(data stored in ACNUC7421 zone)

SWISSPROT: B2TEE3_PARPJ

ID   B2TEE3_PARPJ            Unreviewed;       742 AA.
AC   B2TEE3;
DT   01-JUL-2008, integrated into UniProtKB/TrEMBL.
DT   01-JUL-2008, sequence version 1.
DT   08-MAY-2019, entry version 76.
DE   RecName: Full=Isocitrate dehydrogenase [NADP] {ECO:0000256|PIRNR:PIRNR009407};
DE            EC=1.1.1.42 {ECO:0000256|PIRNR:PIRNR009407};
DE   AltName: Full=Oxalosuccinate decarboxylase {ECO:0000256|PIRNR:PIRNR009407};
GN   OrderedLocusNames=Bphyt_4080 {ECO:0000313|EMBL:ACD18464.1};
OS   Paraburkholderia phytofirmans (strain DSM 17436 / LMG 22146 / PsJN)
OS   (Burkholderia phytofirmans).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Burkholderiaceae; Paraburkholderia.
OX   NCBI_TaxID=398527 {ECO:0000313|EMBL:ACD18464.1, ECO:0000313|Proteomes:UP000001739};
RN   [1] {ECO:0000313|EMBL:ACD18464.1, ECO:0000313|Proteomes:UP000001739}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 17436 / LMG 22146 / PsJN
RC   {ECO:0000313|Proteomes:UP000001739};
RX   PubMed=21551308; DOI=10.1128/JB.05055-11;
RA   Weilharter A., Mitter B., Shin M.V., Chain P.S., Nowak J.,
RA   Sessitsch A.;
RT   "Complete genome sequence of the plant growth-promoting endophyte
RT   Burkholderia phytofirmans strain PsJN.";
RL   J. Bacteriol. 193:3383-3384(2011).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=isocitrate + NADP(+) = 2-oxoglutarate + CO2 + NADPH;
CC         Xref=Rhea:RHEA:19629, ChEBI:CHEBI:16087, ChEBI:CHEBI:16526,
CC         ChEBI:CHEBI:16810, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349;
CC         EC=1.1.1.42; Evidence={ECO:0000256|PIRNR:PIRNR009407};
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000256|PIRSR:PIRSR009407-3};
CC       Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC         Evidence={ECO:0000256|PIRSR:PIRSR009407-3};
CC       Note=Binds 1 Mg(2+) or Mn(2+) ion per subunit.
CC       {ECO:0000256|PIRSR:PIRSR009407-3};
CC   -!- SIMILARITY: Belongs to the monomeric-type IDH family.
CC       {ECO:0000256|PIRNR:PIRNR009407}.
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DR   EMBL; CP001053; ACD18464.1; -; Genomic_DNA.
DR   RefSeq; WP_012425980.1; NC_010676.1.
DR   STRING; 398527.Bphyt_4080; -.
DR   EnsemblBacteria; ACD18464; ACD18464; Bphyt_4080.
DR   KEGG; bpy:Bphyt_4080; -.
DR   eggNOG; ENOG4105E9K; Bacteria.
DR   eggNOG; COG2838; LUCA.
DR   HOGENOM; HOG000240860; -.
DR   KO; K00031; -.
DR   OMA; RDSGKMW; -.
DR   OrthoDB; 222705at2; -.
DR   BioCyc; BPHY398527:GJEX-4078-MONOMER; -.
DR   Proteomes; UP000001739; Chromosome 2.
DR   GO; GO:0004450; F:isocitrate dehydrogenase (NADP+) activity; IEA:UniProtKB-EC.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0006097; P:glyoxylate cycle; IEA:UniProtKB-KW.
DR   GO; GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-KW.
DR   InterPro; IPR004436; Isocitrate_DH_NADP_mono.
DR   PANTHER; PTHR36999; PTHR36999; 1.
DR   Pfam; PF03971; IDH; 1.
DR   PIRSF; PIRSF009407; IDH_monmr; 1.
