProteinModelPortal; B1XC47; -.
DR   SMR; B1XC47; -.
DR   EnsemblBacteria; ACB01251; ACB01251; ECDH10B_0047.
DR   KEGG; ecd:ECDH10B_0047; -.
DR   eggNOG; ENOG4108MXQ; Bacteria.
DR   eggNOG; COG2249; LUCA.
DR   HOGENOM; HOG000149972; -.
DR   KO; K11746; -.
DR   OMA; YWYSTPS; -.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0003955; F:NAD(P)H dehydrogenase (quinone) activity; IEA:UniProtKB-EC.
DR   GO; GO:0006813; P:potassium ion transport; IEA:InterPro.
DR   Gene3D; 3.40.50.360; -; 1.
DR   HAMAP; MF_01414; K_H_efflux_KefF; 1.
DR   InterPro; IPR003680; Flavodoxin_fold.
DR   InterPro; IPR029039; Flavoprotein-like_dom.
DR   InterPro; IPR023948; K_H_efflux_KefF.
DR   PANTHER; PTHR10204:SF50; PTHR10204:SF50; 1.
DR   Pfam; PF02525; Flavodoxin_2; 1.
DR   SUPFAM; SSF52218; SSF52218; 1.
PE   3: Inferred from homology;
DR   PRODOM; B1XC47.
DR   SWISS-2DPAGE; B1XC47.
KW   Cell inner membrane; Cell membrane; Flavoprotein; FMN; Membrane; NAD;
KW   Oxidoreductase.
FT   CHAIN         1    176       Glutathione-regulated potassium-efflux
FT                                system ancillary protein KefF.
FT                                /FTId=PRO_1000145557.
FT   NP_BIND      14     17       FMN. {ECO:0000255|HAMAP-Rule:MF_01414}.
FT   NP_BIND      65     68       FMN. {ECO:0000255|HAMAP-Rule:MF_01414}.
FT   NP_BIND     105    108       FMN. {ECO:0000255|HAMAP-Rule:MF_01414}.
FT   BINDING       8      8       FMN. {ECO:0000255|HAMAP-Rule:MF_01414}.
SQ   SEQUENCE   176 AA;  20170 MW;  F641E3952F4EFC41 CRC64;
     MILIIYAHPY PHHSHANKRM LEQARTLEGV EIRSLYQLYP DFNIDIAAEQ EALSRADLIV
     WQHPMQWYSI PPLLKLWIDK VFSHGWAYGH GGTALHGKHL LWAVTTGGGE SHFEIGAHPG
     FDVLSQPLQA TAIYCGLNWL PPFAMHCTFI CDDETLEGQA RHYKQRLLEW QEAHHG
//

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