(data stored in ACNUC7421 zone)

SWISSPROT: SFGH1_ECODH

ID   SFGH1_ECODH             Reviewed;         277 AA.
AC   B1XEU8;
DT   01-JUL-2008, integrated into UniProtKB/Swiss-Prot.
DT   20-MAY-2008, sequence version 1.
DT   05-JUL-2017, entry version 51.
DE   RecName: Full=S-formylglutathione hydrolase FrmB;
DE            Short=FGH;
DE            EC=3.1.2.12;
GN   Name=frmB; OrderedLocusNames=ECDH10B_0310;
OS   Escherichia coli (strain K12 / DH10B).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=316385;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / DH10B;
RX   PubMed=18245285; DOI=10.1128/JB.01695-07;
RA   Durfee T., Nelson R., Baldwin S., Plunkett G. III, Burland V., Mau B.,
RA   Petrosino J.F., Qin X., Muzny D.M., Ayele M., Gibbs R.A., Csorgo B.,
RA   Posfai G., Weinstock G.M., Blattner F.R.;
RT   "The complete genome sequence of Escherichia coli DH10B: insights into
RT   the biology of a laboratory workhorse.";
RL   J. Bacteriol. 190:2597-2606(2008).
CC   -!- FUNCTION: Serine hydrolase involved in the detoxification of
CC       formaldehyde. Hydrolyzes S-formylglutathione to glutathione and
CC       formate (By similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY: S-formylglutathione + H(2)O = glutathione +
CC       formate.
CC   -!- SIMILARITY: Belongs to the esterase D family. {ECO:0000305}.
DR   EMBL; CP000948; ACB01488.1; -; Genomic_DNA.
DR   RefSeq; WP_000419081.1; NC_010473.1.
DR   ProteinModelPortal; B1XEU8; -.
DR   SMR; B1XEU8; -.
DR   ESTHER; ecoli-yaim; A85-EsteraseD-FGH.
DR   EnsemblBacteria; ACB01488; ACB01488; ECDH10B_0310.
DR   KEGG; ecd:ECDH10B_0310; -.
DR   eggNOG; ENOG4105C4W; Bacteria.
DR   eggNOG; COG0627; LUCA.
DR   HOGENOM; HOG000263929; -.
DR   KO; K01070; -.
DR   OMA; HACFGGW; -.
DR   GO; GO:0052689; F:carboxylic ester hydrolase activity; IEA:UniProtKB-KW.
DR   GO; GO:0018738; F:S-formylglutathione hydrolase activity; IEA:UniProtKB-EC.
DR   GO; GO:0046294; P:formaldehyde catabolic process; IEA:InterPro.
DR   Gene3D; 3.40.50.1820; -; 1.
DR   InterPro; IPR029058; AB_hydrolase.
DR   InterPro; IPR000801; Esterase_put.
DR   InterPro; IPR014186; S-formylglutathione_hydrol.
DR   PANTHER; PTHR10061; PTHR10061; 1.
DR   Pfam; PF00756; Esterase; 1.
DR   SUPFAM; SSF53474; SSF53474; 1.
DR   TIGRFAMs; TIGR02821; fghA_ester_D; 1.
PE   3: Inferred from homology;
DR   PRODOM; B1XEU8.
DR   SWISS-2DPAGE; B1XEU8.
KW   Hydrolase; Serine esterase.
FT   CHAIN         1    277       S-formylglutathione hydrolase FrmB.
FT                                /FTId=PRO_0000341655.
FT   ACT_SITE    145    145       Charge relay system. {ECO:0000250}.
FT   ACT_SITE    221    221       Charge relay system. {ECO:0000250}.
FT   ACT_SITE    254    254       Charge relay system. {ECO:0000250}.
SQ   SEQUENCE   277 AA;  31424 MW;  C69C708293FA0AEC CRC64;
     MELIEKHVSF GGWQNMYRHY SQSLKCEMNV GVYLPPKAAN EKLPVLYWLS GLTCNEQNFI
     TKSGMQRYAA EHNIIVVAPD TSPRGSHVAD ADRYDLGQGA GFYLNATQAP WNEHYKMYDY
     IRNELPDLVM HHFPATAKKS ISGHSMGGLG ALVLALRNPD EYVSVSAFSP IVSPSQVPWG
     QQAFAAYLAE NKDAWLDYDP VSLISQGQRV AEIMVDQGLS DDFYAEQLRT PNLEKICQEM
     NIKTLIRYHE GYDHSYYFVS SFIGEHIAYH ANKLNMR
//

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