DR   TIGRFAMs; TIGR00178; monomer_idh; 1.
PE   3: Inferred from homology;
DR   PRODOM; B2TEE3.
DR   SWISS-2DPAGE; B2TEE3.
KW   Complete proteome {ECO:0000313|Proteomes:UP000001739};
KW   Glyoxylate bypass {ECO:0000256|PIRNR:PIRNR009407};
KW   Magnesium {ECO:0000256|PIRSR:PIRSR009407-3};
KW   Metal-binding {ECO:0000256|PIRSR:PIRSR009407-3};
KW   NADP {ECO:0000256|PIRNR:PIRNR009407, ECO:0000256|PIRSR:PIRSR009407-4};
KW   Oxidoreductase {ECO:0000256|PIRNR:PIRNR009407,
KW   ECO:0000313|EMBL:ACD18464.1};
KW   Tricarboxylic acid cycle {ECO:0000256|PIRNR:PIRNR009407}.
FT   NP_BIND      83     88       NADP. {ECO:0000256|PIRSR:PIRSR009407-4}.
FT   NP_BIND     585    586       NADP. {ECO:0000256|PIRSR:PIRSR009407-4}.
FT   NP_BIND     601    603       NADP. {ECO:0000256|PIRSR:PIRSR009407-4}.
FT   REGION      133    140       Substrate binding. {ECO:0000256|PIRSR:
FT                                PIRSR009407-2}.
FT   METAL       351    351       Magnesium or manganese.
FT                                {ECO:0000256|PIRSR:PIRSR009407-3}.
FT   METAL       549    549       Magnesium or manganese.
FT                                {ECO:0000256|PIRSR:PIRSR009407-3}.
FT   METAL       553    553       Magnesium or manganese.
FT                                {ECO:0000256|PIRSR:PIRSR009407-3}.
FT   BINDING     136    136       NADP. {ECO:0000256|PIRSR:PIRSR009407-4}.
FT   BINDING     146    146       Substrate. {ECO:0000256|PIRSR:
FT                                PIRSR009407-2}.
FT   BINDING     548    548       Substrate. {ECO:0000256|PIRSR:
FT                                PIRSR009407-2}.
FT   BINDING     590    590       NADP. {ECO:0000256|PIRSR:PIRSR009407-4}.
FT   BINDING     650    650       NADP. {ECO:0000256|PIRSR:PIRSR009407-4}.
FT   SITE        256    256       Critical for catalysis.
FT                                {ECO:0000256|PIRSR:PIRSR009407-1}.
FT   SITE        421    421       Critical for catalysis.
FT                                {ECO:0000256|PIRSR:PIRSR009407-1}.
SQ   SEQUENCE   742 AA;  79670 MW;  EF1254919EF51221 CRC64;
     MSTPPKIIYT LTDEAPALAT YSLLPIVKAF TRSSDVIVET RDISLAGRII AAFPDYLSAE
     QKGPDDLAEL GGLSTRPEAN VIKLPNISAS VPQLKAAITE LRDQGYKLPA YPDVANTDAE
     KDVKARYDKI KGSAVNPVLR EGNSDRRAPL SVKNYARKHP HKMGAWSADS KSHVAHMSGG
     DFYGSEKSAL IGAAGSVKIE LTATDGSKTV LKEKTAVQAG EIIDASVLSK NALRSFIEAE
     IADAKAKGVL FSVHLKATMM KVSDPIIFGH VVSVFYKDVL TKHADALAQA GFNPNNGIGD
     LYARLKDLPA ETVAAIEADI KAQYEQRPQL AMVNSDKGIT SLHVPSDVIV DASMPAMIRE
     SGKMWGADGA LHDAKAVIPD RCYAGVYQAV IEDCKKHGAF DPVTMGTVPN VGLMAQAAEE
     YGSHDKTFQI PANGVVRVTD AAGTVLIEQQ VEAGDIWRMC QTKDAPVQDW VKLAVNRARA
     TNTPAVFWLD AARAHDAQII KKVEQYLKNH DTTGLDIRVM TPVEATKFSI ERIRAGKDTI
     SVTGNVLRDY LTDLFPIMEL GTSAKMLSIV PLMAGGGMFE TGAGGSAPKH VQQLVEEGFL
     RWDSLGEFLA LAASLEHLSS AYHNPKAQVL AKTLDQATGK FLDNDKSPAR KVGGIDNRGS
     HFYLALYWAE ALAAQTEDAA LQAQFAGVAK ALAENEAKIV EELAAAQGKP VDIGGYYRPN
     VELTSKAMRP SATLNKIVDA IA
//

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