(data stored in ACNUC7421 zone)

SWISSPROT: RLMH_ECODH

ID   RLMH_ECODH              Reviewed;         155 AA.
AC   B1X631;
DT   24-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT   20-MAY-2008, sequence version 1.
DT   05-JUL-2017, entry version 60.
DE   RecName: Full=Ribosomal RNA large subunit methyltransferase H {ECO:0000255|HAMAP-Rule:MF_00658};
DE            EC=2.1.1.177 {ECO:0000255|HAMAP-Rule:MF_00658};
DE   AltName: Full=23S rRNA (pseudouridine1915-N3)-methyltransferase {ECO:0000255|HAMAP-Rule:MF_00658};
DE   AltName: Full=23S rRNA m3Psi1915 methyltransferase {ECO:0000255|HAMAP-Rule:MF_00658};
DE   AltName: Full=rRNA (pseudouridine-N3-)-methyltransferase RlmH {ECO:0000255|HAMAP-Rule:MF_00658};
GN   Name=rlmH1 {ECO:0000255|HAMAP-Rule:MF_00658};
GN   OrderedLocusNames=ECDH10B_0597;
GN   and
GN   Name=rlmH2 {ECO:0000255|HAMAP-Rule:MF_00658};
GN   OrderedLocusNames=ECDH10B_0705;
OS   Escherichia coli (strain K12 / DH10B).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=316385;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / DH10B;
RX   PubMed=18245285; DOI=10.1128/JB.01695-07;
RA   Durfee T., Nelson R., Baldwin S., Plunkett G. III, Burland V., Mau B.,
RA   Petrosino J.F., Qin X., Muzny D.M., Ayele M., Gibbs R.A., Csorgo B.,
RA   Posfai G., Weinstock G.M., Blattner F.R.;
RT   "The complete genome sequence of Escherichia coli DH10B: insights into
RT   the biology of a laboratory workhorse.";
RL   J. Bacteriol. 190:2597-2606(2008).
CC   -!- FUNCTION: Specifically methylates the pseudouridine at position
CC       1915 (m3Psi1915) in 23S rRNA. {ECO:0000255|HAMAP-Rule:MF_00658}.
CC   -!- CATALYTIC ACTIVITY: S-adenosyl-L-methionine + pseudouridine(1915)
CC       in 23S rRNA = S-adenosyl-L-homocysteine + N(3)-
CC       methylpseudouridine(1915) in 23S rRNA. {ECO:0000255|HAMAP-
CC       Rule:MF_00658}.
CC   -!- SUBUNIT: Homodimer. {ECO:0000255|HAMAP-Rule:MF_00658}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00658}.
CC   -!- SIMILARITY: Belongs to the RNA methyltransferase RlmH family.
CC       {ECO:0000255|HAMAP-Rule:MF_00658}.
DR   EMBL; CP000948; ACB01757.1; -; Genomic_DNA.
DR   EMBL; CP000948; ACB01858.1; -; Genomic_DNA.
DR   RefSeq; WP_000776104.1; NC_010473.1.
DR   ProteinModelPortal; B1X631; -.
DR   SMR; B1X631; -.
DR   EnsemblBacteria; ACB01757; ACB01757; ECDH10B_0597.
DR   EnsemblBacteria; ACB01858; ACB01858; ECDH10B_0705.
DR   KEGG; ecd:ECDH10B_0597; -.
DR   KEGG; ecd:ECDH10B_0705; -.
DR   eggNOG; ENOG4108UXA; Bacteria.
DR   eggNOG; COG1576; LUCA.
DR   HOGENOM; HOG000218434; -.
DR   KO; K00783; -.
DR   OMA; NGEPYHK; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0008168; F:methyltransferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0006364; P:rRNA processing; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.1280.10; -; 1.
DR   HAMAP; MF_00658; 23SrRNA_methyltr_H; 1.
DR   InterPro; IPR029028; Alpha/beta_knot_MTases.
DR   InterPro; IPR003742; RlmH-like.
DR   InterPro; IPR029026; tRNA_m1G_MTases_N.
DR   PANTHER; PTHR33603:SF2; PTHR33603:SF2; 1.
DR   Pfam; PF02590; SPOUT_MTase; 1.
DR   PIRSF; PIRSF004505; MT_bac; 1.
DR   SUPFAM; SSF75217; SSF75217; 1.
DR   TIGRFAMs; TIGR00246; tRNA_RlmH_YbeA; 1.
PE   3: Inferred from homology;
DR   PRODOM; B1X631.
DR   SWISS-2DPAGE; B1X631.
KW   Cytoplasm; Methyltransferase; rRNA processing;
KW   S-adenosyl-L-methionine; Transferase.
FT   CHAIN         1    155       Ribosomal RNA large subunit
FT                                methyltransferase H.
FT                                /FTId=PRO_0000366592.
SQ   SEQUENCE   155 AA;  17341 MW;  DFA0EFCF4EB057E7 CRC64;
     MKLQLVAVGT KMPDWVQTGF TEYLRRFPKD MPFELIEIPA GKRGKNADIK RILDKEGEQM
     LAAAGKNRIV TLDIPGKPWD TPQLAAELER WKLDGRDVSL LIGGPEGLSP ACKAAAEQSW
     SLSALTLPHP LVRVLVAESL YRAWSITTNH PYHRE
//

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