(data stored in ACNUC29543 zone)

SWISSPROT: B2JMP0_PARP8

ID   B2JMP0_PARP8            Unreviewed;       366 AA.
AC   B2JMP0;
DT   10-JUN-2008, integrated into UniProtKB/TrEMBL.
DT   10-JUN-2008, sequence version 1.
DT   08-MAY-2019, entry version 68.
DE   RecName: Full=tRNA 2-selenouridine/geranyl-2-thiouridine synthase {ECO:0000256|SAAS:SAAS01064493};
DE            EC=2.5.1.- {ECO:0000256|SAAS:SAAS01064486};
DE            EC=2.9.1.- {ECO:0000256|SAAS:SAAS00356069};
GN   OrderedLocusNames=Bphy_3699 {ECO:0000313|EMBL:ACC72834.1};
OS   Paraburkholderia phymatum (strain DSM 17167 / CIP 108236 / LMG 21445 /
OS   STM815) (Burkholderia phymatum).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Burkholderiaceae; Paraburkholderia.
OX   NCBI_TaxID=391038 {ECO:0000313|EMBL:ACC72834.1, ECO:0000313|Proteomes:UP000001192};
RN   [1] {ECO:0000313|Proteomes:UP000001192}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 17167 / CIP 108236 / LMG 21445 / STM815
RC   {ECO:0000313|Proteomes:UP000001192};
RX   PubMed=25197461; DOI=10.4056/sigs.4861021;
RA   Moulin L., Klonowska A., Caroline B., Booth K., Vriezen J.A.,
RA   Melkonian R., James E.K., Young J.P., Bena G., Hauser L., Land M.,
RA   Kyrpides N., Bruce D., Chain P., Copeland A., Pitluck S., Woyke T.,
RA   Lizotte-Waniewski M., Bristow J., Riley M.;
RT   "Complete genome sequence of Burkholderia phymatum STM815(T), a broad
RT   host range and efficient nitrogen-fixing symbiont of Mimosa species.";
RL   Stand. Genomic Sci. 9:763-774(2014).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(2E)-geranyl diphosphate + 5-methylaminomethyl-2-
CC         thiouridine(34) in tRNA = 5-methylaminomethyl-S-(2E)-geranyl-
CC         thiouridine(34) in tRNA + diphosphate; Xref=Rhea:RHEA:14085,
CC         Rhea:RHEA-COMP:10195, Rhea:RHEA-COMP:14654, ChEBI:CHEBI:33019,
CC         ChEBI:CHEBI:58057, ChEBI:CHEBI:74455, ChEBI:CHEBI:140632;
CC         Evidence={ECO:0000256|SAAS:SAAS01120034};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=5-methylaminomethyl-2-thiouridine(34) in tRNA + H(+) +
CC         selenophosphate = 5-methylaminomethyl-2-selenouridine(34) in
CC         tRNA + thiophosphate; Xref=Rhea:RHEA:42716, Rhea:RHEA-
CC         COMP:10195, Rhea:RHEA-COMP:10196, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:16144, ChEBI:CHEBI:74455, ChEBI:CHEBI:74752,
CC         ChEBI:CHEBI:82743; Evidence={ECO:0000256|SAAS:SAAS01120033};
CC   -!- SUBUNIT: Monomer. {ECO:0000256|SAAS:SAAS00356061}.
CC   -!- SIMILARITY: Belongs to the SelU family.
CC       {ECO:0000256|SAAS:SAAS01064488}.
CC   -----------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   -----------------------------------------------------------------------
DR   EMBL; CP001044; ACC72834.1; -; Genomic_DNA.
DR   STRING; 391038.Bphy_3699; -.
DR   EnsemblBacteria; ACC72834; ACC72834; Bphy_3699.
DR   KEGG; bph:Bphy_3699; -.
DR   eggNOG; ENOG4105CR7; Bacteria.
DR   eggNOG; COG2603; LUCA.
DR   HOGENOM; HOG000260001; -.
DR   KO; K06917; -.
DR   OMA; RPLVYCW; -.
DR   Proteomes; UP000001192; Chromosome 2.
DR   GO; GO:0043828; F:tRNA 2-selenouridine synthase activity; IEA:InterPro.
DR   GO; GO:0070329; P:tRNA seleno-modification; IEA:InterPro.
DR   Gene3D; 3.40.250.10; -; 1.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR001763; Rhodanese-like_dom.
DR   InterPro; IPR036873; Rhodanese-like_dom_sf.
DR   InterPro; IPR017582; tRNA_2-selenouridine_synthase.
DR   Pfam; PF00581; Rhodanese; 1.
DR   SMART; SM00450; RHOD; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   SUPFAM; SSF52821; SSF52821; 1.
DR   TIGRFAMs; TIGR03167; tRNA_sel_U_synt; 1.
DR   PROSITE; PS50206; RHODANESE_3; 1.
PE   3: Inferred from homology;
DR   PRODOM; B2JMP0.
DR   SWISS-2DPAGE; B2JMP0.
KW   Complete proteome {ECO:0000313|Proteomes:UP000001192};
KW   Reference proteome {ECO:0000313|Proteomes:UP000001192};
KW   Selenium {ECO:0000256|SAAS:SAAS00433829};
KW   Transferase {ECO:0000256|SAAS:SAAS00433820}.
FT   DOMAIN       29    151       Rhodanese. {ECO:0000259|PROSITE:PS50206}.
SQ   SEQUENCE   366 AA;  41419 MW;  A1E2F1C521300FAA CRC64;
     MHAIIELTSR RDRSLLLPLK SLLAPLDQIA DFDEIVDVRT PLEFAEDHIP GAINAPVLSN
     EERVIIGTMY KQVSPFEATR QGAAMVSRNI AAHLETTFAD RPRNWRPLVY CWRGGKRSGS
     MTTWFNLIGW RARQLDGGYK TYRRSVVELL ETLPQQFRYI VLAGHTGSGK TRLLNALAGA
     DAQTLDLEAL AAHRGSILGM LPDEAQPSQK AFDTALIGAL RGFDANQPVF VEAESRRIGA
     IALPVSLTEQ IHRGTCIRVE TERDERIRLL LQDYAHLFDN RALFKSQLAR LVELHGHARI
     EEWHKLIDED RRVELFEQLI DLHYDPAYAR STGKHFAKLP ESTHFVLRPT DADLHEQACA
     LLARLR
//

If you have problems or comments...

PBIL Back to PBIL home page